SDC25_YEASX
ID SDC25_YEASX Reviewed; 1253 AA.
AC P0CF32; P14771; Q12037; Q12065; Q6B0T5; Q6WV04;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 25-MAY-2022, entry version 47.
DE RecName: Full=Guanine nucleotide exchange factor SDC25;
GN Name=SDC25; Synonyms=SCD25;
OS Saccharomyces cerevisiae (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=4932;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 200060 / W303;
RX PubMed=1986220; DOI=10.1128/mcb.11.1.202-212.1991;
RA Damak F., Boy-Marcotte E., le Roscouet D., Guilbaud R., Jacquet M.;
RT "SDC25, a CDC25-like gene which contains a RAS-activating domain and is a
RT dispensable gene of Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 11:202-212(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 670-1253.
RC STRAIN=0L136;
RX PubMed=2545538; DOI=10.1016/0378-1119(89)90355-7;
RA Boy-Marcotte E., Damak F., Camonis J., Garreau H., Jacquet M.;
RT "The C-terminal part of a gene partially homologous to CDC 25 gene
RT suppresses the cdc25-5 mutation in Saccharomyces cerevisiae.";
RL Gene 77:21-30(1989).
RN [3]
RP FUNCTION.
RX PubMed=2188363; DOI=10.1126/science.2188363;
RA Crechet J.B., Poullet P., Mistou M.-Y., Parmeggiani A., Camonis J.,
RA Boy-Marcotte E., Damak F., Jacquet M.;
RT "Enhancement of the GDP-GTP exchange of RAS proteins by the carboxyl-
RT terminal domain of SCD25.";
RL Science 248:866-868(1990).
RN [4]
RP FUNCTION.
RX PubMed=2000228;
RA Rey I., Schweighoffer F., Barlat I., Camonis J., Boy-Marcotte E.,
RA Guilbaud R., Jacquet M., Tocque B.;
RT "The COOH-domain of the product of the Saccharomyces cerevisiae SCD25 gene
RT elicits activation of p21-ras proteins in mammalian cells.";
RL Oncogene 6:347-349(1991).
RN [5]
RP FUNCTION.
RX PubMed=8730097; DOI=10.1091/mbc.7.4.529;
RA Boy-Marcotte E., Ikonomi P., Jacquet M.;
RT "SDC25, a dispensable Ras guanine nucleotide exchange factor of
RT Saccharomyces cerevisiae differs from CDC25 by its regulation.";
RL Mol. Biol. Cell 7:529-539(1996).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP.
CC {ECO:0000269|PubMed:2000228, ECO:0000269|PubMed:2188363,
CC ECO:0000269|PubMed:8730097}.
CC -!- MISCELLANEOUS: Suppresses the CDC25-5 mutation in yeast (restores cAMP
CC level) and has similar functions as CDC25.
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DR EMBL; M26647; AAA16565.1; -; Genomic_DNA.
DR PIR; S14177; S14177.
DR AlphaFoldDB; P0CF32; -.
DR SMR; P0CF32; -.
DR IntAct; P0CF32; 4.
DR MINT; P0CF32; -.
DR PRIDE; P0CF32; -.
DR VEuPathDB; FungiDB:YLR310C; -.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR Pfam; PF00018; SH3_1; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SMART; SM00326; SH3; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR SUPFAM; SSF50044; SSF50044; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
DR PROSITE; PS50002; SH3; 1.
PE 4: Predicted;
KW Cell cycle; Cell division; Guanine-nucleotide releasing factor; SH3 domain.
FT CHAIN 1..1253
FT /note="Guanine nucleotide exchange factor SDC25"
FT /id="PRO_0000068893"
FT DOMAIN 26..98
FT /note="SH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT DOMAIN 782..914
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 952..1199
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 624..649
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1202..1253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1215..1237
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1238..1253
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 682..692
FT /note="KSCDDYFDVLK -> NPVMTILMCLN (in strain: 0L136)"
FT VARIANT 740
FT /note="Missing (in strain: 0L136)"
FT VARIANT 783..785
FT /note="PIV -> SNN (in strain: 0L136)"
FT VARIANT 974
FT /note="I -> T (in strain: 0L136)"
FT VARIANT 1048
FT /note="A -> D (in strain: 0L136)"
SQ SEQUENCE 1253 AA; 144980 MW; 2DE2C9EC27E3E60D CRC64;
MSCTASYAGM TTPVKDKEGH GIPCLQPIDV VECTYQYFTK SRNKLSLRVG DLIYVLTKGS
NGWWDGVLIR HSANNNNNNS LILDRGWFPP SFTRSILNEL HGVPDIGNEL EIFQAGLNLK
LELSSNPVIL SLEDFLDCCR DIEFKEQLAW SPTPVHERKG CCELLYYNQD LDVYCRTLPY
LPQNQVETVN DYSSFPAISK IAGKKMPITS SPDLFYLNDC DVVYWYDLTR LVCHYVNLTE
RDLLANEREK FLTSLDLLTA QITYVYMLFR NLRLVEDSFK KTLKKLIYTL SRFSINANIW
FHSTSFEERE AIASQKDPER RSPLLQSILG TFQKFHFLLR LLHFLSNPNE LTILPQLTPR
FFKDSFNTIS WNNPFLRTVF NQHMSMTLPR QMIKAVAGAS GIVAENIDEI PASKQGTFIS
SETSHHSPSA PFQRRRRGTI FSNVSGSSDE SDTIWSKRKK PYPLNEETLS LVRARKKQLD
GKLKQMIKSA NEYLSNTANF SKMLNFEMNF KTYEEVSGTI PIIDILENLD LTIFLNLREL
GDENRVFDED VAIGDEDKEF LKHSLSSLSY ILSDYFNMKQ YFHDVVVKFI IVAQHLTLED
PFVFSPMQND LPTGYYEPMK PSSLNLDNAK DKKNGSQNTD IQEEEDEYEP DPDSLILFHN
LINQDSDFND LKFFNLAHVF KKSCDDYFDV LKLAIEFVNQ LILERENLLN YAARMMKNNI
TELLLRGEEG YGSYDGGETA EKSDTNAVYA DSDTKDNDEW RDSQVKLPRY LQREYDSELI
WGPIVRIKGG SKHALISYLT DNEKKDLFFN ITFLITFRSI FTTTEFLSYL ISQYNLDPPE
DLCFEEYNEW VTKKLIPVKC RVVEIMTTFF KQYWFPGYDE PDLATLNLDY FAQVAIKENI
TGSVELLKEV NQKFKLGNIQ EATAPMKTLD QQICQDHYSG TLYSTTESIL AVDPVLFATQ
LTILEHEIYC EITIFDCLQK IWKNKYTKSY GASPGLNEFI SFANKLTNFI SYSVVKEADK
SKRAKLLSHF IFIAEYCRKF NNFSSMTAII SALYSSPIYR LEKTWQAVIP QTRDLLQSLN
KLMDPKKNFI NYRNELKSLH SAPCVPFFGV YLSDLTFTDS GNPDYLVLEH GLKGVHDEKK
YINFNKRSRL VDILQEIIYF KKTHYDFTKD RTVIECISNS LENIPHIEKQ YQLSLIIEPK
PRKKVVPNSN SNNKSQEKSR DDQTDEGKTS TKKDRFPKFQ LHKTKKKAPK VSK
