BETT_ECO57
ID BETT_ECO57 Reviewed; 677 AA.
AC P0ABD0; P17447;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=High-affinity choline transport protein {ECO:0000250|UniProtKB:P0ABC9};
GN Name=betT; OrderedLocusNames=Z0401, ECs0360;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: High-affinity uptake of choline driven by a proton-motive
CC force. {ECO:0000250|UniProtKB:P0ABC9}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=choline(in) + H(+)(in) = choline(out) + H(+)(out);
CC Xref=Rhea:RHEA:28843, ChEBI:CHEBI:15354, ChEBI:CHEBI:15378;
CC Evidence={ECO:0000250|UniProtKB:P0ABC9};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28845;
CC Evidence={ECO:0000250|UniProtKB:P0ABC9};
CC -!- PATHWAY: Amine and polyamine biosynthesis; betaine biosynthesis via
CC choline pathway. {ECO:0000250|UniProtKB:P0ABC9}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0ABC9}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: By osmotic stress. Choline is required for full expression.
CC {ECO:0000250|UniProtKB:P0ABC9}.
CC -!- SIMILARITY: Belongs to the BCCT transporter (TC 2.A.15) family.
CC {ECO:0000305}.
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DR EMBL; AE005174; AAG54656.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB33783.1; -; Genomic_DNA.
DR PIR; D85524; D85524.
DR PIR; H90673; H90673.
DR RefSeq; NP_308387.1; NC_002695.1.
DR RefSeq; WP_000131044.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0ABD0; -.
DR SMR; P0ABD0; -.
DR STRING; 155864.EDL933_0363; -.
DR EnsemblBacteria; AAG54656; AAG54656; Z0401.
DR EnsemblBacteria; BAB33783; BAB33783; ECs_0360.
DR GeneID; 66671386; -.
DR GeneID; 914461; -.
DR KEGG; ece:Z0401; -.
DR KEGG; ecs:ECs_0360; -.
DR PATRIC; fig|386585.9.peg.452; -.
DR eggNOG; COG1292; Bacteria.
DR HOGENOM; CLU_010118_3_1_6; -.
DR OMA; WAMYALM; -.
DR UniPathway; UPA00529; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0019285; P:glycine betaine biosynthetic process from choline; IEA:UniProtKB-UniPathway.
DR GO; GO:0071705; P:nitrogen compound transport; IEA:InterPro.
DR InterPro; IPR018093; BCCT_CS.
DR InterPro; IPR000060; BCCT_transptr.
DR PANTHER; PTHR30047; PTHR30047; 1.
DR Pfam; PF02028; BCCT; 1.
DR TIGRFAMs; TIGR00842; bcct; 1.
DR PROSITE; PS01303; BCCT; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Stress response; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..677
FT /note="High-affinity choline transport protein"
FT /id="PRO_0000201485"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 677 AA; 75842 MW; 76062FCE877D0A55 CRC64;
MTDLSHSREK DKINPVVFYT SAGLILLFSL TTILFRDFSA LWIGRTLDWV SKTFGWYYLL
AATLYIVFVV CIACSRFGSV KLGPEQSKPE FSLLSWAAML FAAGIGIDLM FFSVAEPVTQ
YMQPPEGAGQ TIEAARQAMV WTLFHYGLTG WSMYALMGMA LGYFSYRYNL PLTIRSALYP
IFGKRINGPI GHSVDIAAVI GTIFGIATTL GIGVVQLNYG LSVLFDIPDS MAAKAALIAL
SVIIATISVT SGVDKGIRVL SELNVALALG LILFVLFMGD TSFLLNALVL NVGDYVNRFM
GMTLNSFAFD RPVEWMNNWT LFFWAWWVAW SPFVGLFLAR ISRGRTIRQF VLGTLIIPFT
FTLLWLSVFG NSALYEIIHG GAAFAEEAMV HPERGFYSLL AQYPAFTFSA SVATITGLLF
YVTSADSGAL VLGNFTSQLK DINSDAPGWL RVFWSVAIGL LTLGMLMTNG ISALQNTTVI
MGLPFSFVIF FVMAGLYKSL KVEDYRRESA NRDTAPRPLG LQDRLSWKKR LSRLMNYPGT
RYTKQMMETV CYPAMEEVAQ ELRLRGAYVE LKSLPPEEGQ QLGHLDLLVH MGEEQNFVYQ
IWPQQYSVPG FTYRARSGKS TYYRLETFLL EGSQGNDLMD YSKEQVITDI LDQYERHLNF
IHLHREAPGH SVMFPDA
