ID   SCRK_FUSMR              Reviewed;          27 AA.
AC   Q09123;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Fructokinase;
DE            EC=2.7.1.4;
DE   Flags: Fragment;
GN   Name=scrK {ECO:0000250|UniProtKB:O05510};
OS   Fusobacterium mortiferum.
OC   Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium.
OX   NCBI_TaxID=850;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL
RP   PROPERTIES, SUBUNIT, AND INDUCTION.
RC   STRAIN=ATCC 25557 / DSM 19809 / CCUG 14475 / VPI 4123A;
RX   PubMed=1533618; DOI=10.1128/jb.174.10.3227-3235.1992;
RA   Thompson J., Nguyen N.Y., Robrish S.A.;
RT   "Sucrose fermentation by Fusobacterium mortiferum ATCC 25557: transport,
RT   catabolism, and products.";
RL   J. Bacteriol. 174:3227-3235(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.4;
CC         Evidence={ECO:0000269|PubMed:1533618};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:1533618};
CC       Note=Divalent metal cations. Maximum activity was observed with Mg(2+).
CC       {ECO:0000269|PubMed:1533618};
CC   -!- ACTIVITY REGULATION: Inhibition by zinc ions.
CC       {ECO:0000250|UniProtKB:O05510, ECO:0000305}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.1 mM for ATP {ECO:0000269|PubMed:1533618};
CC         KM=1.8 mM for magnesium {ECO:0000269|PubMed:1533618};
CC         KM=0.4 mM for fructose {ECO:0000269|PubMed:1533618};
CC         Vmax=74 umol/min/mg enzyme for fructose 6-phosphate
CC         {ECO:0000269|PubMed:1533618};
CC       pH dependence:
CC         Optimum pH is 6.5-7.5. {ECO:0000269|PubMed:1533618};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:1533618}.
CC   -!- INDUCTION: Induced by sucrose. {ECO:0000269|PubMed:1533618}.
CC   -!- SIMILARITY: Belongs to the ROK (NagC/XylR) family. {ECO:0000305}.
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DR   AlphaFoldDB; Q09123; -.
DR   SMR; Q09123; -.
DR   SABIO-RK; Q09123; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IDA:UniProtKB.
DR   GO; GO:0044275; P:cellular carbohydrate catabolic process; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   ATP-binding; Carbohydrate metabolism; Direct protein sequencing; Kinase;
KW   Magnesium; Metal-binding; Nucleotide-binding; Transferase; Zinc.
FT   CHAIN           1..>27
FT                   /note="Fructokinase"
FT                   /id="PRO_0000095682"
FT   NON_TER         27
FT                   /evidence="ECO:0000303|PubMed:1533618"
SQ   SEQUENCE   27 AA;  2824 MW;  4721ABEF2E7BDCE9 CRC64;
     MIIGAVEAGG TKFVDGVGNE KGEIFER