ID   BFD1_TOXGM              Reviewed;        2415 AA.
AC   S8GIB3;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   17-JUN-2020, sequence version 2.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Bradyzoite-formation deficient protein 1 {ECO:0000303|PubMed:31955846};
GN   Name=BFD1 {ECO:0000303|PubMed:31955846};
GN   ORFNames=TGME49_200385 {ECO:0000312|EMBL:EPT28189.1};
OS   Toxoplasma gondii (strain ATCC 50611 / Me49).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC   Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX   NCBI_TaxID=508771;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP   STAGE, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=31955846; DOI=10.1016/j.cell.2019.12.013;
RA   Waldman B.S., Schwarz D., Wadsworth M.H. II, Saeij J.P., Shalek A.K.,
RA   Lourido S.;
RT   "Identification of a master regulator of differentiation in Toxoplasma.";
RL   Cell 180:359-372(2020).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 50611 / Me49;
RA   Sibley D., Venepally P., Karamycheva S., Hadjithomas M., Khan A., Brunk B.,
RA   Roos D., Caler E., Lorenzi H.;
RL   Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Master transcription factor that controls the differentiation
CC       of acute-stage tachyzoite parasites into chronic-stage bradyzoites,
CC       which form intracellular cysts resistant to immune clearance and
CC       existing therapies (PubMed:31955846). Sufficient to drive
CC       differentiation into bradyzoite stage (PubMed:31955846). Following
CC       translation in response to stress conditions, binds to the promoter of
CC       many chronic stage-specific genes and promotes their expression,
CC       thereby driving differentiation into bradyzoites (PubMed:31955846).
CC       {ECO:0000269|PubMed:31955846}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31955846}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at a similar level during both the
CC       chronic and acute stages of infection (PubMed:31955846). However,
CC       transcripts are only translated during the chronic stage of infection,
CC       in response to stress conditions (PubMed:31955846).
CC       {ECO:0000269|PubMed:31955846}.
CC   -!- INDUCTION: Regulated at the translation level: while it is expressed
CC       during both the chronic and acute stages of infection, transcripts are
CC       only translated during the chronic stage of infection.
CC       {ECO:0000269|PubMed:31955846}.
CC   -!- DISRUPTION PHENOTYPE: Parasites grow normally under standard conditions
CC       but fail to differentiate into chronic-stage bradyzoites under all
CC       induction conditions tested (PubMed:31955846). Parasites are unable to
CC       form brain cysts in mice (PubMed:31955846).
CC       {ECO:0000269|PubMed:31955846}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EPT28189.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; KE138831; EPT28189.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_018636521.1; XM_018779152.1.
DR   AlphaFoldDB; S8GIB3; -.
DR   SMR; S8GIB3; -.
DR   EnsemblProtists; TGME49_200385-t26_1; TGME49_200385-t26_1; TGME49_200385.
DR   GeneID; 7895875; -.
DR   KEGG; tgo:TGME49_200385; -.
DR   Proteomes; UP000001529; Chromosome VIII.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IDA:UniProtKB.
DR   GO; GO:0045165; P:cell fate commitment; IDA:UniProtKB.
DR   GO; GO:0097436; P:entry into dormancy; IDA:UniProtKB.
DR   CDD; cd00167; SANT; 2.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017930; Myb_dom.
DR   InterPro; IPR001005; SANT/Myb.
DR   SMART; SM00717; SANT; 2.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS51294; HTH_MYB; 1.
DR   PROSITE; PS50090; MYB_LIKE; 1.
PE   2: Evidence at transcript level;
KW   Activator; Bradyzoite; Differentiation; DNA-binding; Nucleus;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..2415
FT                   /note="Bradyzoite-formation deficient protein 1"
FT                   /id="PRO_0000450214"
FT   DOMAIN          921..968
FT                   /note="Myb-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT   DOMAIN          969..1023
FT                   /note="HTH myb-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DNA_BIND        996..1019
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   REGION          369..392
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          418..481
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          761..845
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1027..1050
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1098..1127
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1206..1270
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1319..1343
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1501..1521
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1905..1932
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1959..2013
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          2161..2222
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        426..446
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        447..481
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        831..845
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1230..1259
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1329..1343
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1970..1988
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1989..2003
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        2166..2202
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   2415 AA;  262563 MW;  8D52E06589F19080 CRC64;
     MEGASTQPMP HSQRERQAPV HAISGTWENC DQAGEYKGTA CCVVERQVYS TETHAVEGCC
     STRWNGVKEE KGGGEVSSRT ALAGVVHLYT NSDGNAYTHI SDQGMRQDEA VAGQEQRHLN
     CEGEHEKKGN VAAGGGTLMV TKSEIEPCDD YYSVQRGQSC AGERAPRDGC CRLLDGSRVD
     PAEGGSEEDE NCYTHVLQKH LGQMPTAPYQ GDDRLQTDLR LGDFQSGPSD ALSGHHVTRT
     PILSPHGRYS EYISERLAWQ YAERPGHGIA AENTVVMHSH TGEATGSLRA DAPSQWSTES
     RLQFHVGSQF TTENPELFAG IVGLDTEQFD ARNAEALHWR QQGEARSTET ECIADITPER
     GNRSTIRQWN KPEAARQEYH RASASARGPE ETNIITRGAH SDDQSIDAAA PGCWAARHLT
     SRQHPNPRPR MKEEHCGRER EVLSSEQPSD CGETQKTPAS HSLVLDSKSR ADASQTSPYT
     RNDEIQTIRA FEEQCLEVQL PQSRLRRGID YEAGMSQLAP TTQELKAKVL TTGLSPSCPN
     AAVLRASVPL SMDTTVTVHL ADVEDPSSKR HTPIENSISS GHCDPPCSSP GGMMEPRVSV
     SGLGSPHNCV EDREIARHGD GIGRDILERR PLPFSPAALL TGCSSTAASS VVSGDVFTSV
     AASARANACR SSTDTQEESG CGDAPRYVLL FPDEQDERLG HAQTSIATPS GSGQSKCMFK
     GDGNAEATFR EEVKSSELRT PSRIQTRRLL PGVQLQGMDC DGCGASDPQK GRPAPETGFL
     PEICHFSPQH PWQPGSEVNQ GYRAGADYGT SRVQQSLENC SWEESVDEGE QPRTTSSSSY
     GQDTQKRDSF LQSIDNRIAS EPVDPTGTCG YNSTPETFRS GCIMRDQMLG VKPHLSHVSL
     TREGKHRGQQ THLRKTGVPT WSAEEDASLA ELVSRKGFKW ALISSQLTGA FGIPRTGKQC
     RERWFNHVNP EVKKGDWSAE EDAMILMLQN ELGNRWATIA KKLRGRTENA VKNRFISLSN
     ARLGYGRPKR DGSSADCFSN RRTGSGKSSG ITGMPNLCQS VCSAGTTKKD SSESGNHFVM
     SVATKVFEFS DVAVDSGVSR PRQCTGTSPS CGHPSAGEGD PSHLKNTDVV GREQPIQRNN
     NESGKAAEQT AFSGVKTGTL SVSQDAVPVG RLVVASVGPQ HIRRSFPTDE TLPKFAAKEH
     NNQQLNDERE HLEQSNSTSE GSFLASAHEH ADIARSDPDE DTLEPHQKRR RKRCAIAYQG
     EERGDSNGLD SIADRAEQAG NFQAMRKANT DNGKVDYLEP HRYEKLSPCE QVIQPSLRPA
     CDHRGAPQNS VESGEQSPDA QRQLCNQGCR TSNRTVHSSV YSNEVESNEL RGVFRLAEQS
     LPSQSGDPAW STAGFQLSIL PQKVEVHSRN KCDGQNVMYR CSPGSLPTTH QQTVFHYDRD
     SSRFPCAAKP AAASGAQGTI EENDGLVKEG PSMIVTGSSV EVVHCCSVSL RRRDRSLPSA
     QLWTSQETES DTNPSPNQQH ESCHQYCKRH AAWCGKTDQF SKLTSSHQEN SSGKDACLVS
     VSPTVTLDDL QKQSRGTVLS AKEEIGKPET WSHVVDNTYS KTDHQRASLC AENSSGCAEG
     STELVRFSGG SVKSGSSMSV DCGNGNPDDC QDCKAEEIWR GEQRYAERGH SVESRGAGSV
     GRSTDLTITD SGSMPLCASP IGRPPADNDT LFLSDARCNV VAQLNHQDNS RISRLASCEE
     EFLACGGERL INASGGFKPD GGCLYRMQQA GACNTKLHRP VHSCSTIDSE QLEDLPSVEK
     AVGDRSFSSK RKGDIPPFAE WKKNDELREL YRGVSEAVSH GQPGDWNGSW PGISGRAHQT
     SSCFPDRVNA SDRRELNSWR LHVSAAAELG SSHIWNSQSY ASASVSRDKQ REPPKNGLTG
     CDVPEYLGTS QSAGLPAANA HERGNFYGHD RCRPREGERV RWVGLQRNRK PEASVSSGAS
     NSATTARPKD STEPDEGNSE GVSTRRKDSG STAATISRAV SLGMVTPSAA CENSSSLTDT
     SPPLSHRPSF SFTHCCEETL SRCNSSNYLC PPATCHTSDD GRSLGPSREA QALRSLSLAS
     GYGYPGIPAE ATSFWQGSSL EHSIMEPQMV PSDDELRLWV HPRDAANWSQ STLKPVAVVS
     GTDAGDDQHK TPENLTPESG QAHRRDGHDM QRVQRCDDEG ECPPTTVELT FPHSHSSDEM
     QDLPSKVQGN FLLRRELSDS LQHETAESVA GYGWMRIRNA GDIPNSKVPC AWEQCMPASE
     RERGVNDHMS SEASRMSKAA SSSFVPSSCT DAPVVRVGED TTKSVCEEQQ LCEGGNRGSL
     SPEATGFESL GPPLQLLLVD GYTPFEPVVE KVSQTMEQTL FPVPGQETDT RDEDGRYNCE
     CLQNRQPPLH SGGLM