SCTL_SHIFL
ID SCTL_SHIFL Reviewed; 231 AA.
AC Q99PY0; A0A2G3FQJ0; A0A2S4MRM1; Q8VSH2;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Type 3 secretion system stator protein {ECO:0000305};
DE Short=T3SS stator protein {ECO:0000305};
DE AltName: Full=MxiN spoke protein {ECO:0000303|PubMed:31699894};
GN Name=sctL {ECO:0000303|PubMed:9618447};
GN Synonyms=mxiN {ECO:0000312|EMBL:AAL72324.2};
GN ORFNames=SF_p0141 {ECO:0000312|EMBL:AAL72324.2};
OS Shigella flexneri.
OG Plasmid pCP301 {ECO:0000312|EMBL:AAL72324.2,
OG ECO:0000312|Proteomes:UP000001006}.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP REVIEW, AND NOMENCLATURE.
RX PubMed=9618447; DOI=10.1128/mmbr.62.2.379-433.1998;
RA Hueck C.J.;
RT "Type III protein secretion systems in bacterial pathogens of animals and
RT plants.";
RL Microbiol. Mol. Biol. Rev. 62:379-433(1998).
RN [3]
RP FUNCTION, SUBUNIT, INTERACTION WITH SPA47/SCTN AND SPA33/SCTQ, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=M90T / Serotype 5a;
RX PubMed=12864857; DOI=10.1046/j.1365-2958.2003.03590.x;
RA Jouihri N., Sory M.P., Page A.L., Gounon P., Parsot C., Allaoui A.;
RT "MxiK and MxiN interact with the Spa47 ATPase and are required for transit
RT of the needle components MxiH and MxiI, but not of Ipa proteins, through
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 49:755-767(2003).
RN [4]
RP SUBUNIT, INTERACTION WITH SPA47/SCTN, AND SUBCELLULAR LOCATION.
RC STRAIN=M90T / Serotype 5a;
RX PubMed=18657109; DOI=10.1111/j.1574-6968.2008.01284.x;
RA Johnson S., Blocker A.;
RT "Characterization of soluble complexes of the Shigella flexneri type III
RT secretion system ATPase.";
RL FEMS Microbiol. Lett. 286:274-278(2008).
RN [5]
RP FUNCTION, SUBUNIT, INTERACTION WITH SPA47/SCTN, AND SUBCELLULAR LOCATION.
RC STRAIN=M90T / Serotype 5a;
RX PubMed=25583506; DOI=10.1073/pnas.1411610112;
RA Hu B., Morado D.R., Margolin W., Rohde J.R., Arizmendi O., Picking W.L.,
RA Picking W.D., Liu J.;
RT "Visualization of the type III secretion sorting platform of Shigella
RT flexneri.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:1047-1052(2015).
RN [6]
RP FUNCTION, SUBUNIT, AND INTERACTION WITH SPA47/SCTN.
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=29595954; DOI=10.1021/acs.biochem.8b00070;
RA Case H.B., Dickenson N.E.;
RT "MxiN differentially regulates monomeric and oligomeric species of the
RT Shigella type three secretion system ATPase Spa47.";
RL Biochemistry 57:2266-2277(2018).
RN [7]
RP REVIEW, AND SUBUNIT.
RX PubMed=30107569; DOI=10.1093/femsle/fny201;
RA Wagner S., Grin I., Malmsheimer S., Singh N., Torres-Vargas C.E.,
RA Westerhausen S.;
RT "Bacterial type III secretion systems: a complex device for the delivery of
RT bacterial effector proteins into eukaryotic host cells.";
RL FEMS Microbiol. Lett. 365:0-0(2018).
RN [8]
RP SUBUNIT, AND INTERACTION WITH SPA33/SCTQ.
RX PubMed=31699894; DOI=10.1074/jbc.ra119.009125;
RA Tachiyama S., Chang Y., Muthuramalingam M., Hu B., Barta M.L.,
RA Picking W.L., Liu J., Picking W.D.;
RT "The cytoplasmic domain of MxiG interacts with MxiK and directs assembly of
RT the sorting platform in the Shigella type III secretion system.";
RL J. Biol. Chem. 294:19184-19196(2019).
CC -!- FUNCTION: Component of the type III secretion system (T3SS), also
CC called injectisome, which is used to inject bacterial effector proteins
CC into eukaryotic host cells (PubMed:12864857, PubMed:29595954). Acts as
CC a regulator of the Spa47/SctN ATPase activity (PubMed:29595954). It
CC down-regulates the ATPase activity of the oligomeric Spa47/SctN, while
CC it up-regulates the activity of the monomeric form (PubMed:29595954).
CC Important for translocation of MxiH/SctF, the major needle component
CC (PubMed:25583506). {ECO:0000269|PubMed:12864857,
CC ECO:0000269|PubMed:25583506, ECO:0000269|PubMed:29595954}.
CC -!- SUBUNIT: The core secretion machinery of the T3SS is composed of
CC approximately 20 different proteins, including cytoplasmic components,
CC a base, an export apparatus and a needle (PubMed:30107569,
CC PubMed:25583506, PubMed:31699894). This subunit is part of the
CC cytosolic complex (PubMed:18657109, PubMed:25583506, PubMed:31699894).
CC Interacts directly with Spa47/SctN (T3SS ATPase) and Spa33/SctQ (the
CC major sorting platform component) (PubMed:12864857, PubMed:18657109,
CC PubMed:25583506, PubMed:29595954, PubMed:31699894). Homodimer in
CC solution (PubMed:29595954). {ECO:0000269|PubMed:12864857,
CC ECO:0000269|PubMed:18657109, ECO:0000269|PubMed:25583506,
CC ECO:0000269|PubMed:29595954, ECO:0000269|PubMed:30107569,
CC ECO:0000269|PubMed:31699894}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18657109,
CC ECO:0000269|PubMed:25583506, ECO:0000305|PubMed:12864857}.
CC -!- DISRUPTION PHENOTYPE: Inactivation of the gene abolishes the ability of
CC bacteria to assemble a complete type III secretion apparatus (T3SA) and
CC therefore to secrete the translocon proteins IpaB/SctE and IpaC/SctB,
CC and decreases the amounts of the needle filament proteins MxiH/SctF and
CC MxiI/SctI (PubMed:12864857). Mutant is unable to invade HeLa cells
CC (PubMed:12864857). {ECO:0000269|PubMed:12864857}.
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DR EMBL; AF386526; AAL72324.2; -; Genomic_DNA.
DR RefSeq; NP_085304.1; NC_002698.1.
DR RefSeq; NP_858274.2; NC_004851.1.
DR RefSeq; WP_010921671.1; NZ_WPGS01000043.1.
DR RefSeq; YP_009062498.1; NC_024996.1.
DR AlphaFoldDB; Q99PY0; -.
DR SMR; Q99PY0; -.
DR STRING; 198214.CP0141; -.
DR EnsemblBacteria; AAL72324; AAL72324; SF_p0141.
DR GeneID; 1238027; -.
DR KEGG; sfl:CP0141; -.
DR PATRIC; fig|198214.7.peg.5391; -.
DR HOGENOM; CLU_1199123_0_0_6; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Cytoplasm; Plasmid; Protein transport; Reference proteome; Transport;
KW Virulence.
FT CHAIN 1..231
FT /note="Type 3 secretion system stator protein"
FT /id="PRO_0000452699"
SQ SEQUENCE 231 AA; 26820 MW; F8E90E95D4ABFC83 CRC64;
MKVCNMQKGT LPVSRHHAYD GVVIKRIEKE LCKTIKDRDT ESKKKAICVI KEATKKAESL
RIDAVCDGYQ IGIQTAFEHI IDYICEWKLK QNENRRNIED YITSLLSENL HDERIISTLL
EQWLSSLRNT VTELKVVLPK CNLALRKKLE LDLHKYRSDV KIILKYSEGN NYIFCSGNQV
VEFSPQDVIS GVKIELAEKL TKNDKKYFKE LAHKKLRQIA EDLLKENPVN D
