BFA1_YEAST
ID BFA1_YEAST Reviewed; 574 AA.
AC P47113; D6VWM4;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Mitotic check point protein BFA1;
DE AltName: Full=Cell cycle arrest protein BFA1;
GN Name=BFA1; OrderedLocusNames=YJR053W; ORFNames=J1667;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7668047; DOI=10.1002/yea.320110809;
RA Huang M.-E., Chuat J.-C., Galibert F.;
RT "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA
RT genes and 14 new open reading frames including a gene most probably
RT belonging to the family of ubiquitin-protein ligases.";
RL Yeast 11:775-781(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP CHARACTERIZATION.
RX PubMed=10220406; DOI=10.1073/pnas.96.9.4989;
RA Li R.;
RT "Bifurcation of the mitotic checkpoint pathway in budding yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:4989-4994(1999).
RN [5]
RP CHARACTERIZATION.
RX PubMed=11493673; DOI=10.1242/jcs.114.12.2345;
RA Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H.;
RT "The Bub2-dependent mitotic pathway in yeast acts every cell cycle and
RT regulates cytokinesis.";
RL J. Cell Sci. 114:2345-2354(2001).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Part of a checkpoint which monitors spindle integrity and
CC prevents premature exit from mitosis. This cell-cycle arrest depends
CC upon inhibition of the G-protein TEM1 by the BFA1/BUB2 complex.
CC -!- SUBUNIT: Interacts with BUB2.
CC -!- INTERACTION:
CC P47113; P26448: BUB2; NbExp=5; IntAct=EBI-3586, EBI-3824;
CC P47113; P38785: GIC1; NbExp=2; IntAct=EBI-3586, EBI-7575;
CC P47113; P38987: TEM1; NbExp=3; IntAct=EBI-3586, EBI-19113;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle pole.
CC -!- PTM: Multiply phosphorylated in a cell-cycle-dependent manner with the
CC major phosphorylation occurring in mitosis.
CC -!- MISCELLANEOUS: Present with 1380 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: To S.pombe byr4. {ECO:0000305}.
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DR EMBL; L36344; AAA88756.1; -; Genomic_DNA.
DR EMBL; Z49553; CAA89581.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08840.1; -; Genomic_DNA.
DR PIR; S57072; S57072.
DR RefSeq; NP_012587.3; NM_001181711.3.
DR AlphaFoldDB; P47113; -.
DR BioGRID; 33807; 379.
DR ComplexPortal; CPX-964; GTPase-Activating Protein BFA1-BUB2 complex.
DR DIP; DIP-4825N; -.
DR IntAct; P47113; 22.
DR MINT; P47113; -.
DR STRING; 4932.YJR053W; -.
DR iPTMnet; P47113; -.
DR MaxQB; P47113; -.
DR PaxDb; P47113; -.
DR PRIDE; P47113; -.
DR EnsemblFungi; YJR053W_mRNA; YJR053W; YJR053W.
DR GeneID; 853513; -.
DR KEGG; sce:YJR053W; -.
DR SGD; S000003814; BFA1.
DR VEuPathDB; FungiDB:YJR053W; -.
DR eggNOG; ENOG502R6H5; Eukaryota.
DR HOGENOM; CLU_037140_0_0_1; -.
DR InParanoid; P47113; -.
DR OMA; QEIDHNT; -.
DR BioCyc; YEAST:G3O-31687-MON; -.
DR PRO; PR:P47113; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47113; protein.
DR GO; GO:1990334; C:Bfa1-Bub2 complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0044732; C:mitotic spindle pole body; IBA:GO_Central.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0005816; C:spindle pole body; IDA:SGD.
DR GO; GO:0005092; F:GDP-dissociation inhibitor activity; IDA:SGD.
DR GO; GO:0005096; F:GTPase activator activity; IEA:InterPro.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0031578; P:mitotic spindle orientation checkpoint signaling; IDA:ComplexPortal.
DR GO; GO:0001100; P:negative regulation of exit from mitosis; IMP:SGD.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IDA:ComplexPortal.
DR GO; GO:0046578; P:regulation of Ras protein signal transduction; IBA:GO_Central.
DR InterPro; IPR034586; Bfa1/Byr4.
DR PANTHER; PTHR35140; PTHR35140; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Mitosis;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..574
FT /note="Mitotic check point protein BFA1"
FT /id="PRO_0000064913"
FT REGION 154..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..173
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 317
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 574 AA; 66086 MW; 1EA75D26FCF041DA CRC64;
MSIRPLTLNG LDEPETSFEE LNTTLPRFQS HETLTLEENV PPLSTSTYIP PPSSVGTSDT
GTVFSNSTSA FWSNKQADDD QDMEVDQDDE FLNDFQEFQN KKDDFDDAIK TNFHLRNGCR
TGPFKNDIFA EEFDRKLSLE DKPRLKQPRS MMELKPKRKL SNSVTSRNLR SGNSVRFKKS
MPNLALVNPA IREEEEDEER EREDQREFNY KIDNDTQDTI LAKFSSDDEG DFLTGFEELE
GEAIDETISS NDKESADHPR FLKKSSSSLP LKISPAQYDI VKHDELLTPG LHRRQRDWNT
QQELDSFKEK RSVRHCSNQN VQLNGPAKIK TIKQQIDHNT PMKKGSMIYN PKTMKWEGNE
NVLSKFSDVD TANRKALLIK NKLQRDADSK KQKYSDLQHA RATSRNQKVI GNMILDEQNL
RWVSVSEEEA DPFAGIPEIN LPPVGKSMKK RSSSPFLRSK SQVNTPFVSN DNDGVYQSTA
AQARLRKYHS MRTLNGTTET PEISSTFHLS SRALEKFYHE ENRWCKKLAS WFIPRDETII
SVDEETIMDE STVNSKRKSY MYEIRNMVIN STKD
