ID   SCX1_BUTMA              Reviewed;          67 AA.
AC   P0DJH8;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 1.
DT   03-AUG-2022, entry version 24.
DE   RecName: Full=Alpha-toxin Bu1;
OS   Buthacus macrocentrus (Turkish scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Buthacus.
OX   NCBI_TaxID=1143368;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-65, FUNCTION, TOXIC DOSE,
RP   IDENTIFICATION BY MASS SPECTROMETRY, AMIDATION AT ARG-65, AND SUBCELLULAR
RP   LOCATION.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=22245624; DOI=10.1016/j.toxicon.2011.12.013;
RA   Caliskan F., Quintero-Hernandez V., Restano-Cassulini R., Batista C.V.,
RA   Zamudio F.Z., Coronas F.I., Possani L.D.;
RT   "Turkish scorpion Buthacus macrocentrus: general characterization of the
RT   venom and description of Bu1, a potent mammalian Na-channel alpha-toxin.";
RL   Toxicon 59:408-415(2012).
CC   -!- FUNCTION: Alpha toxins bind voltage-independently at site-3 of sodium
CC       channels (Nav) and inhibit the inactivation of the activated channels,
CC       thereby blocking neuronal transmission. Since the experiments have been
CC       done on F11 cells (immortalized cell line derived from rat DRG neurons
CC       mainly expressing Nav1.3/SCN3A, but also Nav1.7/SCN9A and
CC       Nav1.2/SCN2A), it is supposed to act on these channels. The slow of
CC       inactivation process is partially reversible. Is lethal to mice.
CC       {ECO:0000269|PubMed:22245624}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22245624}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:22245624}.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000250|UniProtKB:P0DQN8}.
CC   -!- TOXIC DOSE: 10 ug of this toxin kills a mouse of 20g body weight within
CC       20 minutes after intraperitoneal injection.
CC       {ECO:0000269|PubMed:22245624}.
CC   -!- SIMILARITY: Belongs to the long (4 C-C) scorpion toxin superfamily.
CC       Sodium channel inhibitor family. Alpha subfamily. {ECO:0000305}.
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DR   EMBL; JQ038136; AFG28506.1; -; mRNA.
DR   AlphaFoldDB; P0DJH8; -.
DR   SMR; P0DJH8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:InterPro.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR018218; Scorpion_toxinL.
DR   InterPro; IPR002061; Scorpion_toxinL/defensin.
DR   Pfam; PF00537; Toxin_3; 1.
DR   PRINTS; PR00285; SCORPNTOXIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51863; LCN_CSAB; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..65
FT                   /note="Alpha-toxin Bu1"
FT                   /id="PRO_0000417436"
FT   DOMAIN          3..65
FT                   /note="LCN-type CS-alpha/beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   MOD_RES         65
FT                   /note="Arginine amide"
FT                   /evidence="ECO:0000269|PubMed:22245624"
FT   DISULFID        13..64
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        17..37
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        23..47
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        27..49
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
SQ   SEQUENCE   67 AA;  7485 MW;  B2FBC35EFCF8C70B CRC64;
     GVRDAYIADD KNCVYTCAKN SYCNTECTKN GAESGYCQWL GKYGNGCWCI KLPDKVPIRI
     PGRCRGR