SCX_MOUSE
ID SCX_MOUSE Reviewed; 207 AA.
AC Q64124;
DT 25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Basic helix-loop-helix transcription factor scleraxis;
GN Name=Scx;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7743923; DOI=10.1242/dev.121.4.1099;
RA Cserjesi P., Brown D., Ligon K.L., Lyons G.E., Copeland N.G., Gilbert D.J.,
RA Jenkins N.A., Olson E.N.;
RT "Scleraxis: a basic helix-loop-helix protein that prefigures skeletal
RT formation during mouse embryogenesis.";
RL Development 121:1099-1110(1995).
RN [2]
RP CHARACTERIZATION.
RX PubMed=10477299; DOI=10.1242/dev.126.19.4317;
RA Brown D., Wagner D., Li X.-Q., Richardson J.A., Olson E.N.;
RT "Dual role of the basic helix-loop-helix transcription factor scleraxis in
RT mesoderm formation and chondrogenesis during mouse embryogenesis.";
RL Development 126:4317-4329(1999).
CC -!- FUNCTION: Plays an early essential role in mesoderm formation, as well
CC as a later role in formation of somite-derived chondrogenic lineages.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Dimerizes and binds the E-box consensus sequence with E12.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed in mesenchymal precursors of cartilage
CC and in connective tissue. Highly expressed in tendons in the limb,
CC tongue and diaphragm and in cartilage of the bronchi.
CC -!- DEVELOPMENTAL STAGE: At 6.0-6.5 dpc, expressed throughout the egg
CC cylinder with highest expression in the epiblast. At 9.5-10.5 dpc,
CC expressed in the lateral sclerotome and in mesenchymal cells of the
CC limb buds and body wall. At 11.0 dpc, expressed in a metameric pattern
CC extending along the length of the embryo. By 11.5 dpc, expression in
CC the developing vertebrae and invertebral disks is extended caudally.
CC High expression was seen in precursors of the ribs and bones of the
CC limbs. Expression in progenitors of the ribs and the axial and
CC appendicular skeleton becomes down-regulated when ossification is
CC initiated.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; S78079; AAB34266.1; -; mRNA.
DR CCDS; CCDS27571.1; -.
DR RefSeq; NP_942588.1; NM_198885.3.
DR AlphaFoldDB; Q64124; -.
DR SMR; Q64124; -.
DR BioGRID; 203115; 1.
DR STRING; 10090.ENSMUSP00000043668; -.
DR iPTMnet; Q64124; -.
DR PhosphoSitePlus; Q64124; -.
DR PaxDb; Q64124; -.
DR PRIDE; Q64124; -.
DR Antibodypedia; 72507; 88 antibodies from 19 providers.
DR DNASU; 20289; -.
DR Ensembl; ENSMUST00000043089; ENSMUSP00000043668; ENSMUSG00000034161.
DR GeneID; 20289; -.
DR KEGG; mmu:20289; -.
DR UCSC; uc007wkg.2; mouse.
DR CTD; 642658; -.
DR MGI; MGI:102934; Scx.
DR VEuPathDB; HostDB:ENSMUSG00000034161; -.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000161897; -.
DR HOGENOM; CLU_115077_0_0_1; -.
DR InParanoid; Q64124; -.
DR OMA; PPKEYRQ; -.
DR OrthoDB; 1511100at2759; -.
DR PhylomeDB; Q64124; -.
DR TreeFam; TF315153; -.
DR BioGRID-ORCS; 20289; 5 hits in 74 CRISPR screens.
DR PRO; PR:Q64124; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q64124; protein.
DR Bgee; ENSMUSG00000034161; Expressed in facial mesenchyme and 153 other tissues.
DR ExpressionAtlas; Q64124; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IDA:UniProtKB.
DR GO; GO:0043425; F:bHLH transcription factor binding; IPI:UniProtKB.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0070888; F:E-box binding; IDA:UniProtKB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0030509; P:BMP signaling pathway; IMP:UniProtKB.
DR GO; GO:0051216; P:cartilage development; NAS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IMP:UniProtKB.
DR GO; GO:0071773; P:cellular response to BMP stimulus; IEP:UniProtKB.
DR GO; GO:0071260; P:cellular response to mechanical stimulus; IEP:UniProtKB.
DR GO; GO:0071560; P:cellular response to transforming growth factor beta stimulus; IEP:UniProtKB.
DR GO; GO:0002062; P:chondrocyte differentiation; IMP:UniProtKB.
DR GO; GO:0030199; P:collagen fibril organization; IMP:UniProtKB.
DR GO; GO:0035993; P:deltoid tuberosity development; IMP:UniProtKB.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0048706; P:embryonic skeletal system development; IEP:UniProtKB.
DR GO; GO:0001958; P:endochondral ossification; IMP:UniProtKB.
DR GO; GO:0060325; P:face morphogenesis; IEP:UniProtKB.
DR GO; GO:0003188; P:heart valve formation; IMP:UniProtKB.
DR GO; GO:0003179; P:heart valve morphogenesis; IMP:UniProtKB.
DR GO; GO:0001707; P:mesoderm formation; IMP:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0061036; P:positive regulation of cartilage development; IMP:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:UniProtKB.
DR GO; GO:0032967; P:positive regulation of collagen biosynthetic process; IDA:UniProtKB.
DR GO; GO:2000543; P:positive regulation of gastrulation; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0061035; P:regulation of cartilage development; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0061056; P:sclerotome development; IEP:UniProtKB.
DR GO; GO:0060008; P:Sertoli cell differentiation; ISS:UniProtKB.
DR GO; GO:0035914; P:skeletal muscle cell differentiation; IMP:MGI.
DR GO; GO:0035990; P:tendon cell differentiation; IMP:UniProtKB.
DR GO; GO:0035989; P:tendon development; IMP:UniProtKB.
DR GO; GO:0035992; P:tendon formation; IMP:UniProtKB.
DR GO; GO:0001894; P:tissue homeostasis; IMP:UniProtKB.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..207
FT /note="Basic helix-loop-helix transcription factor
FT scleraxis"
FT /id="PRO_0000127436"
FT DOMAIN 78..130
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..44
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..88
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..174
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 207 AA; 22239 MW; 8340052200A85EF9 CRC64;
MSFAMLRSAP PPGRYLYPEV SPLSEDEDRG SESSGSDEKP CRVHAARCGL QGARRRAGGR
RAAGSGPGPG GRPGREPRQR HTANARERDR TNSVNTAFTA LRTLIPTEPA DRKLSKIETL
RLASSYISHL GNVLLVGEAC GDGQPCHSGP AFFHSGRAGS PLPPPPPPPP LARDGGENTQ
PKQICTFCLS NQRKLSKDRD RKTAIRS
