ID   SCRK_LACLL              Reviewed;          25 AA.
AC   Q09124;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Fructokinase-1;
DE            EC=2.7.1.4;
DE   AltName: Full=Fructokinase I;
DE   Flags: Fragment;
OS   Lactococcus lactis subsp. lactis (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1360;
RN   [1]
RP   PROTEIN SEQUENCE, AND CHARACTERIZATION.
RC   STRAIN=K1;
RX   PubMed=1658003; DOI=10.1016/s0021-9258(18)54617-2;
RA   Thompson J., Sackett D.L., Donkersloot J.A.;
RT   "Purification and properties of fructokinase I from Lactococcus lactis.
RT   Localization of scrK on the sucrose-nisin transposon Tn5306.";
RL   J. Biol. Chem. 266:22626-22633(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.4;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- ACTIVITY REGULATION: Inhibition by zinc ions (Potential). Inactivated
CC       by EDTA. {ECO:0000305}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7.0-8.0.;
CC   -!- SUBUNIT: Homodimer.
CC   -!- MISCELLANEOUS: Two isoforms, fructokinase I and II, have been found in
CC       this organism.
CC   -!- SIMILARITY: Belongs to the ROK (NagC/XylR) family. {ECO:0000305}.
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DR   PIR; A39319; A39319.
DR   AlphaFoldDB; Q09124; -.
DR   BioCyc; MetaCyc:MON-13045; -.
DR   SABIO-RK; Q09124; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   ATP-binding; Carbohydrate metabolism; Direct protein sequencing; Kinase;
KW   Magnesium; Metal-binding; Nucleotide-binding; Transferase; Zinc.
FT   CHAIN           1..>25
FT                   /note="Fructokinase-1"
FT                   /id="PRO_0000095684"
FT   NON_TER         25
SQ   SEQUENCE   25 AA;  2702 MW;  9C2B771CE8D5A1E8 CRC64;
     SVYYGSIEAG GTKFVLAIAD EHFNI