ID   SDHF2_KOMPG             Reviewed;         190 AA.
AC   C4R0B5;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Succinate dehydrogenase assembly factor 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03057};
DE            Short=SDH assembly factor 2 {ECO:0000255|HAMAP-Rule:MF_03057};
DE            Short=SDHAF2 {ECO:0000255|HAMAP-Rule:MF_03057};
GN   OrderedLocusNames=PAS_chr2-1_0323;
OS   Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=644223;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GS115 / ATCC 20864;
RX   PubMed=19465926; DOI=10.1038/nbt.1544;
RA   De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S.,
RA   Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT   "Genome sequence of the recombinant protein production host Pichia
RT   pastoris.";
RL   Nat. Biotechnol. 27:561-566(2009).
CC   -!- FUNCTION: Plays an essential role in the assembly of succinate
CC       dehydrogenase (SDH), an enzyme complex (also referred to as respiratory
CC       complex II) that is a component of both the tricarboxylic acid (TCA)
CC       cycle and the mitochondrial electron transport chain, and which couples
CC       the oxidation of succinate to fumarate with the reduction of ubiquinone
CC       (coenzyme Q) to ubiquinol. Required for flavinylation (covalent
CC       attachment of FAD) of the flavoprotein subunit of the SDH catalytic
CC       dimer. {ECO:0000255|HAMAP-Rule:MF_03057}.
CC   -!- SUBUNIT: Interacts with the flavoprotein subunit within the SDH
CC       catalytic dimer. {ECO:0000255|HAMAP-Rule:MF_03057}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000255|HAMAP-
CC       Rule:MF_03057}.
CC   -!- MISCELLANEOUS: This protein may be expected to contain an N-terminal
CC       transit peptide but none has been predicted. {ECO:0000255|HAMAP-
CC       Rule:MF_03057}.
CC   -!- SIMILARITY: Belongs to the SDHAF2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03057}.
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DR   EMBL; FN392320; CAY68939.1; -; Genomic_DNA.
DR   RefSeq; XP_002491219.1; XM_002491174.1.
DR   AlphaFoldDB; C4R0B5; -.
DR   SMR; C4R0B5; -.
DR   STRING; 644223.C4R0B5; -.
DR   EnsemblFungi; CAY68939; CAY68939; PAS_chr2-1_0323.
DR   GeneID; 8199011; -.
DR   KEGG; ppa:PAS_chr2-1_0323; -.
DR   eggNOG; KOG3326; Eukaryota.
DR   HOGENOM; CLU_103054_0_0_1; -.
DR   InParanoid; C4R0B5; -.
DR   OMA; WHSAPYL; -.
DR   Proteomes; UP000000314; Chromosome 2.
DR   GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR   GO; GO:0006121; P:mitochondrial electron transport, succinate to ubiquinone; ISS:UniProtKB.
DR   GO; GO:0034553; P:mitochondrial respiratory chain complex II assembly; IEA:EnsemblFungi.
DR   GO; GO:0017013; P:protein flavinylation; IEA:EnsemblFungi.
DR   GO; GO:0018293; P:protein-FAD linkage; ISS:UniProtKB.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:EnsemblFungi.
DR   Gene3D; 1.10.150.250; -; 1.
DR   HAMAP; MF_03057; SDHAF2; 1.
DR   InterPro; IPR005631; SDH.
DR   InterPro; IPR036714; SDH_sf.
DR   InterPro; IPR028882; SDHAF2.
DR   Pfam; PF03937; Sdh5; 1.
DR   SUPFAM; SSF109910; SSF109910; 1.
PE   3: Inferred from homology;
KW   Chaperone; Mitochondrion; Reference proteome.
FT   CHAIN           1..190
FT                   /note="Succinate dehydrogenase assembly factor 2,
FT                   mitochondrial"
FT                   /id="PRO_0000383197"
SQ   SEQUENCE   190 AA;  22414 MW;  3F25A2D97E63516B CRC64;
     METTCGLPVL PQKISVNFML RETVDDLGLS KSVTFFLLNS PIMLRLFQRT QFQGSKTCPR
     VFSKSFHSLP FLRQELILKV EPLKRDNESE DVKRRRLVYQ SRKRGILETD LLLSRFAKRY
     LPTMSVEEME EYDDLLNELD WDIYYWAVKN YEVTPLPEKW KDSKILAKLQ EMSANNEGEI
     LRMPNLDDSP