SCXB_MESMA
ID SCXB_MESMA Reviewed; 84 AA.
AC Q9N682;
DT 19-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Neurotoxin BmK-M11;
DE Short=BmK11;
DE Short=Bmk M11;
DE Short=BmkM11;
DE AltName: Full=Alpha-neurotoxin TX14;
DE Short=BmalphaTX14 {ECO:0000303|PubMed:22343065};
DE AltName: Full=BmK M4';
DE AltName: Full=BmK-XI;
DE Flags: Precursor;
OS Mesobuthus martensii (Manchurian scorpion) (Buthus martensii).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX NCBI_TaxID=34649;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=10728828; DOI=10.1016/s0041-0101(99)00192-0;
RA Zeng X.-C., Li W.-X., Zhu S.-Y., Peng F., Jiang D.-H., Yang F.-H.,
RA Wu K.-L.;
RT "Cloning and characterization of the cDNA sequences of two venom peptides
RT from Chinese scorpion Buthus martensii Karsch (BmK).";
RL Toxicon 38:893-899(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=10858508; DOI=10.1016/s0041-0101(00)00081-7;
RA Zhu S.-Y., Li W.-X., Zeng X.-C., Liu H., Jiang D.-H., Mao X.;
RT "Nine novel precursors of Buthus martensii scorpion alpha-toxin
RT homologues.";
RL Toxicon 38:1653-1661(2000).
RN [3]
RP FUNCTION.
RX PubMed=22343065; DOI=10.1016/j.pep.2012.02.001;
RA Dai H., Yin S., Li T., Cao Z., Ji Y., Wu Y., Li W.;
RT "Recombinant expression, purification, and characterization of scorpion
RT toxin BmalphaTX14.";
RL Protein Expr. Purif. 82:325-331(2012).
CC -!- FUNCTION: Alpha toxins bind voltage-independently at site-3 of sodium
CC channels (Nav) and inhibit the inactivation of the activated channels,
CC thereby blocking neuronal transmission. This recombinant toxin
CC selectively inhibits the fast inactivation of mNav1.4/SCN4A
CC (EC(50)=82.3 nM) (tested in HEK293 cells).
CC {ECO:0000269|PubMed:22343065}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: Does not inhibit rNav1.2/SCN2A (EC(50) is >30000 nM)
CC channels. {ECO:0000269|PubMed:22343065}.
CC -!- SIMILARITY: Belongs to the long (4 C-C) scorpion toxin superfamily.
CC Sodium channel inhibitor family. Alpha subfamily. {ECO:0000305}.
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DR EMBL; AF114025; AAF34872.1; -; mRNA.
DR EMBL; AF156169; AAF29462.1; -; mRNA.
DR EMBL; AF156595; AAK06897.1; -; mRNA.
DR PIR; JE0143; JE0143.
DR AlphaFoldDB; Q9N682; -.
DR SMR; Q9N682; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR018218; Scorpion_toxinL.
DR InterPro; IPR002061; Scorpion_toxinL/defensin.
DR Pfam; PF00537; Toxin_3; 1.
DR PRINTS; PR00285; SCORPNTOXIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51863; LCN_CSAB; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Ion channel impairing toxin; Neurotoxin; Secreted; Signal;
KW Toxin; Voltage-gated sodium channel impairing toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000250"
FT CHAIN 20..83
FT /note="Neurotoxin BmK-M11"
FT /id="PRO_0000035242"
FT PROPEP 84
FT /note="Removed by a carboxypeptidase"
FT /evidence="ECO:0000305"
FT /id="PRO_0000035243"
FT DOMAIN 21..83
FT /note="LCN-type CS-alpha/beta"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 31..82
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 35..55
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 41..65
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT DISULFID 45..67
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
SQ SEQUENCE 84 AA; 9308 MW; C08EA4859A30B7B6 CRC64;
MNYLVMISFA LLLMTGVESV RDAYIAKPEN CVYHCATNEG CNKLCTDNGA ESGYCQWGGK
YGNACWCIKL PDDVPIRVPG KCHR
