ID   SCTL1_SALTY             Reviewed;         226 AA.
AC   P0CL45; P40823; P58654;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-MAR-2011, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=SPI-1 type 3 secretion system stator protein {ECO:0000305};
DE            Short=T3SS-1 stator protein {ECO:0000305};
DE   AltName: Full=Oxygen-regulated invasion protein OrgB;
GN   Name=sctL1 {ECO:0000303|PubMed:9618447};
GN   Synonyms=orgB {ECO:0000303|PubMed:10816487}; OrderedLocusNames=STM2869;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [2]
RP   REVIEW, AND NOMENCLATURE.
RX   PubMed=9618447; DOI=10.1128/mmbr.62.2.379-433.1998;
RA   Hueck C.J.;
RT   "Type III protein secretion systems in bacterial pathogens of animals and
RT   plants.";
RL   Microbiol. Mol. Biol. Rev. 62:379-433(1998).
RN   [3]
RP   FUNCTION.
RC   STRAIN=SL1344;
RX   PubMed=10816487; DOI=10.1128/iai.68.6.3368-3376.2000;
RA   Klein J.R., Fahlen T.F., Jones B.D.;
RT   "Transcriptional organization and function of invasion genes within
RT   Salmonella enterica serovar Typhimurium pathogenicity island 1, including
RT   the prgH, prgI, prgJ, prgK, orgA, orgB, and orgC genes.";
RL   Infect. Immun. 68:3368-3376(2000).
RN   [4]
RP   INTERACTION WITH INVC/SCTN1.
RC   STRAIN=SL1344;
RX   PubMed=15060043; DOI=10.1128/jb.186.8.2402-2412.2004;
RA   Akeda Y., Galan J.E.;
RT   "Genetic analysis of the Salmonella enterica type III secretion-associated
RT   ATPase InvC defines discrete functional domains.";
RL   J. Bacteriol. 186:2402-2412(2004).
RN   [5]
RP   FUNCTION, SUBUNIT, INTERACTION WITH INVC/SCTN1, SUBCELLULAR LOCATION, AND
RP   CRYO-ELECTRON TOMOGRAPHY.
RX   PubMed=28283062; DOI=10.1016/j.cell.2017.02.022;
RA   Hu B., Lara-Tejero M., Kong Q., Galan J.E., Liu J.;
RT   "In situ molecular architecture of the Salmonella type III secretion
RT   machine.";
RL   Cell 168:1065-1074(2017).
RN   [6]
RP   REVIEW, AND SUBUNIT.
RX   PubMed=30107569; DOI=10.1093/femsle/fny201;
RA   Wagner S., Grin I., Malmsheimer S., Singh N., Torres-Vargas C.E.,
RA   Westerhausen S.;
RT   "Bacterial type III secretion systems: a complex device for the delivery of
RT   bacterial effector proteins into eukaryotic host cells.";
RL   FEMS Microbiol. Lett. 365:0-0(2018).
RN   [7]
RP   SUBUNIT, AND INTERACTION WITH INVC/SCTN1.
RX   PubMed=31288030; DOI=10.1016/j.jmb.2019.07.004;
RA   Bernal I., Boernicke J., Heidemann J., Svergun D., Horstmann J.A.,
RA   Erhardt M., Tuukkanen A., Uetrecht C., Kolbe M.;
RT   "Molecular organization of soluble type III secretion system sorting
RT   platform complexes.";
RL   J. Mol. Biol. 431:3787-3803(2019).
RN   [8] {ECO:0007744|PDB:4YX7, ECO:0007744|PDB:4YXA}
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1-30 IN COMPLEXES WITH SPAO/SCTQ,
RP   DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 17-ILE--ILE-19.
RX   PubMed=25994170; DOI=10.1038/ncomms8125;
RA   Notti R.Q., Bhattacharya S., Lilic M., Stebbins C.E.;
RT   "A common assembly module in injectisome and flagellar type III secretion
RT   sorting platforms.";
RL   Nat. Commun. 6:7125-7125(2015).
CC   -!- FUNCTION: Component of the type III secretion system (T3SS), also
CC       called injectisome, which is used to inject bacterial effector proteins
CC       into eukaryotic host cells (PubMed:28283062). Acts as a regulator of
CC       the InvC/SctN1 ATPase activity (By similarity). Required for invasion
CC       and secretion (PubMed:10816487). {ECO:0000250|UniProtKB:Q99PY0,
CC       ECO:0000269|PubMed:10816487, ECO:0000269|PubMed:28283062}.
CC   -!- SUBUNIT: The core secretion machinery of the T3SS is composed of
CC       approximately 20 different proteins, including cytoplasmic components,
CC       a base, an export apparatus and a needle (PubMed:30107569,
CC       PubMed:28283062). This subunit is part of the cytosolic complex
CC       (PubMed:28283062, PubMed:31288030). Interacts directly with InvC/SctN1
CC       (T3SS-1 ATPase) and SpaO/SctQ (the major sorting platform component)
CC       (PubMed:15060043, PubMed:25994170, PubMed:28283062, PubMed:31288030).
CC       Forms homodimers (PubMed:31288030). {ECO:0000269|PubMed:15060043,
CC       ECO:0000269|PubMed:25994170, ECO:0000269|PubMed:28283062,
CC       ECO:0000269|PubMed:30107569, ECO:0000269|PubMed:31288030}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:28283062}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the gene disrupts proper SpaO/SctQ
CC       localization. {ECO:0000269|PubMed:25994170}.
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DR   EMBL; AE006468; AAL21749.1; -; Genomic_DNA.
DR   RefSeq; NP_461790.1; NC_003197.2.
DR   RefSeq; WP_000916654.1; NC_003197.2.
DR   PDB; 4YX7; X-ray; 2.00 A; C/F=1-30.
DR   PDB; 4YXA; X-ray; 2.35 A; C/F=1-30.
DR   PDBsum; 4YX7; -.
DR   PDBsum; 4YXA; -.
DR   AlphaFoldDB; P0CL45; -.
DR   SASBDB; P0CL45; -.
DR   SMR; P0CL45; -.
DR   STRING; 99287.STM2869; -.
DR   PaxDb; P0CL45; -.
DR   DNASU; 1254392; -.
DR   EnsemblBacteria; AAL21749; AAL21749; STM2869.
DR   GeneID; 1254392; -.
DR   KEGG; stm:STM2869; -.
DR   PATRIC; fig|99287.12.peg.3025; -.
DR   HOGENOM; CLU_106719_0_0_6; -.
DR   OMA; DYICEWK; -.
DR   PhylomeDB; P0CL45; -.
DR   BioCyc; SENT99287:STM2869-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Protein transport; Reference proteome; Transport;
KW   Virulence.
FT   CHAIN           1..226
FT                   /note="SPI-1 type 3 secretion system stator protein"
FT                   /id="PRO_0000058083"
FT   MUTAGEN         17..19
FT                   /note="ILI->DDD: Disrupts interaction with SpaO/SctQ."
FT                   /evidence="ECO:0000269|PubMed:25994170"
FT   STRAND          2..5
FT                   /evidence="ECO:0007829|PDB:4YX7"
FT   TURN            15..17
FT                   /evidence="ECO:0007829|PDB:4YX7"
FT   HELIX           21..24
FT                   /evidence="ECO:0007829|PDB:4YX7"
SQ   SEQUENCE   226 AA;  26462 MW;  7414148597F28AA7 CRC64;
     MLKNIPIPSP LSPVEGILIK RKTLERYFSI ERLEQQAHQR AKRILREAEE EAKTLRMYAY
     QEGYEQGMID ALQQVAAYLT DNQTMAWKWM EKIQIYAREL FSAAVDHPET LLTVLDEWLR
     DFDKPEGQLF LTLPVNAKKD HQKLMVLLME NWPGTFNLKY HQEQRFIMSC GDQIAEFSPE
     QFVETAVGVI KHHLDELPQD CRTISDNAIN ALIDEWKTKT QAEVIR