SCW10_YEAST
ID SCW10_YEAST Reviewed; 389 AA.
AC Q04951; D6W0D2;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Probable family 17 glucosidase SCW10;
DE EC=3.2.1.-;
DE AltName: Full=Soluble cell wall protein 10;
DE Flags: Precursor;
GN Name=SCW10; OrderedLocusNames=YMR305C; ORFNames=YM9952.07C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP PROTEIN SEQUENCE OF 30-39, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 96099 / S288c / SEY6210;
RX PubMed=9748433; DOI=10.1128/jb.180.19.5030-5037.1998;
RA Cappellaro C., Mrsa V., Tanner W.;
RT "New potential cell wall glucanases of Saccharomyces cerevisiae and their
RT involvement in mating.";
RL J. Bacteriol. 180:5030-5037(1998).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Glucanases possibly play a role in cell expansion during
CC growth, in cell-cell fusion during mating, and in spore release during
CC sporulation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:9748433}.
CC -!- PTM: Glycosylated.
CC -!- MISCELLANEOUS: Present with 10500 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR EMBL; Z49212; CAA89138.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10206.1; -; Genomic_DNA.
DR PIR; S53975; S53975.
DR RefSeq; NP_014035.1; NM_001182815.1.
DR AlphaFoldDB; Q04951; -.
DR SMR; Q04951; -.
DR BioGRID; 35485; 50.
DR DIP; DIP-3845N; -.
DR IntAct; Q04951; 1.
DR STRING; 4932.YMR305C; -.
DR CAZy; GH17; Glycoside Hydrolase Family 17.
DR iPTMnet; Q04951; -.
DR MaxQB; Q04951; -.
DR PaxDb; Q04951; -.
DR PRIDE; Q04951; -.
DR EnsemblFungi; YMR305C_mRNA; YMR305C; YMR305C.
DR GeneID; 855352; -.
DR KEGG; sce:YMR305C; -.
DR SGD; S000004921; SCW10.
DR VEuPathDB; FungiDB:YMR305C; -.
DR eggNOG; ENOG502QTKT; Eukaryota.
DR GeneTree; ENSGT00940000176321; -.
DR HOGENOM; CLU_027285_1_0_1; -.
DR InParanoid; Q04951; -.
DR OMA; GTDCDQT; -.
DR BioCyc; YEAST:YMR305C-MON; -.
DR PRO; PR:Q04951; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04951; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IDA:SGD.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
DR GO; GO:0042124; F:1,3-beta-glucanosyltransferase activity; IBA:GO_Central.
DR GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IBA:GO_Central.
DR GO; GO:0015926; F:glucosidase activity; ISS:SGD.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IBA:GO_Central.
DR GO; GO:0000747; P:conjugation with cellular fusion; IGI:SGD.
DR InterPro; IPR000490; Glyco_hydro_17.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF00332; Glyco_hydro_17; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE 1: Evidence at protein level;
KW Cell wall; Cell wall biogenesis/degradation;
KW Cleavage on pair of basic residues; Direct protein sequencing;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT PROPEP 19..29
FT /evidence="ECO:0000269|PubMed:9748433"
FT /id="PRO_0000011901"
FT CHAIN 30..389
FT /note="Probable family 17 glucosidase SCW10"
FT /id="PRO_0000011902"
FT REGION 70..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 326
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:O22317"
FT CARBOHYD 279
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 38..39
FT /note="AQ -> QE (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 389 AA; 40469 MW; 21F394BD41337DE1 CRC64;
MRFSNFLTVS ALLTGALGAP AVRHKHEKRD VVTATVHAQV TVVVSGNSGE TIVPVNENAV
VATTSSTAVA SQATTSTLEP TTSANVVTSQ QQTSTLQSSE AASTVGSSTS SSPSSSSSTS
SSASSSASSS ISASGAKGIT YSPYNDDGSC KSTAQVASDL EQLTGFDNIR LYGVDCSQVE
NVLQAKTSSQ KLFLGIYYVD KIQDAVDTIK SAVESYGSWD DITTVSVGNE LVNGGSATTT
QVGEYVSTAK SALTSAGYTG SVVSVDTFIA VINNPDLCNY SDYMAVNAHA YFDENTAAQD
AGPWVLEQIE RVYTACGGKK DVVITETGWP SKGDTYGEAV PSKANQEAAI SSIKSSCGSS
AYLFTAFNDL WKDDGQYGVE KYWGILSSD
