ID   SCYG_SCYSE              Reviewed;         126 AA.
AC   Q5D710;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Scygonadin {ECO:0000303|Ref.2, ECO:0000312|EMBL:AAW57403.1};
DE   Flags: Precursor;
OS   Scylla serrata (Mud crab).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC   Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Brachyura;
OC   Eubrachyura; Portunoidea; Portunidae; Scylla.
OX   NCBI_TaxID=6761;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAW57403.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Seminal plasma {ECO:0000312|EMBL:AAW57403.1};
RX   PubMed=17092560; DOI=10.1016/j.molimm.2006.09.036;
RA   Wang K.-J., Huang W.-S., Yang M., Chen H.-Y., Bo J., Li S.-J., Wang G.-Z.;
RT   "A male-specific expression gene, encodes a novel anionic antimicrobial
RT   peptide, scygonadin, in Scylla serrata.";
RL   Mol. Immunol. 44:1961-1968(2007).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 25-44, SUBCELLULAR LOCATION, AND FUNCTION.
RC   TISSUE=Seminal plasma {ECO:0000269|Ref.2};
RX   DOI=10.1016/j.jembe.2006.06.029;
RA   Huang W.-S., Wang K.-J., Yang M., Cai J.J., Li S.-J., Wang G.-Z.;
RT   "Purification and part characterization of a novel antibacterial protein
RT   Scygonadin isolated from the seminal plasma of mud crab Scylla serrata
RT   (forskaall, 1775).";
RL   J. Exp. Mar. Biol. Ecol. 339:37-42(2006).
CC   -!- FUNCTION: Has antibacterial activity against the Gram-positive
CC       bacterium M.luteus with an IC(90) of 125ug/ml. Has weak antibacterial
CC       activity against the Gram-negative bacterium A.hydrophila.
CC       {ECO:0000269|Ref.2}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.2}.
CC   -!- TISSUE SPECIFICITY: Expressed in the ejaculatory ducts of mature males.
CC       Not detected in the ejaculatory ducts of immature males. Not detected
CC       in hepatopancreas, female reproductive tract, eyes, exoskeleton,
CC       subcuticular epithelia, heart, gills, stomach, muscle and hemocytes.
CC       {ECO:0000269|PubMed:17092560}.
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DR   EMBL; AY864802; AAW57403.1; -; mRNA.
DR   EMBL; DQ059303; AAY98913.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5D710; -.
DR   SMR; Q5D710; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   Gene3D; 3.30.160.320; -; 1.
DR   InterPro; IPR024509; Anti-LPS_factor/Scygonadin.
DR   InterPro; IPR038539; Anti-LPS_factor/Scygonadin_sf.
DR   Pfam; PF11630; DUF3254; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000269|Ref.2"
FT   CHAIN           25..126
FT                   /note="Scygonadin"
FT                   /evidence="ECO:0000269|Ref.2"
FT                   /id="PRO_5000094859"
SQ   SEQUENCE   126 AA;  13736 MW;  F8C69D756B9A7CCB CRC64;
     MRSSLLLGLT VVVLLGVIVP PCMAGQALNK LMPKIVSAII YMVGQPNAGV TFLGHQCLVE
     STRQPDGFYT AKMSCASWTH DNPIVGEGRS RVELEALKGS ITNFVQTASN YKKFTIDEVE
     DWIASY