SCX4_CENNO
ID SCX4_CENNO Reviewed; 87 AA.
AC P45662; Q6V4Y1;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Beta-toxin Cn4;
DE Short=Toxin 4;
DE AltName: Full=Toxin CngtV;
DE AltName: Full=Toxin II-10;
DE Flags: Precursor;
OS Centruroides noxius (Mexican scorpion).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Buthida; Buthoidea; Buthidae; Centruroides.
OX NCBI_TaxID=6878;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-85, AND AMIDATION AT
RP ASN-85.
RC TISSUE=Venom, and Venom gland;
RX PubMed=8462674; DOI=10.1016/0014-5793(93)81654-i;
RA Vazquez A., Becerril B., Martin B.M., Zamudio F.Z., Bolivar F.,
RA Possani L.D.;
RT "Primary structure determination and cloning of the cDNA encoding toxin 4
RT of the scorpion Centruroides noxius Hoffmann.";
RL FEBS Lett. 320:43-46(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=8390386; DOI=10.1016/0378-1119(93)90559-l;
RA Becerril B., Vazquez A., Garcia C., Corona M., Bolivar F., Possani L.D.;
RT "Cloning and characterization of cDNAs that code for Na(+)-channel-blocking
RT toxins of the scorpion Centruroides noxius Hoffmann.";
RL Gene 128:165-171(1993).
RN [3]
RP PARTIAL PROTEIN SEQUENCE.
RA Possani L.D., Dent M.A.R., Martin B.M., Maelicke A., Svendsen I.;
RT "The amino terminal sequence of several toxins from the venom of the
RT Mexican scorpion Centruroides noxius Hoffmann.";
RL Carlsberg Res. Commun. 46:207-214(1981).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-84.
RA Zhu S.;
RT "Alignment of beta-toxin nucleotide sequences.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION ON SODIUM CHANNEL ACTIVATION.
RX PubMed=6099409;
RA Carbone E., Prestipino G., Franciolini F., Dent M.A.R., Possani L.D.;
RT "Selective modification of the squid axon Na currents by Centruroides
RT noxius toxin II-10.";
RL J. Physiol. (Paris) 79:179-184(1984).
CC -!- FUNCTION: Beta toxins bind voltage-independently at site-4 of sodium
CC channels (Nav) and shift the voltage of activation toward more negative
CC potentials thereby affecting sodium channel activation and promoting
CC spontaneous and repetitive firing. This toxin affects the activation
CC mechanism of sodium channels of squid axon. It also competes with Cn2
CC in rat brain synaptosomes. Is lethal to mice.
CC {ECO:0000269|PubMed:6099409}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the long (4 C-C) scorpion toxin superfamily.
CC Sodium channel inhibitor family. Beta subfamily. {ECO:0000305}.
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DR EMBL; L05063; AAA28288.1; -; mRNA.
DR EMBL; AY351309; AAR08044.1; -; Genomic_DNA.
DR PIR; JN0672; JN0672.
DR AlphaFoldDB; P45662; -.
DR SMR; P45662; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR018218; Scorpion_toxinL.
DR PRINTS; PR00285; SCORPNTOXIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51863; LCN_CSAB; 1.
PE 1: Evidence at protein level;
KW Amidation; Direct protein sequencing; Disulfide bond;
KW Ion channel impairing toxin; Neurotoxin; Secreted; Signal; Toxin;
KW Voltage-gated sodium channel impairing toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|PubMed:8462674"
FT CHAIN 20..85
FT /note="Beta-toxin Cn4"
FT /id="PRO_0000035281"
FT DOMAIN 20..85
FT /note="LCN-type CS-alpha/beta"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT MOD_RES 85
FT /note="Asparagine amide"
FT /evidence="ECO:0000269|PubMed:8462674"
FT DISULFID 31..84
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 35..60
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 44..65
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 48..67
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT CONFLICT 27
FT /note="Missing (in Ref. 4; AAR08044)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 87 AA; 9788 MW; 76BF746651C0C376 CRC64;
MNSLLMITAC LALVGTVWAK EGYLVNSYTG CKYECFKLGD NDYCLRECKQ QYGKGAGGYC
YAFGCWCTHL YEQAVVWPLK NKTCNGK
