SDHF1_MOUSE
ID SDHF1_MOUSE Reviewed; 118 AA.
AC Q3U276; B2RVF8;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Succinate dehydrogenase assembly factor 1, mitochondrial {ECO:0000250|UniProtKB:A6NFY7};
DE Short=SDH assembly factor 1 {ECO:0000250|UniProtKB:A6NFY7};
DE Short=SDHAF1 {ECO:0000250|UniProtKB:A6NFY7};
DE AltName: Full=LYR motif-containing protein 8 {ECO:0000250|UniProtKB:A6NFY7};
GN Name=Sdhaf1 {ECO:0000250|UniProtKB:A6NFY7};
GN Synonyms=Lyrm8 {ECO:0000250|UniProtKB:A6NFY7};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Liver, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays an essential role in the assembly of succinate
CC dehydrogenase (SDH), an enzyme complex (also referred to as respiratory
CC complex II) that is a component of both the tricarboxylic acid (TCA)
CC cycle and the mitochondrial electron transport chain, and which couples
CC the oxidation of succinate to fumarate with the reduction of ubiquinone
CC (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur
CC protein subunit Sdhb of the SDH catalytic dimer, protecting it from the
CC deleterious effects of oxidants. May act together with SDHAF3.
CC Contributes to iron-sulfur cluster incorporation into SDHB by binding
CC to SDHB and recruiting the iron-sulfur transfer complex formed by
CC HSC20, HSPA9 and ISCU through direct binding to HSC20.
CC {ECO:0000250|UniProtKB:A6NFY7}.
CC -!- SUBUNIT: Interacts with SDHB within an SDHA-SDHB subcomplex. Also
CC interacts with the iron-sulfur transfer complex formed by HSC20, HSPA9
CC and ISCU through direct binding to HSC20. Binding of SDHAF1 to SDHB
CC precedes and is necessary for recruitment of the iron-sulfur transfer
CC complex by SDHAF1. {ECO:0000250|UniProtKB:Q3E785}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix
CC {ECO:0000250|UniProtKB:A6NFY7}.
CC -!- SIMILARITY: Belongs to the complex I LYR family. SDHAF1 subfamily.
CC {ECO:0000305}.
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DR EMBL; AK155437; BAE33266.1; -; mRNA.
DR EMBL; BC147173; AAI47174.1; -; mRNA.
DR EMBL; BC147174; AAI47175.1; -; mRNA.
DR CCDS; CCDS39881.1; -.
DR RefSeq; NP_001028312.2; NM_001033140.3.
DR AlphaFoldDB; Q3U276; -.
DR SMR; Q3U276; -.
DR STRING; 10090.ENSMUSP00000096185; -.
DR PhosphoSitePlus; Q3U276; -.
DR EPD; Q3U276; -.
DR MaxQB; Q3U276; -.
DR PaxDb; Q3U276; -.
DR PeptideAtlas; Q3U276; -.
DR PRIDE; Q3U276; -.
DR ProteomicsDB; 253432; -.
DR Antibodypedia; 44606; 199 antibodies from 27 providers.
DR Ensembl; ENSMUST00000098586; ENSMUSP00000096185; ENSMUSG00000074211.
DR GeneID; 68332; -.
DR KEGG; mmu:68332; -.
DR UCSC; uc009ged.1; mouse.
DR CTD; 644096; -.
DR MGI; MGI:1915582; Sdhaf1.
DR VEuPathDB; HostDB:ENSMUSG00000074211; -.
DR eggNOG; KOG4620; Eukaryota.
DR GeneTree; ENSGT00940000157289; -.
DR HOGENOM; CLU_154777_0_1_1; -.
DR InParanoid; Q3U276; -.
DR OMA; FRENARI; -.
DR OrthoDB; 1638205at2759; -.
DR PhylomeDB; Q3U276; -.
DR TreeFam; TF344152; -.
DR BioGRID-ORCS; 68332; 2 hits in 38 CRISPR screens.
DR PRO; PR:Q3U276; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q3U276; protein.
DR Bgee; ENSMUSG00000074211; Expressed in ileal epithelium and 233 other tissues.
DR Genevisible; Q3U276; MM.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0034553; P:mitochondrial respiratory chain complex II assembly; ISS:UniProtKB.
DR CDD; cd20268; Complex1_LYR_SDHAF1_LYRM8; 1.
DR InterPro; IPR008011; Complex1_LYR_dom.
DR InterPro; IPR045295; Complex1_LYR_SDHAF1_LYRM8.
DR Pfam; PF05347; Complex1_LYR; 1.
PE 1: Evidence at protein level;
KW Chaperone; Mitochondrion; Reference proteome; Repeat.
FT CHAIN 1..118
FT /note="Succinate dehydrogenase assembly factor 1,
FT mitochondrial"
FT /id="PRO_0000327917"
FT REGION 53..65
FT /note="Interaction with SDHB"
FT /evidence="ECO:0000250|UniProtKB:A6NFY7"
FT REGION 68..118
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 14..16
FT /note="LYR motif 1; required for interaction with HSC20"
FT /evidence="ECO:0000250|UniProtKB:A6NFY7"
FT MOTIF 53..55
FT /note="LYR motif 2; not required for interaction with
FT HSC20"
FT /evidence="ECO:0000250|UniProtKB:A6NFY7"
SQ SEQUENCE 118 AA; 13142 MW; C1491206E577BCFA CRC64;
MSRPSRLQRQ VLSLYRELLR AGRGTPGAEA RVRAEFRQHA SLPRTDVLRI EYLYRRGRRQ
LQLLRSGHAT AMGTFVRPRG PAEEPGDATA PGTRLDDGGA PKNSCEDTGA RETRSDGR
