ID   SCX1_ANUPH              Reviewed;          90 AA.
AC   Q5MJP5; P84207;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Phaiodotoxin;
DE   Flags: Precursor;
GN   Name=phtx {ECO:0000312|EMBL:AAW29437.1};
OS   Anuroctonus phaiodactylus (Mafia scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Chactoidea; Chactidae; Uroctoninae; Anuroctonus.
OX   NCBI_TaxID=246982;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAW29437.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-90, FUNCTION, BIOASSAY,
RP   TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND DISULFIDE
RP   BONDS.
RC   TISSUE=Venom {ECO:0000269|PubMed:15606762};
RX   PubMed=15606762; DOI=10.1111/j.1432-1033.2004.04439.x;
RA   Valdez-Cruz N.A., Batista C.V.F., Zamudio F.Z., Bosmans F., Tytgat J.,
RA   Possani L.D.;
RT   "Phaiodotoxin, a novel structural class of insect-toxin isolated from the
RT   venom of the Mexican scorpion Anuroctonus phaiodactylus.";
RL   Eur. J. Biochem. 271:4753-4761(2004).
CC   -!- FUNCTION: Sodium channel (Nav) specific neurotoxin. Causes impairment
CC       of movement and mild paralysis in crickets at a dose of 0.5 ug per
CC       animal. A dose of 0.8 ug per cricket causes clear flaccid paralysis. A
CC       dose of 1.0 ug per cricket causes death within 2 hours.
CC       {ECO:0000269|PubMed:15606762}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15606762}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:15606762}.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7971; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:15606762};
CC   -!- MISCELLANEOUS: Non-toxic to mice at a dose of 100 ug per 20 g mouse
CC       weight. {ECO:0000305|PubMed:15606762}.
CC   -!- SIMILARITY: Belongs to the long (4 C-C) scorpion toxin superfamily.
CC       Sodium channel inhibitor family. {ECO:0000305}.
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DR   EMBL; AY781122; AAW29437.1; -; mRNA.
DR   AlphaFoldDB; Q5MJP5; -.
DR   GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR002061; Scorpion_toxinL/defensin.
DR   Pfam; PF00537; Toxin_3; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51863; LCN_CSAB; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Secreted; Signal; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000269|PubMed:15606762"
FT   CHAIN           19..90
FT                   /note="Phaiodotoxin"
FT                   /evidence="ECO:0000269|PubMed:15606762"
FT                   /id="PRO_0000035234"
FT   DOMAIN          19..90
FT                   /note="LCN-type CS-alpha/beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        31..56
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210,
FT                   ECO:0000269|PubMed:15606762"
FT   DISULFID        41..68
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210,
FT                   ECO:0000269|PubMed:15606762"
FT   DISULFID        45..70
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210,
FT                   ECO:0000269|PubMed:15606762"
FT   DISULFID        81..89
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210,
FT                   ECO:0000269|PubMed:15606762"
SQ   SEQUENCE   90 AA;  10124 MW;  BB6FD57A8A47F2A6 CRC64;
     MKTIPLLFLL FIYFECDGKF IRHKDESFYE CGQLIGYQQY CVDACQAHGS KEKGYCKGMA
     PFGLPGGCYC PKLPSNRVKM CFGALESKCA