SCR_DROME
ID SCR_DROME Reviewed; 417 AA.
AC P09077; Q23990; Q24508; Q9V3Q3;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 5.
DT 03-AUG-2022, entry version 199.
DE RecName: Full=Homeotic protein Sex combs reduced;
GN Name=Scr; ORFNames=CG1030;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2565809; DOI=10.1002/j.1460-2075.1989.tb03367.x;
RA Lemotte P.K., Kuroiwa A., Fessler L.I., Gehring W.J.;
RT "The homeotic gene Sex Combs Reduced of Drosophila: gene structure and
RT embryonic expression.";
RL EMBO J. 8:219-227(1989).
RN [2]
RP SEQUENCE REVISION.
RA Baumgartner P.;
RL Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7835693; DOI=10.1016/0378-1119(94)00642-6;
RA Andrew D.J.;
RT "The sex combs reduced gene of Drosophila melanogaster has multiple
RT transcripts.";
RL Gene 152:149-155(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Berkeley;
RA Celniker S.E., Pfeiffer B.D., Knafels J., Martin C.H., Mayeda C.A.,
RA Palazzolo M.J.;
RT "Complete sequence of the Antennapedia complex of Drosophila.";
RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Kaufman T.C.;
RT "Sequence of cDNA of homeodomain protein from sex combs reduced (Scr)
RT gene.";
RL Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 317-390.
RX PubMed=16453653; DOI=10.1002/j.1460-2075.1985.tb04145.x;
RA Kuroiwa A., Kloter U., Baumgartner P., Gehring W.J.;
RT "Cloning of the homeotic Sex Combs Reduced gene in Drosophila and in situ
RT localization of its transcripts.";
RL EMBO J. 4:3757-3764(1985).
RN [9]
RP FUNCTION.
RX PubMed=1743486; DOI=10.1093/genetics/129.2.423;
RA Pattatucci A.M., Otteson D.C., Kaufman T.C.;
RT "A functional and structural analysis of the Sex combs reduced locus of
RT Drosophila melanogaster.";
RL Genetics 129:423-441(1991).
RN [10]
RP REVIEW ON FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12492146; DOI=10.1046/j.1525-142x.2002.02034.x;
RA Hughes C.L., Kaufman T.C.;
RT "Hox genes and the evolution of the arthropod body plan.";
RL Evol. Dev. 4:459-499(2002).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Sequence-specific transcription factor which is part of a
CC developmental regulatory system that provides cells with specific
CC positional identities on the anterior-posterior axis. Controls the
CC segmental transformation of the first to the second thoracic segment
CC (prothorax to mesothorax) and of the labial palps into maxillary palps.
CC In embryo, required for fusion of labial lobes and development of the
CC T1 denticle belt. In adult, expression in the head is necessary for
CC proper development of the labium. In the first thoracic segment of the
CC adult, required for proper development of the sex comb and to suppress
CC improper prothoracic wing development. {ECO:0000269|PubMed:1743486}.
CC -!- INTERACTION:
CC P09077; P40427: exd; NbExp=3; IntAct=EBI-666569, EBI-101537;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: In embryo, expressed in the labial and anterior
CC prothoracic segments and the extreme posterior of the maxillary
CC segment. In adult, expressed in the maxillary analgen of the eye and
CC antennal imaginal disk and the labial and T1 leg imaginal disks.
CC {ECO:0000269|PubMed:12492146}.
CC -!- SIMILARITY: Belongs to the Antp homeobox family. Deformed subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA19240.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA32637.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X14475; CAA32637.1; ALT_FRAME; Genomic_DNA.
DR EMBL; X77075; CAA54348.1; -; Genomic_DNA.
DR EMBL; X77076; CAA54348.1; JOINED; Genomic_DNA.
DR EMBL; AE001572; AAD19795.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54082.1; -; Genomic_DNA.
DR EMBL; U10506; AAA19240.1; ALT_FRAME; mRNA.
DR EMBL; X05228; CAA28857.1; -; Genomic_DNA.
DR PIR; S03631; S03631.
DR RefSeq; NP_524248.2; NM_079524.4.
DR RefSeq; NP_996165.1; NM_206443.2.
DR PDB; 2R5Y; X-ray; 2.60 A; A=298-384.
DR PDB; 2R5Z; X-ray; 2.60 A; A=298-384.
DR PDBsum; 2R5Y; -.
DR PDBsum; 2R5Z; -.
DR AlphaFoldDB; P09077; -.
DR SMR; P09077; -.
DR BioGRID; 66031; 43.
DR DIP; DIP-40017N; -.
DR IntAct; P09077; 1.
DR STRING; 7227.FBpp0089393; -.
DR iPTMnet; P09077; -.
DR PaxDb; P09077; -.
DR PRIDE; P09077; -.
DR DNASU; 40833; -.
DR EnsemblMetazoa; FBtr0081657; FBpp0081163; FBgn0003339.
DR EnsemblMetazoa; FBtr0081658; FBpp0089393; FBgn0003339.
DR GeneID; 40833; -.
DR KEGG; dme:Dmel_CG1030; -.
DR CTD; 109559; -.
DR FlyBase; FBgn0003339; Scr.
DR VEuPathDB; VectorBase:FBgn0003339; -.
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000171134; -.
DR HOGENOM; CLU_034137_0_0_1; -.
DR InParanoid; P09077; -.
DR OMA; GANDYFP; -.
DR OrthoDB; 1556098at2759; -.
DR PhylomeDB; P09077; -.
DR SignaLink; P09077; -.
DR BioGRID-ORCS; 40833; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Scr; fly.
DR EvolutionaryTrace; P09077; -.
DR GenomeRNAi; 40833; -.
DR PRO; PR:P09077; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003339; Expressed in prothoracic segment and 20 other tissues.
DR ExpressionAtlas; P09077; baseline and differential.
DR Genevisible; P09077; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0007494; P:midgut development; TAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007432; P:salivary gland boundary specification; TAS:FlyBase.
DR GO; GO:0045498; P:sex comb development; IMP:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017995; Homeobox_antennapedia.
DR InterPro; IPR001827; Homeobox_Antennapedia_CS.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00025; ANTENNAPEDIA.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00032; ANTENNAPEDIA; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..417
FT /note="Homeotic protein Sex combs reduced"
FT /id="PRO_0000200264"
FT DNA_BIND 324..383
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 24..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 169..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 303..308
FT /note="Antp-type hexapeptide"
FT COMPBIAS 181..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..300
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 216
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 118
FT /note="Q -> L (in Ref. 7; AAA19240)"
FT /evidence="ECO:0000305"
FT CONFLICT 133..135
FT /note="HAH -> LAL (in Ref. 7; AAA19240)"
FT /evidence="ECO:0000305"
FT CONFLICT 141
FT /note="A -> R (in Ref. 7; AAA19240)"
FT /evidence="ECO:0000305"
FT CONFLICT 258
FT /note="G -> R (in Ref. 3; CAA54348)"
FT /evidence="ECO:0000305"
FT CONFLICT 317..323
FT /note="TVNANGE -> YCESNGQ (in Ref. 8; CAA28857)"
FT /evidence="ECO:0000305"
FT CONFLICT 374
FT /note="N -> D (in Ref. 7; AAA19240)"
FT /evidence="ECO:0000305"
FT CONFLICT 379
FT /note="W -> L (in Ref. 8; CAA28857)"
FT /evidence="ECO:0000305"
FT CONFLICT 393
FT /note="Y -> C (in Ref. 7; AAA19240)"
FT /evidence="ECO:0000305"
FT CONFLICT 402
FT /note="Y -> C (in Ref. 7; AAA19240)"
FT /evidence="ECO:0000305"
FT HELIX 306..308
FT /evidence="ECO:0007829|PDB:2R5Y"
FT HELIX 333..343
FT /evidence="ECO:0007829|PDB:2R5Y"
FT HELIX 351..360
FT /evidence="ECO:0007829|PDB:2R5Y"
FT HELIX 365..380
FT /evidence="ECO:0007829|PDB:2R5Y"
SQ SEQUENCE 417 AA; 44263 MW; 8A3A58A60E0B32BE CRC64;
MDPDCFAMSS YQFVNSLASC YPQQMNPQQN HPGAGNSSAG GSGGGAGGSG GVVPSGGTNG
GQGSAGAATP GANDYFPAAA AYTPNLYPNT PQAHYANQAA YGGQGNPDMV DYTQLQPQRL
LLQQQQQQQQ QQHAHAAAAV AAQQQQQLAQ QQHPQQQQQQ QQANISCKYA NDPVTPGGSG
GGGVSGSNNN NNSANSNNNN SQSLASPQDL STRDISPKLS PSSVVESVAR SLNKGVLGGS
LAAAAAAAGL NNNHSGSGVS GGPGNVNVPM HSPGGGDSDS ESDSGNEAGS SQNSGNGKKN
PPQIYPWMKR VHLGTSTVNA NGETKRQRTS YTRYQTLELE KEFHFNRYLT RRRRIEIAHA
LCLTERQIKI WFQNRRMKWK KEHKMASMNI VPYHMGPYGH PYHQFDIHPS QFAHLSA
