SCRK2_ORYSJ
ID SCRK2_ORYSJ Reviewed; 336 AA.
AC Q0J8G4; B7EHD2; Q7XXS9; Q944F5;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Fructokinase-2;
DE EC=2.7.1.4;
DE AltName: Full=Fructokinase II;
DE AltName: Full=OsFKII;
GN Name=FRK2; Synonyms=FKII; OrderedLocusNames=Os08g0113100, LOC_Os08g02120;
GN ORFNames=OsJ_024778, P0498H04.29;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Panicle;
RX PubMed=15659629; DOI=10.1105/tpc.104.028456;
RA Moriguchi K., Suzuki T., Ito Y., Yamazaki Y., Niwa Y., Kurata N.;
RT "Functional isolation of novel nuclear proteins showing a variety of
RT subnuclear localizations.";
RL Plant Cell 17:389-403(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 3-9, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=cv. Nipponbare;
RX PubMed=16758443; DOI=10.1002/pmic.200600043;
RA Nozu Y., Tsugita A., Kamijo K.;
RT "Proteomic analysis of rice leaf, stem and root tissues during growth
RT course.";
RL Proteomics 6:3665-3670(2006).
RN [8]
RP PROTEIN SEQUENCE OF 4-13; 118-127 AND 214-223, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare; TISSUE=Anther, Panicle, Sheath, and Stem;
RX PubMed=14681440; DOI=10.1093/nar/gkh020;
RA Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.;
RT "Rice proteome database based on two-dimensional polyacrylamide gel
RT electrophoresis: its status in 2003.";
RL Nucleic Acids Res. 32:D388-D392(2004).
RN [9]
RP BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=12475618; DOI=10.1016/s0031-9422(02)00428-4;
RA Jiang H., Dian W., Liu F., Wu P.;
RT "Isolation and characterization of two fructokinase cDNA clones from
RT rice.";
RL Phytochemistry 62:47-52(2003).
CC -!- FUNCTION: May play an important role in maintaining the flux of carbon
CC towards starch formation in endosperm. May also be involved in a sugar-
CC sensing pathway.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.4;
CC -!- ACTIVITY REGULATION: Strongly inhibited at high fructose concentration.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.34 mM for fructose {ECO:0000269|PubMed:12475618};
CC -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC -!- TISSUE SPECIFICITY: Expressed in stem, sheaths, anthers, and panicles
CC (at protein level). {ECO:0000269|PubMed:14681440}.
CC -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC {ECO:0000305}.
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DR EMBL; AB110164; BAC78556.1; -; mRNA.
DR EMBL; AP004566; BAD09515.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF22751.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT03524.1; -; Genomic_DNA.
DR EMBL; CM000145; EAZ41295.1; -; Genomic_DNA.
DR EMBL; AK070120; BAG91779.1; -; mRNA.
DR EMBL; AK099353; BAG94082.1; -; mRNA.
DR RefSeq; XP_015650262.1; XM_015794776.1.
DR AlphaFoldDB; Q0J8G4; -.
DR SMR; Q0J8G4; -.
DR STRING; 4530.OS08T0113100-01; -.
DR PaxDb; Q0J8G4; -.
DR PRIDE; Q0J8G4; -.
DR EnsemblPlants; Os08t0113100-01; Os08t0113100-01; Os08g0113100.
DR GeneID; 4344497; -.
DR Gramene; Os08t0113100-01; Os08t0113100-01; Os08g0113100.
DR KEGG; osa:4344497; -.
DR eggNOG; KOG2855; Eukaryota.
DR HOGENOM; CLU_027634_6_1_1; -.
DR InParanoid; Q0J8G4; -.
DR OMA; AIVKQGP; -.
DR OrthoDB; 918144at2759; -.
DR BRENDA; 2.7.1.4; 4460.
DR UniPathway; UPA00152; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR ExpressionAtlas; Q0J8G4; baseline and differential.
DR Genevisible; Q0J8G4; OS.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IBA:GO_Central.
DR GO; GO:0006000; P:fructose metabolic process; IBA:GO_Central.
DR GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.1190.20; -; 1.
DR InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR InterPro; IPR011611; PfkB_dom.
DR InterPro; IPR029056; Ribokinase-like.
DR Pfam; PF00294; PfkB; 1.
DR SUPFAM; SSF53613; SSF53613; 1.
DR PROSITE; PS00583; PFKB_KINASES_1; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Carbohydrate metabolism; Direct protein sequencing; Kinase;
KW Nucleotide-binding; Reference proteome; Transferase.
FT CHAIN 1..336
FT /note="Fructokinase-2"
FT /id="PRO_0000234065"
SQ SEQUENCE 336 AA; 35517 MW; FFC33297A2AC1A92 CRC64;
MAPLGDGAAA AAAAEPNLVV SFGEMLIDFV PDVAGVSLAE SGGFVKAPGG APANVACAIS
KLGGSSAFVG KFGDDEFGHM LVDILKKNGV NAEGCLFDEH ARTALAFVTL KSNGEREFMF
YRNPSADMLL TEAELNLDLI RRAKIFHYGS ISLITEPCRS AHVAAMRAAK SAGILCSYDP
NVRLPLWPSE DAARAGILSI WKEADFIKVS DDEVAFLTQG DANDEKNVLS LWFDGLKLLI
VTDGEKGCRY FTKDFKGSVP GFSVNTVDTT GAGDAFVGSL LVNVAKDDSI FHNEEKLREA
LKFSNACGAI CTTKKGAIPA LPTVAVAQEL ISKAAN
