SCRT1_HUMAN
ID SCRT1_HUMAN Reviewed; 348 AA.
AC Q9BWW7; A8MX66; Q96C52;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-FEB-2022, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Transcriptional repressor scratch 1;
DE AltName: Full=Scratch homolog 1 zinc finger protein;
DE Short=SCRT;
DE Short=Scratch 1;
DE Short=hScrt;
GN Name=SCRT1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INTERACTION WITH E-BOX MOTIF, AND VARIANT SER-133.
RC TISSUE=Brain;
RX PubMed=11274425; DOI=10.1073/pnas.051014098;
RA Nakakura E.K., Watkins D.N., Schuebel K.E., Sriuranpong V., Borges M.W.,
RA Nelkin B.D., Ball D.W.;
RT "Mammalian scratch: a neural-specific snail family transcriptional
RT repressor.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4010-4015(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 185-348.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcriptional repressor that binds E-box motif CAGGTG. Can
CC modulate the action of basic helix-loop-helix (bHLH) transcription
CC factors, critical for neuronal differentiation.
CC {ECO:0000269|PubMed:11274425}.
CC -!- SUBUNIT: Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP
CC (via LIM domains) and AJUBA (via LIM domains). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11274425}.
CC -!- TISSUE SPECIFICITY: Brain specific. {ECO:0000269|PubMed:11274425}.
CC -!- DOMAIN: The N-terminal non zinc-finger region mediates the repressor
CC activity.
CC -!- SIMILARITY: Belongs to the snail C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; AY014996; AAK01467.1; -; mRNA.
DR EMBL; CH471162; EAW82125.1; -; Genomic_DNA.
DR EMBL; BC014675; AAH14675.1; -; mRNA.
DR CCDS; CCDS6421.1; -.
DR RefSeq; NP_112599.2; NM_031309.5.
DR AlphaFoldDB; Q9BWW7; -.
DR SMR; Q9BWW7; -.
DR BioGRID; 123667; 25.
DR STRING; 9606.ENSP00000455711; -.
DR iPTMnet; Q9BWW7; -.
DR PhosphoSitePlus; Q9BWW7; -.
DR BioMuta; SCRT1; -.
DR DMDM; 46397014; -.
DR jPOST; Q9BWW7; -.
DR MassIVE; Q9BWW7; -.
DR PaxDb; Q9BWW7; -.
DR PeptideAtlas; Q9BWW7; -.
DR PRIDE; Q9BWW7; -.
DR ProteomicsDB; 79334; -.
DR Antibodypedia; 59727; 29 antibodies from 13 providers.
DR DNASU; 83482; -.
DR Ensembl; ENST00000569446.3; ENSP00000455711.1; ENSG00000261678.3.
DR Ensembl; ENST00000645782.2; ENSP00000496266.1; ENSG00000284923.2.
DR GeneID; 83482; -.
DR KEGG; hsa:83482; -.
DR MANE-Select; ENST00000569446.3; ENSP00000455711.1; NM_031309.6; NP_112599.2.
DR UCSC; uc033ccm.1; human.
DR CTD; 83482; -.
DR DisGeNET; 83482; -.
DR GeneCards; SCRT1; -.
DR HGNC; HGNC:15950; SCRT1.
DR HPA; ENSG00000261678; Tissue enriched (brain).
DR MIM; 605858; gene.
DR neXtProt; NX_Q9BWW7; -.
DR OpenTargets; ENSG00000261678; -.
DR PharmGKB; PA35015; -.
DR VEuPathDB; HostDB:ENSG00000261678; -.
DR eggNOG; KOG2462; Eukaryota.
DR GeneTree; ENSGT00940000154491; -.
DR HOGENOM; CLU_002678_42_3_1; -.
DR InParanoid; Q9BWW7; -.
DR OrthoDB; 1332418at2759; -.
DR PhylomeDB; Q9BWW7; -.
DR TreeFam; TF315515; -.
DR PathwayCommons; Q9BWW7; -.
DR BioGRID-ORCS; 83482; 44 hits in 1088 CRISPR screens.
DR GenomeRNAi; 83482; -.
DR Pharos; Q9BWW7; Tbio.
DR PRO; PR:Q9BWW7; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q9BWW7; protein.
DR Bgee; ENSG00000261678; Expressed in primary visual cortex and 34 other tissues.
DR Genevisible; Q9BWW7; HS.
DR GO; GO:0016604; C:nuclear body; IDA:HPA.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:2001222; P:regulation of neuron migration; IEA:Ensembl.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR029794; SCRATCH1/2.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR24388:SF60; PTHR24388:SF60; 1.
DR Pfam; PF00096; zf-C2H2; 4.
DR SMART; SM00355; ZnF_C2H2; 5.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..348
FT /note="Transcriptional repressor scratch 1"
FT /id="PRO_0000047036"
FT ZN_FING 191..213
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 222..244
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 248..270
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 276..298
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 304..327
FT /note="C2H2-type 5; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..20
FT /note="SNAG domain"
FT /evidence="ECO:0000250"
FT REGION 130..189
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 133
FT /note="A -> S (in dbSNP:rs7013127)"
FT /evidence="ECO:0000269|PubMed:11274425"
FT /id="VAR_045990"
SQ SEQUENCE 348 AA; 35554 MW; D3BB51709C7795DA CRC64;
MPRSFLVKKV KLDAFSSADL ESAYGRARSD LGAPLHDKGY LSDYVGPSSV YDGDAEAALL
KGPSPEPMYA AAVRGELGPA AAGSAPPPTP RPELATAAGG YINGDAAVSE GYAADAFFIT
DGRSRRKASN AGAAAAPSTA SAAAPDGDAG GGGGAGGRSL GSGPGGRGGT RAGAGTEARA
GPGAAGAGGR HACGECGKTY ATSSNLSRHK QTHRSLDSQL ARRCPTCGKV YVSMPAMAMH
LLTHDLRHKC GVCGKAFSRP WLLQGHMRSH TGEKPFGCAH CGKAFADRSN LRAHMQTHSA
FKHFQCKRCK KSFALKSYLN KHYESACFKG GAGGPAAPAP PQLSPVQA
