SDA1_DROME
ID SDA1_DROME Reviewed; 712 AA.
AC Q7KKH3;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein SDA1 homolog;
DE AltName: Full=Mystery protein 45A;
GN Name=Mys45A; ORFNames=CG8070;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11691839; DOI=10.1101/gad.927001;
RA Mohr S.E., Dillon S.T., Boswell R.E.;
RT "The RNA-binding protein Tsunagi interacts with Mago Nashi to establish
RT polarity and localize oskar mRNA during Drosophila oogenesis.";
RL Genes Dev. 15:2886-2899(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-234 AND SER-236, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Required for 60S pre-ribosomal subunits export to the
CC cytoplasm. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SDA1 family. {ECO:0000305}.
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DR EMBL; AF173549; AAL29184.1; -; mRNA.
DR EMBL; AE013599; AAF58986.2; -; Genomic_DNA.
DR EMBL; AY070560; AAL48031.1; -; mRNA.
DR RefSeq; NP_610455.1; NM_136611.4.
DR AlphaFoldDB; Q7KKH3; -.
DR SMR; Q7KKH3; -.
DR BioGRID; 61763; 2.
DR IntAct; Q7KKH3; 3.
DR STRING; 7227.FBpp0087709; -.
DR iPTMnet; Q7KKH3; -.
DR PaxDb; Q7KKH3; -.
DR PRIDE; Q7KKH3; -.
DR DNASU; 35925; -.
DR EnsemblMetazoa; FBtr0088628; FBpp0087709; FBgn0033379.
DR GeneID; 35925; -.
DR KEGG; dme:Dmel_CG8070; -.
DR CTD; 35925; -.
DR FlyBase; FBgn0033379; Mys45A.
DR VEuPathDB; VectorBase:FBgn0033379; -.
DR eggNOG; KOG2229; Eukaryota.
DR GeneTree; ENSGT00390000010355; -.
DR HOGENOM; CLU_009161_3_1_1; -.
DR InParanoid; Q7KKH3; -.
DR OMA; AMYKTYK; -.
DR OrthoDB; 426305at2759; -.
DR PhylomeDB; Q7KKH3; -.
DR SignaLink; Q7KKH3; -.
DR BioGRID-ORCS; 35925; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35925; -.
DR PRO; PR:Q7KKH3; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033379; Expressed in eye disc (Drosophila) and 27 other tissues.
DR Genevisible; Q7KKH3; DM.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IBA:GO_Central.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR027312; Sda1.
DR InterPro; IPR007949; SDA1_dom.
DR InterPro; IPR012977; Uncharacterised_NUC130/133_N.
DR PANTHER; PTHR12730; PTHR12730; 1.
DR Pfam; PF08158; NUC130_3NT; 1.
DR Pfam; PF05285; SDA1; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Protein transport; Reference proteome;
KW Ribosome biogenesis; Transport.
FT CHAIN 1..712
FT /note="Protein SDA1 homolog"
FT /id="PRO_0000287488"
FT REGION 488..573
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 662..712
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 515..555
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 556..573
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 662..676
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 677..692
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 234
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 236
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 712 AA; 81984 MW; 5C263D824D6F2753 CRC64;
MVRPNNNQLP ENLPQLQNLI KRDPESYSDE FHIQYQHFLS LLEVFALNPS EENKSLDDIV
MFVAQVAQCY PAVCEEFPKR LSDLLKNYAT VLDPAMRNCF VKALILLRNK NLVPALDILE
LFFQLLRCPD KNLRTFLQTH IVTDIKNMNA KHKDMKLNSS LQAFMYSMLK DANPKAAKMS
ADIMIELYKK NIWNDSKTVN VIATVGCFSK VTKVLVTSLK FFLGHDEEDE EEDTDSENEV
DLKGALMANR VNKKTKKRTK QLAQIKKQAV KAQKKKKNAP AFNFSGIHLV HNPQGMAEGL
FKQLQATNER FEVKLMHLDV ISRLIGIHDL FLFGFYPYIT RFLQPHQRQV TRVLQFAAQA
SHELVPGDII EPILKTIANN FITERNSSDV MAIGLNATRE ICMRCPLAMG EDLLQDLAMY
KTYKEKSVMM AARSLITLYR EQLPALLHKK DRGRQTEAQA ERKVRAYGER EVHDTVLGAE
ALLKDSKTID IESEDDTDSN DGEWVNVAHS DGEGGGADDD EEDEDEDEDD DDEDEDEENS
NDEENEDEDN SDEGVESGEE SAKAKKEKKD MRILNQKEAA QELALTRIFT DEDFKRINAA
NLKKTVTSAR KRPLEQDRAE FVKLNSIEMI YKKRKHDKES RLETVQAGRQ DRERFGWKDG
RVNEHCSKTN REKRKTKNFG MLRHKARSKV KKSFKDKQQA LRKHLLHQKK MK
