ID   SCTA_DICDI              Reviewed;         174 AA.
AC   O77257; Q54XL2;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Secreted protein A {ECO:0000303|PubMed:27187592};
DE   AltName: Full=Protein gp17 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=p17; Synonyms=sctA {ECO:0000303|PubMed:27187592};
GN   ORFNames=DDB_G0278725;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=AX2;
RA   Ryckewaert J.J., Louwagie M., Journet A.M., Paintrand I., Satre M.,
RA   Garin J.;
RT   "Characterization of Dictyostelium discoideum secreted vesicles
RT   accumulating a 17 kDa protein.";
RL   Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   SUBCELLULAR LOCATION, AND DISULFIDE BOND.
RX   PubMed=27187592; DOI=10.1371/journal.pone.0154875;
RA   Sabra A., Leiba J., Mas L., Louwagie M., Coute Y., Journet A., Cosson P.,
RA   Aubry L.;
RT   "Pycnosomes: condensed endosomal structures secreted by Dictyostelium
RT   amoebae.";
RL   PLoS ONE 11:E0154875-E0154875(2016).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=27340834; DOI=10.1371/journal.pone.0158270;
RA   Denoncourt A.M., Paquet V.E., Sedighi A., Charette S.J.;
RT   "Identification of proteins associated with multilamellar bodies produced
RT   by Dictyostelium discoideum.";
RL   PLoS ONE 11:E0158270-E0158270(2016).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27187592,
CC       ECO:0000269|PubMed:27340834}. Extracellular vesicle
CC       {ECO:0000269|PubMed:27187592}. Note=Most abundant protein present in
CC       secreted pycnosomes, which consist of membranous materials that are
CC       continuously secreted (PubMed:27187592). Also found in multilamellar
CC       bodies, which consist of structures of lysosomal origin composed of
CC       multiple concentric membrane layers (PubMed:27340834).
CC       {ECO:0000269|PubMed:27187592, ECO:0000269|PubMed:27340834}.
CC   -!- PTM: Probably contains disulfide bonds. {ECO:0000305|PubMed:27187592}.
CC   -!- SIMILARITY: Belongs to the Sct family. {ECO:0000305}.
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DR   EMBL; Y17042; CAA76600.1; -; mRNA.
DR   EMBL; AAFI02000024; EAL67964.1; -; Genomic_DNA.
DR   RefSeq; XP_647791.1; XM_642699.1.
DR   AlphaFoldDB; O77257; -.
DR   SMR; O77257; -.
DR   STRING; 44689.DDB0215014; -.
DR   PaxDb; O77257; -.
DR   ABCD; O77257; 1 sequenced antibody.
DR   EnsemblProtists; EAL67964; EAL67964; DDB_G0278725.
DR   GeneID; 8621750; -.
DR   KEGG; ddi:DDB_G0278725; -.
DR   dictyBase; DDB_G0278725; p17.
DR   eggNOG; ENOG502RHU4; Eukaryota.
DR   HOGENOM; CLU_1542883_0_0_1; -.
DR   InParanoid; O77257; -.
DR   OMA; CGITQFV; -.
DR   PhylomeDB; O77257; -.
DR   PRO; PR:O77257; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR   GO; GO:0042599; C:lamellar body; IDA:dictyBase.
DR   GO; GO:0032195; C:post-lysosomal vacuole; IDA:dictyBase.
PE   1: Evidence at protein level;
KW   Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..174
FT                   /note="Secreted protein A"
FT                   /id="PRO_5000147294"
FT   CARBOHYD        156
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   174 AA;  18579 MW;  06E2FBC62A8D614D CRC64;
     MRLLITLFAI FALFNCSLAN TATNVGEFLI GFAQGVEITL NSNSQACLSG VTSTFDEFQT
     AFQLIDSGFK SKSINQVKTG ITDLGLAIQQ VPVDYDACGI TQFVEEIEEI ASKLSSGVDG
     IVDVILKEAV EIWEHKNDLS ADFKTAIADW KSSNYNGSGV AVGSIIGDLI QGAK