SCX8_CENEL
ID SCX8_CENEL Reviewed; 64 AA.
AC P0CH40;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 1.
DT 03-AUG-2022, entry version 24.
DE RecName: Full=Beta-toxin CeII8;
OS Centruroides elegans (Bark scorpion).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Buthida; Buthoidea; Buthidae; Centruroides.
OX NCBI_TaxID=217897;
RN [1]
RP PROTEIN SEQUENCE, MASS SPECTROMETRY, TOXIN TARGET, AND FUNCTION.
RC TISSUE=Venom;
RX PubMed=20600228; DOI=10.1016/j.toxicon.2010.06.008;
RA Vandendriessche T., Olamendi-Portugal T., Zamudio F.Z., Possani L.D.,
RA Tytgat J.;
RT "Isolation and characterization of two novel scorpion toxins: The alpha-
RT toxin-like CeII8, specific for Na(v)1.7 channels and the classical anti-
RT mammalian CeII9, specific for Na(v)1.4 channels.";
RL Toxicon 56:613-623(2010).
CC -!- FUNCTION: Beta toxins bind at site-4 of sodium channels and shift the
CC voltage of activation toward more negative potentials thereby affecting
CC sodium channel activation and promoting spontaneous and repetitive
CC firing. Has a selective effect toward Nav1.7/SCN9A which plays a role
CC in pain mechanisms, and especially in the development of inflammatory
CC pain. Shows no lethality toward mice. {ECO:0000269|PubMed:20600228}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=7450.0; Method=Unknown;
CC Evidence={ECO:0000269|PubMed:20600228};
CC -!- MISCELLANEOUS: The protein sequence seems to be related to the alpha-
CC toxins, however experimental data show that it has a clear beta-toxin
CC activity. {ECO:0000305|PubMed:20600228}.
CC -!- SIMILARITY: Belongs to the long (4 C-C) scorpion toxin superfamily.
CC Sodium channel inhibitor family. Alpha subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P0CH40; -.
DR SMR; P0CH40; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR018218; Scorpion_toxinL.
DR InterPro; IPR002061; Scorpion_toxinL/defensin.
DR Pfam; PF00537; Toxin_3; 1.
DR PRINTS; PR00285; SCORPNTOXIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51863; LCN_CSAB; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Neurotoxin; Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT CHAIN 1..64
FT /note="Beta-toxin CeII8"
FT /id="PRO_0000398149"
FT DOMAIN 2..61
FT /note="LCN-type CS-alpha/beta"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 12..60
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 16..36
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 22..43
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 26..45
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
SQ SEQUENCE 64 AA; 7459 MW; A10BAB2727124CEB CRC64;
KKDGYPVNME ECRYNCWKNA YCDKLCKEKK GQSGYCYGWN LSCWCIGLPD NTNTKMNPFC
QTAD