ID   SCTG_SHISO              Reviewed;         142 AA.
AC   P0A1X3; Q06083; Q9AFS1;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Type 3 secretion system pilotin {ECO:0000305};
DE   AltName: Full=Lipoprotein MxiM;
DE   Flags: Precursor;
GN   Name=mxiM {ECO:0000303|Ref.1};
GN   Synonyms=sctG {ECO:0000250|UniProtKB:P0A1X2};
OS   Shigella sonnei.
OG   Plasmid pINV.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=624;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HW383;
RA   Arakawa E., Kato J., Ito K., Watanabe H.;
RT   "Comparison and high conservation of nucleotide sequences of spa-mxi
RT   regions between S.sonnei and S.flexneri -- identification of a new gene
RT   coding plausible membrane protein.";
RL   Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the synthesis of the type III secretion system
CC       (T3SS), also called injectisome, which is used to inject bacterial
CC       effector proteins into eukaryotic host cells. Pilot protein that is
CC       required for the proper localization of the secretin MxiD/SctC in the
CC       outer membrane. Also influences both MxiD/SctC multimerization and
CC       stability. {ECO:0000250|UniProtKB:P0A1X2}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000250|UniProtKB:P0A1X2}; Lipid-anchor {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the MxiM family. {ECO:0000305}.
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DR   EMBL; D50601; BAA09152.1; -; Genomic_DNA.
DR   RefSeq; WP_001346200.1; NZ_UIQD01000016.1.
DR   AlphaFoldDB; P0A1X3; -.
DR   BMRB; P0A1X3; -.
DR   SMR; P0A1X3; -.
DR   DrugBank; DB04199; 1-Monohexanoyl-2-Hydroxy-Sn-Glycero-3-Phosphate.
DR   OMA; GERQMIR; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.40.128.230; -; 1.
DR   InterPro; IPR021546; MxiM.
DR   InterPro; IPR038517; MxiM_sf.
DR   Pfam; PF11441; MxiM; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal;
KW   Virulence.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           24..142
FT                   /note="Type 3 secretion system pilotin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT                   /id="PRO_0000018226"
FT   LIPID           24
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           24
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   142 AA;  15853 MW;  20D30F76A3EF9B16 CRC64;
     MIRHGSNKLK IFILSILLLT LSGCALKSSS NSEKEWHIVP VSKDYFSIPN DLLWSFNTTN
     KSINVYSKCI SGKAVYSFNA GKFMGNFNVK EVDGCFMDAQ KIAIDKLFSM LKDGVVLKGN
     KINDTILIEK DGEVKLKLIR GI