SCRK6_ARATH
ID SCRK6_ARATH Reviewed; 384 AA.
AC Q9C524; Q8H119; Q94K38;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Probable fructokinase-6, chloroplastic;
DE EC=2.7.1.4;
DE Flags: Precursor;
GN OrderedLocusNames=At1g66430 {ECO:0000312|Araport:AT1G66430};
GN ORFNames=F28G11.11 {ECO:0000312|EMBL:AAG51160.1},
GN T27F4.17 {ECO:0000312|EMBL:AAG52172.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 142-384.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: May play an important role in maintaining the flux of carbon
CC towards starch formation. {ECO:0000250|UniProtKB:Q6XZ79}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.4;
CC -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC {ECO:0000255|RuleBase:RU003704}.
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DR EMBL; AC020665; AAG52172.1; -; Genomic_DNA.
DR EMBL; AC074025; AAG51160.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34508.1; -; Genomic_DNA.
DR EMBL; BX816698; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AF370329; AAK44144.2; -; mRNA.
DR EMBL; BT000889; AAN41289.1; -; mRNA.
DR PIR; G96689; G96689.
DR RefSeq; NP_564875.2; NM_105314.5.
DR AlphaFoldDB; Q9C524; -.
DR SMR; Q9C524; -.
DR BioGRID; 28182; 1.
DR IntAct; Q9C524; 2.
DR STRING; 3702.AT1G66430.1; -.
DR PaxDb; Q9C524; -.
DR PRIDE; Q9C524; -.
DR ProteomicsDB; 232963; -.
DR EnsemblPlants; AT1G66430.1; AT1G66430.1; AT1G66430.
DR GeneID; 842961; -.
DR Gramene; AT1G66430.1; AT1G66430.1; AT1G66430.
DR KEGG; ath:AT1G66430; -.
DR Araport; AT1G66430; -.
DR TAIR; locus:2028987; AT1G66430.
DR eggNOG; KOG2855; Eukaryota.
DR HOGENOM; CLU_027634_6_1_1; -.
DR InParanoid; Q9C524; -.
DR OMA; NWRPTFW; -.
DR OrthoDB; 918144at2759; -.
DR PhylomeDB; Q9C524; -.
DR BioCyc; ARA:AT1G66430-MON; -.
DR BRENDA; 2.7.1.4; 399.
DR UniPathway; UPA00152; -.
DR PRO; PR:Q9C524; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C524; baseline and differential.
DR Genevisible; Q9C524; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IDA:TAIR.
DR GO; GO:0016051; P:carbohydrate biosynthetic process; IGI:TAIR.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IGI:TAIR.
DR GO; GO:0006000; P:fructose metabolic process; IDA:TAIR.
DR GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.1190.20; -; 1.
DR InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR InterPro; IPR011611; PfkB_dom.
DR InterPro; IPR002139; Ribo/fructo_kinase.
DR InterPro; IPR029056; Ribokinase-like.
DR Pfam; PF00294; PfkB; 1.
DR PRINTS; PR00990; RIBOKINASE.
DR SUPFAM; SSF53613; SSF53613; 1.
DR PROSITE; PS00583; PFKB_KINASES_1; 1.
DR PROSITE; PS00584; PFKB_KINASES_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Carbohydrate metabolism; Chloroplast; Kinase;
KW Nucleotide-binding; Plastid; Reference proteome; Transferase;
KW Transit peptide.
FT TRANSIT 1..46
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 47..384
FT /note="Probable fructokinase-6, chloroplastic"
FT /id="PRO_0000430866"
FT REGION 34..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 304
FT /note="S -> R (in Ref. 3; BX816698)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 384 AA; 41471 MW; 8873EC5152F65928 CRC64;
MALQATTTTF CFSGPTFRST PHSLTSKRPI SIKATTSSPS RLSNSRSNLK GRALSSDGST
QESPYVVCFG EMLIDFVPTT SGLSLADAPA FKKAPGGAPA NVAVGIARLG GSSAFIGKVG
EDEFGYMLAN ILKDNNVNND GMRFDPGART ALAFVTLTNE GEREFMFYRN PSADMLLEES
ELDFDLIKKA KIFHYGSISL ITEPCKSAHI SAAKAAKEAG VILSYDPNLR LPLWPSADNA
REEILSIWET ADIIKISEEE IVFLTKGEDP YDDNVVRKLF HPKLKLLLVT EGPEGCRYYT
KDFSGRVHGL KVDVVDTTGA GDAFVAGILS QLANDLSLLQ DEERLREALM FANACGALTV
KVRGAIPALP TKEAVHEALL KAVV