ID   SCTL_YEREN              Reviewed;         223 AA.
AC   Q01253; P97147; P97148;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Type 3 secretion system stator protein {ECO:0000305};
DE            Short=T3SS stator protein {ECO:0000305};
DE   AltName: Full=Yop proteins translocation protein L;
GN   Name=sctL {ECO:0000303|PubMed:9618447};
GN   Synonyms=yscL {ECO:0000303|PubMed:1860816};
OS   Yersinia enterocolitica.
OG   Plasmid pYV.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=439-80 / Serotype O:9;
RX   PubMed=1860816; DOI=10.1128/jb.173.16.4994-5009.1991;
RA   Michiels T., Vanooteghem J.-C., de Rouvroit C., China B., Gustin A.,
RA   Boudry P., Cornelis G.R.;
RT   "Analysis of virC, an operon involved in the secretion of Yop proteins by
RT   Yersinia enterocolitica.";
RL   J. Bacteriol. 173:4994-5009(1991).
RN   [2]
RP   REVIEW, AND NOMENCLATURE.
RX   PubMed=9618447; DOI=10.1128/mmbr.62.2.379-433.1998;
RA   Hueck C.J.;
RT   "Type III protein secretion systems in bacterial pathogens of animals and
RT   plants.";
RL   Microbiol. Mol. Biol. Rev. 62:379-433(1998).
RN   [3]
RP   FUNCTION, SUBUNIT, INTERACTION WITH YSCN/SCTN AND YSCQ/SCTQ, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=W22703 / Serotype O:9 / Biotype 2;
RX   PubMed=16672607; DOI=10.1128/jb.188.10.3525-3534.2006;
RA   Blaylock B., Riordan K.E., Missiakas D.M., Schneewind O.;
RT   "Characterization of the Yersinia enterocolitica type III secretion ATPase
RT   YscN and its regulator, YscL.";
RL   J. Bacteriol. 188:3525-3534(2006).
RN   [4]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX   PubMed=20453832; DOI=10.1038/emboj.2010.84;
RA   Diepold A., Amstutz M., Abel S., Sorg I., Jenal U., Cornelis G.R.;
RT   "Deciphering the assembly of the Yersinia type III secretion injectisome.";
RL   EMBO J. 29:1928-1940(2010).
RN   [5]
RP   REVIEW, AND SUBUNIT.
RX   PubMed=30107569; DOI=10.1093/femsle/fny201;
RA   Wagner S., Grin I., Malmsheimer S., Singh N., Torres-Vargas C.E.,
RA   Westerhausen S.;
RT   "Bacterial type III secretion systems: a complex device for the delivery of
RT   bacterial effector proteins into eukaryotic host cells.";
RL   FEMS Microbiol. Lett. 365:0-0(2018).
CC   -!- FUNCTION: Component of the type III secretion system (T3SS), also
CC       called injectisome, which is used to inject bacterial effector proteins
CC       into eukaryotic host cells (PubMed:16672607, PubMed:20453832). Acts as
CC       a regulator of the YscN/SctN ATPase activity (PubMed:16672607).
CC       Overexpression of YscL/SctL abolishes type III secretion and down-
CC       regulates the expression of secretion apparatus components
CC       (PubMed:16672607). {ECO:0000269|PubMed:16672607,
CC       ECO:0000269|PubMed:20453832}.
CC   -!- SUBUNIT: The core secretion machinery of the T3SS is composed of
CC       approximately 20 different proteins, including cytoplasmic components,
CC       a base, an export apparatus and a needle (PubMed:30107569,
CC       PubMed:20453832). This subunit is part of the cytosolic complex
CC       (PubMed:20453832). Interacts directly with YscN/SctN (T3SS ATPase) and
CC       YscQ/SctQ (the major sorting platform component) (PubMed:16672607).
CC       Forms homodimers (PubMed:16672607). {ECO:0000269|PubMed:16672607,
CC       ECO:0000269|PubMed:20453832, ECO:0000269|PubMed:30107569}.
CC   -!- INTERACTION:
CC       Q01253; Q56844: yscU; Xeno; NbExp=3; IntAct=EBI-6502609, EBI-6403263;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:20453832}.
CC   -!- INDUCTION: At 37 degrees Celsius in the absence of calcium.
CC       {ECO:0000269|PubMed:1860816}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant does not support type III
CC       secretion. {ECO:0000269|PubMed:16672607}.
CC   -!- SIMILARITY: Belongs to the SctL stator family. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-12 is the initiator.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC37030.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAC37031.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M74011; AAC37029.1; -; Genomic_DNA.
DR   EMBL; M74011; AAC37030.1; ALT_INIT; Genomic_DNA.
DR   EMBL; M74011; AAC37031.1; ALT_INIT; Genomic_DNA.
DR   PIR; C40049; C40049.
DR   RefSeq; WP_010891233.1; NZ_KN150737.1.
DR   AlphaFoldDB; Q01253; -.
DR   SMR; Q01253; -.
DR   IntAct; Q01253; 2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030254; P:protein secretion by the type III secretion system; IEA:InterPro.
DR   InterPro; IPR018035; Flagellar_FliH/T3SS_HrpE.
DR   InterPro; IPR012842; T3SS_SctL/SctL2.
DR   Pfam; PF02108; FliH; 1.
DR   TIGRFAMs; TIGR02499; HrpE_YscL_not; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Plasmid; Protein transport; Transport; Virulence.
FT   CHAIN           1..223
FT                   /note="Type 3 secretion system stator protein"
FT                   /id="PRO_0000066492"
SQ   SEQUENCE   223 AA;  24977 MW;  4B64F681A5819D47 CRC64;
     MSQTCQTGYA YMQPFVQIIP SNLSLACGLR ILRAEDYQSS LTTEELISAA KQDAEKILAD
     AQEVYEQQKQ LGWQAGMDEA RTLQATLIHE TQLQCQQFYR HVEQQMSEVV LLAVRKILND
     YDQVDMTLQV VREALALVSN QKQVVVRVNP DQAGTIREQI AKVHKDFPEI SYLEVTADAR
     LDQGGCILET EVGIIDASID GQIEALSRAI STTLGQMKVT EEE