ID   SDCG8_DANRE             Reviewed;         701 AA.
AC   B8JK76;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 2.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Serologically defined colon cancer antigen 8 homolog;
GN   Name=Sdccag8; ORFNames=si:dkey-60B15.1;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=20835237; DOI=10.1038/ng.662;
RA   Otto E.A., Hurd T.W., Airik R., Chaki M., Zhou W., Stoetzel C., Patil S.B.,
RA   Levy S., Ghosh A.K., Murga-Zamalloa C.A., van Reeuwijk J., Letteboer S.J.,
RA   Sang L., Giles R.H., Liu Q., Coene K.L., Estrada-Cuzcano A., Collin R.W.,
RA   McLaughlin H.M., Held S., Kasanuki J.M., Ramaswami G., Conte J., Lopez I.,
RA   Washburn J., Macdonald J., Hu J., Yamashita Y., Maher E.R.,
RA   Guay-Woodford L.M., Neumann H.P., Obermuller N., Koenekoop R.K.,
RA   Bergmann C., Bei X., Lewis R.A., Katsanis N., Lopes V., Williams D.S.,
RA   Lyons R.H., Dang C.V., Brito D.A., Dias M.B., Zhang X., Cavalcoli J.D.,
RA   Nurnberg G., Nurnberg P., Pierce E.A., Jackson P.K., Antignac C.,
RA   Saunier S., Roepman R., Dollfus H., Khanna H., Hildebrandt F.;
RT   "Candidate exome capture identifies mutation of SDCCAG8 as the cause of a
RT   retinal-renal ciliopathy.";
RL   Nat. Genet. 42:840-850(2010).
CC   -!- FUNCTION: Plays a role in the establishment of cell polarity and
CC       epithelial lumen formation (PubMed:20835237). Also plays an essential
CC       role in ciliogenesis and subsequent Hedgehog signaling pathway that
CC       requires the presence of intact primary cilia for pathway activation.
CC       Mechanistically, interacts with and mediates RABEP2 centrosomal
CC       localization which is critical for ciliogenesis (By similarity).
CC       {ECO:0000250|UniProtKB:Q86SQ7, ECO:0000269|PubMed:20835237}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome, centriole {ECO:0000250|UniProtKB:Q86SQ7}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC       {ECO:0000250|UniProtKB:Q86SQ7}. Cytoplasm, cytoskeleton, cilium basal
CC       body {ECO:0000250|UniProtKB:Q86SQ7}. Cell junction
CC       {ECO:0000250|UniProtKB:Q86SQ7}. Note=Located at the distal ends of both
CC       centrioles and colocalizes to centrosomes throughout the cell cycle.
CC       {ECO:0000250|UniProtKB:Q86SQ7}.
CC   -!- DISRUPTION PHENOTYPE: Morpholino oligonucleotide-mediated gene
CC       knockdown causes multiple developmental defects, including abnormal
CC       body axis curvature, shortened and broadened tails, kidney cysts at 72
CC       hours post fertilization and hydrocephalus.
CC       {ECO:0000269|PubMed:20835237}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAX14325.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; CT573119; CAX14325.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CU914440; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_017214440.1; XM_017358951.1.
DR   AlphaFoldDB; B8JK76; -.
DR   SMR; B8JK76; -.
DR   STRING; 7955.ENSDARP00000099835; -.
DR   PaxDb; B8JK76; -.
DR   PeptideAtlas; B8JK76; -.
DR   ZFIN; ZDB-GENE-090312-109; cep170aa.
DR   ZFIN; ZDB-GENE-090313-318; sdccag8.
DR   eggNOG; ENOG502R5XE; Eukaryota.
DR   HOGENOM; CLU_024506_0_0_1; -.
DR   InParanoid; B8JK76; -.
DR   PhylomeDB; B8JK76; -.
DR   TreeFam; TF325472; -.
DR   PRO; PR:B8JK76; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW.
DR   GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR   GO; GO:0005813; C:centrosome; IEA:InterPro.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR   GO; GO:0007098; P:centrosome cycle; IEA:InterPro.
DR   GO; GO:0043009; P:chordate embryonic development; IMP:ZFIN.
DR   InterPro; IPR031887; SDCCAG8.
DR   PANTHER; PTHR34343; PTHR34343; 1.
DR   Pfam; PF15964; CCCAP; 1.
PE   3: Inferred from homology;
KW   Cell junction; Cell projection; Cilium biogenesis/degradation; Coiled coil;
KW   Cytoplasm; Cytoskeleton; Reference proteome.
FT   CHAIN           1..701
FT                   /note="Serologically defined colon cancer antigen 8
FT                   homolog"
FT                   /id="PRO_0000407928"
FT   REGION          1..67
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          84..114
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          364..387
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          119..173
FT                   /evidence="ECO:0000255"
FT   COILED          203..258
FT                   /evidence="ECO:0000255"
FT   COILED          323..695
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..25
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        26..67
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   701 AA;  80437 MW;  59198561C8AE7F9C CRC64;
     MKPSLESDEE EELGALQKTL RERANRSIQH LSSMLAGHSS DSETSEPNQQ ELLSSVQQNP
     CSPVQQSEAV SQLRSLLQMK TKDANIQSLS PSRRKLAAKR ATDDGSSSQP GVHNLVPIIN
     NQSEYIQHLE AEVKFCKEEL LEMKQRVRVV IVENEKLHHE LKAKTIEDTL KEYTFVDSTL
     NMEHTAENTL KERLGSVNQA EDHKWRKEME QLKCLYQAQT ETLEAQVVSL KKDLACIQKE
     YEESKERLRH KEAMAVAAGT GQRVSGLCLK CAQHEAVLAE THTNVHVQSI ERLTKERDEL
     MTVLCSLRAS QTDAQQREWG AYQQVKQAVE MAEEANLEKT RALVQCEHFH NELTRQRERL
     ERELASEQDK ISQAREAARS ESKKEREELM QTLSSLSQKV AELQGLLDRG ERDRNSLNSQ
     LEEAYKKLTV QETDSSKMCA ELRFLLSQAQ LKKEEAERDV RDISSKLGRQ LELAEQEVQK
     LGVELSGYRQ RLEEAQRAEG RARAEAAGLA EGLSRAQRQL HLTRQEKEAS ERCCGEDMAA
     LTFQAQRRER ELTQTLQQME AQHEKSVRET DALLSAQNSL IRKLKEECHT LGAKLEELAQ
     SSRAEMEQLS LEREHLQESA EKLRGRCEEM EEQCVQHGRM HQRMKQRLQQ LDQHCQASSQ
     QVLQLLSRQK QLMQERQQLT EDLHSLKSQV HAGKRMDRLA V