SCX9_BUTOC
ID SCX9_BUTOC Reviewed; 70 AA.
AC P04099;
DT 01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2017, sequence version 2.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Alpha-toxin Bot9 {ECO:0000303|PubMed:27528512};
DE AltName: Full=BotIX {ECO:0000303|PubMed:27528512};
DE AltName: Full=Neurotoxin 9 {ECO:0000305};
DE AltName: Full=Neurotoxin IX {ECO:0000305};
OS Buthus occitanus tunetanus (Common European scorpion) (Buthus tunetanus).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Buthida; Buthoidea; Buthidae; Buthus.
OX NCBI_TaxID=6871;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP TOXIC DOSE.
RC TISSUE=Venom {ECO:0000303|PubMed:27528512};
RX PubMed=27528512; DOI=10.1002/1873-3468.12357;
RA Martin-Eauclaire M.F., Salvatierra J., Bosmans F., Bougis P.E.;
RT "The scorpion toxin Bot IX is a potent member of the alpha-like family and
RT has a unique N-terminal sequence extension.";
RL FEBS Lett. 590:3221-3232(2016).
RN [2]
RP PROTEIN SEQUENCE OF 1-35, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom {ECO:0000303|PubMed:6729843};
RX PubMed=6729843; DOI=10.1016/0041-0101(84)90028-x;
RA Martin M.-F., Rochat H.;
RT "Purification of thirteen toxins active on mice from the venom of the North
RT African scorpion Buthus occitanus tunetanus.";
RL Toxicon 22:279-291(1984).
CC -!- FUNCTION: Alpha toxins bind voltage-independently at site-3 of sodium
CC channels (Nav) and inhibit the inactivation of the activated channels,
CC thereby blocking neuronal transmission. This toxin is active against
CC rat Nav1.2/SCN2A and B.germanica Nav1. {ECO:0000269|PubMed:27528512}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27528512,
CC ECO:0000269|PubMed:6729843}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:6729843}.
CC -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=7985.61; Mass_error=0.11; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:27528512};
CC -!- TOXIC DOSE: LD(50) is 100 ng/kg by subcutaneous injection in mice.
CC {ECO:0000269|PubMed:27528512}.
CC -!- SIMILARITY: Belongs to the long (4 C-C) scorpion toxin superfamily.
CC Sodium channel inhibitor family. Alpha subfamily. {ECO:0000305}.
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DR PIR; A05136; A05136.
DR AlphaFoldDB; P04099; -.
DR SMR; P04099; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR018218; Scorpion_toxinL.
DR InterPro; IPR002061; Scorpion_toxinL/defensin.
DR Pfam; PF00537; Toxin_3; 1.
DR PRINTS; PR00285; SCORPNTOXIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51863; LCN_CSAB; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Neurotoxin; Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT CHAIN 1..70
FT /note="Alpha-toxin Bot9"
FT /evidence="ECO:0000269|PubMed:27528512"
FT /id="PRO_0000066737"
FT DOMAIN 6..69
FT /note="LCN-type CS-alpha/beta"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 16..68
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 20..40
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 26..50
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 30..52
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT CONFLICT 23
FT /note="N -> D (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 32..33
FT /note="Missing (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 70 AA; 7998 MW; E24EB04C32040548 CRC64;
AEIKVRDGYI VYPNNCVYHC GLNPYCNDLC TKNGAKSGYC QWLTKWGNAC YCYALPEKVP
IKDPSYKCYS
