ID   SCXIT_ANDMA             Reviewed;          65 AA.
AC   P0DPT3;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   10-APR-2019, sequence version 1.
DT   03-AUG-2022, entry version 9.
DE   RecName: Full=Beta-toxin Am IT {ECO:0000303|PubMed:26109302};
DE            Short=Am IT {ECO:0000303|PubMed:26109302};
OS   Androctonus mauritanicus mauritanicus (Scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Androctonus.
OX   NCBI_TaxID=6860;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TOXIC DOSE, PYROGLUTAMATE
RP   FORMATION AT GLU-1, AMIDATION AT SER-65, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=26109302;
RA   Oukkache N., ElJaoudi R., Chgoury F., Rocha M.T., Sabatier J.M.;
RT   "Characterization of Am IT, an anti-insect beta-toxin isolated from the
RT   venom of scorpion Androctonus mauretanicus.";
RL   Sheng Li Xue Bao 67:295-304(2015).
CC   -!- FUNCTION: Has a toxic effect on insects and mammals. On German
CC       cockroach larvae, it provokes contraction, paralysis and lethality
CC       (PubMed:26109302). Intracerebroventricular injection into mice causes
CC       severe neurotoxic symptoms (PubMed:26109302). It fully competes with
CC       the binding of the iodinated Css4 (AC P60266) on rat brain
CC       synaptosomes, with moderate affinity and in a concentration-dependent
CC       manner (EC(50)=25 nM). It may act on both site 3 and site 4 of voltage-
CC       gated sodium channels. {ECO:0000269|PubMed:26109302}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26109302}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:26109302}.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7831.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:26109302};
CC   -!- MASS SPECTROMETRY: Mass=7803.8; Method=MALDI; Note=with pyrrolidone
CC       carboxylic acid (Glu).; Evidence={ECO:0000269|PubMed:26109302};
CC   -!- TOXIC DOSE: LD(50) is 80 ng/100 mg of body weight cockroach larvae
CC       (B.germanica). {ECO:0000269|PubMed:26109302}.
CC   -!- MISCELLANEOUS: This toxin does not inhibit the binding of iodinated
CC       Css2 (AC P08900) and iodinated Ts1 (AC P15226) to their specific
CC       antibodies. {ECO:0000269|PubMed:26109302}.
CC   -!- SIMILARITY: Belongs to the long (4 C-C) scorpion toxin superfamily.
CC       Sodium channel inhibitor family. {ECO:0000305}.
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DR   AlphaFoldDB; P0DPT3; -.
DR   SMR; P0DPT3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:InterPro.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR018218; Scorpion_toxinL.
DR   InterPro; IPR002061; Scorpion_toxinL/defensin.
DR   Pfam; PF00537; Toxin_3; 1.
DR   PRINTS; PR00285; SCORPNTOXIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51863; LCN_CSAB; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Pyrrolidone carboxylic acid;
KW   Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..65
FT                   /note="Beta-toxin Am IT"
FT                   /evidence="ECO:0000269|PubMed:26109302"
FT                   /id="PRO_0000446449"
FT   DOMAIN          1..64
FT                   /note="LCN-type CS-alpha/beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid (Glu); partial"
FT                   /evidence="ECO:0000269|PubMed:26109302"
FT   MOD_RES         65
FT                   /note="Serine amide"
FT                   /evidence="ECO:0000269|PubMed:26109302"
FT   DISULFID        12..63
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        16..38
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        23..45
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        27..47
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
SQ   SEQUENCE   65 AA;  7699 MW;  42653CD18D843E10 CRC64;
     EHGYLLDKYT GCKVWCVINN ESCNGECNKR RGGYYGYCYF WKLACYCQGA RKSELWNYKT
     NKCKS