ID   SCTL_YERPS              Reviewed;         221 AA.
AC   P69977; Q00928; Q663H8;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Type 3 secretion system stator protein {ECO:0000305};
DE            Short=T3SS stator protein {ECO:0000305};
DE   AltName: Full=Low calcium response locus protein KC;
DE   AltName: Full=Yop proteins translocation protein L;
GN   Name=sctL {ECO:0000303|PubMed:9618447}; Synonyms=lcrKC, yscL;
GN   OrderedLocusNames=pYV0088;
OS   Yersinia pseudotuberculosis serotype I (strain IP32953).
OG   Plasmid pIB1, and Plasmid pYV.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=273123;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=YPIII / Serotype O:3; PLASMID=pIB1;
RX   PubMed=1577700; DOI=10.1128/jb.174.10.3355-3363.1992;
RA   Rimpilaeinen M., Forsberg A., Wolf-Watz H.;
RT   "A novel protein, LcrQ, involved in the low-calcium response of Yersinia
RT   pseudotuberculosis shows extensive homology to YopH.";
RL   J. Bacteriol. 174:3355-3363(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IP32953; PLASMID=pYV;
RX   PubMed=15358858; DOI=10.1073/pnas.0404012101;
RA   Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O.,
RA   Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L.,
RA   Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C.,
RA   Simonet M., Chenal-Francisque V., Souza B., Dacheux D., Elliott J.M.,
RA   Derbise A., Hauser L.J., Garcia E.;
RT   "Insights into the evolution of Yersinia pestis through whole-genome
RT   comparison with Yersinia pseudotuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004).
RN   [3]
RP   REVIEW, AND NOMENCLATURE.
RX   PubMed=9618447; DOI=10.1128/mmbr.62.2.379-433.1998;
RA   Hueck C.J.;
RT   "Type III protein secretion systems in bacterial pathogens of animals and
RT   plants.";
RL   Microbiol. Mol. Biol. Rev. 62:379-433(1998).
RN   [4]
RP   REVIEW, AND SUBUNIT.
RX   PubMed=30107569; DOI=10.1093/femsle/fny201;
RA   Wagner S., Grin I., Malmsheimer S., Singh N., Torres-Vargas C.E.,
RA   Westerhausen S.;
RT   "Bacterial type III secretion systems: a complex device for the delivery of
RT   bacterial effector proteins into eukaryotic host cells.";
RL   FEMS Microbiol. Lett. 365:0-0(2018).
CC   -!- FUNCTION: Component of the type III secretion system (T3SS), also
CC       called injectisome, which is used to inject bacterial effector proteins
CC       into eukaryotic host cells (By similarity). Acts as a regulator of the
CC       YscN/SctN ATPase activity (By similarity).
CC       {ECO:0000250|UniProtKB:Q01253}.
CC   -!- SUBUNIT: The core secretion machinery of the T3SS is composed of
CC       approximately 20 different proteins, including cytoplasmic components,
CC       a base, an export apparatus and a needle (PubMed:30107569). This
CC       subunit is part of the cytosolic complex (By similarity). Interacts
CC       directly with YscN/SctN (T3SS ATPase) and YscQ/SctQ (the major sorting
CC       platform component) (By similarity). {ECO:0000250|UniProtKB:Q01253,
CC       ECO:0000269|PubMed:30107569}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q01253}.
CC   -!- INDUCTION: At 37 degrees Celsius in the absence of calcium.
CC   -!- SIMILARITY: Belongs to the SctL stator family. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-12 is the initiator.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAF25431.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M83986; AAA27653.1; -; Genomic_DNA.
DR   EMBL; BX936399; CAF25431.1; ALT_INIT; Genomic_DNA.
DR   PIR; A41877; A41877.
DR   RefSeq; WP_010981371.1; NC_006153.2.
DR   AlphaFoldDB; P69977; -.
DR   SMR; P69977; -.
DR   EnsemblBacteria; CAF25431; CAF25431; pYV0088.
DR   GeneID; 66841087; -.
DR   KEGG; yps:pYV0088; -.
DR   OMA; CIIETEA; -.
DR   Proteomes; UP000001011; Plasmid pYV.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030254; P:protein secretion by the type III secretion system; IEA:InterPro.
DR   InterPro; IPR018035; Flagellar_FliH/T3SS_HrpE.
DR   InterPro; IPR012842; T3SS_SctL/SctL2.
DR   Pfam; PF02108; FliH; 1.
DR   TIGRFAMs; TIGR02499; HrpE_YscL_not; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Plasmid; Protein transport; Transport; Virulence.
FT   CHAIN           1..221
FT                   /note="Type 3 secretion system stator protein"
FT                   /id="PRO_0000066494"
SQ   SEQUENCE   221 AA;  24645 MW;  7181CEEA9D2C54BD CRC64;
     MSQTCQTGYA YMQPFVQIIP SNLSLACGLR ILRAEDYQSS LTTEELISAA KQDAEKILAD
     AQEVYEQQKQ LGWQAGMDEA RTLQATLIHE TQLQCQQFYR HVEQQMSEVV LLAVRKILND
     YDQVAMTLQV VREALALVSN QKQVVVRVNP DQAGAIREQI AKVHKDFPEI SYLEVTADAR
     LDQGGCILET EVGIIDASID GQIEALSRAI STTLGQMKVT E