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SDIA_ECOLI
ID   SDIA_ECOLI              Reviewed;         240 AA.
AC   P07026; P76313;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-MAY-2022, entry version 156.
DE   RecName: Full=Regulatory protein SdiA;
GN   Name=sdiA; OrderedLocusNames=b1916, JW1901;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3515318; DOI=10.1093/nar/14.5.2301;
RA   Sharma S., Stark T.F., Beattie W.G., Moses R.E.;
RT   "Multiple control elements for the uvrC gene unit of Escherichia coli.";
RL   Nucleic Acids Res. 14:2301-2318(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION.
RX   PubMed=1915297; DOI=10.1002/j.1460-2075.1991.tb04900.x;
RA   Wang X., de Boer P.A.J., Rothfield L.I.;
RT   "A factor that positively regulates cell division by activating
RT   transcription of the major cluster of essential cell division genes of
RT   Escherichia coli.";
RL   EMBO J. 10:3363-3372(1991).
RN   [6]
RP   ROLE IN YDIV REGULATION, REPRESSION BY GLUCOSE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / W3110 / ZK126;
RX   PubMed=18560382; DOI=10.1038/cr.2008.67;
RA   Zhou X., Meng X., Sun B.;
RT   "An EAL domain protein and cyclic AMP contribute to the interaction between
RT   the two quorum sensing systems in Escherichia coli.";
RL   Cell Res. 18:937-948(2008).
RN   [7]
RP   STRUCTURE BY NMR OF 3-171 IN COMPLEX WITH AUTOINDUCER, AND BINDING OF
RP   HOMOSERINE LACTONE DERIVATIVES.
RX   PubMed=16307757; DOI=10.1016/j.jmb.2005.10.041;
RA   Yao Y., Martinez-Yamout M.A., Dickerson T.J., Brogan A.P., Wright P.E.,
RA   Dyson H.J.;
RT   "Structure of the Escherichia coli quorum sensing protein SdiA: activation
RT   of the folding switch by acyl homoserine lactones.";
RL   J. Mol. Biol. 355:262-273(2006).
CC   -!- FUNCTION: Activates cell division by specifically increasing
CC       transcription from one of the two promoters that lie immediately
CC       upstream of the ftsQAZ gene cluster. Activates ydiV expression in
CC       response to extracellular autoinducer AI-1 (Vibrio fischeri autoinducer
CC       oxoC6). {ECO:0000269|PubMed:18560382, ECO:0000269|PubMed:1915297}.
CC   -!- INDUCTION: Repressed by glucose. {ECO:0000269|PubMed:18560382}.
CC   -!- DOMAIN: Binding to the autoinducer occurs via the N-terminal 170
CC       residues; as E.coli does not produce LuxI autoinducers endogenously it
CC       should be able to bind to a number of different AI-1 autoinducers,
CC       which would enable it to detect other bacteria. This was shown to be
CC       the case.
CC   -!- DISRUPTION PHENOTYPE: A double sdiA/ydiV deletion mutant leads to
CC       decreased cAMP levels which inhibits quorum sensing system 2.
CC       {ECO:0000269|PubMed:18560382}.
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DR   EMBL; X03691; CAA27327.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74983.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15736.1; -; Genomic_DNA.
DR   PIR; A64955; QQECU1.
DR   RefSeq; NP_416426.1; NC_000913.3.
DR   RefSeq; WP_001152715.1; NZ_LN832404.1.
DR   PDB; 2AVX; NMR; -; A=1-171.
DR   PDB; 4LFU; X-ray; 2.26 A; A=1-240.
DR   PDB; 4LGW; X-ray; 2.70 A; A=1-240.
DR   PDBsum; 2AVX; -.
DR   PDBsum; 4LFU; -.
DR   PDBsum; 4LGW; -.
DR   AlphaFoldDB; P07026; -.
DR   BMRB; P07026; -.
DR   SMR; P07026; -.
DR   BioGRID; 4259602; 108.
DR   BioGRID; 850778; 3.
DR   IntAct; P07026; 8.
DR   STRING; 511145.b1916; -.
DR   ChEMBL; CHEMBL4523168; -.
DR   DrugBank; DB07928; N-(2-OXOTETRAHYDROFURAN-3-YL)OCTANAMIDE.
DR   PaxDb; P07026; -.
DR   PRIDE; P07026; -.
DR   EnsemblBacteria; AAC74983; AAC74983; b1916.
DR   EnsemblBacteria; BAA15736; BAA15736; BAA15736.
DR   GeneID; 946421; -.
DR   KEGG; ecj:JW1901; -.
DR   KEGG; eco:b1916; -.
DR   PATRIC; fig|1411691.4.peg.333; -.
DR   EchoBASE; EB0928; -.
DR   eggNOG; COG2197; Bacteria.
DR   HOGENOM; CLU_072786_7_1_6; -.
DR   InParanoid; P07026; -.
DR   OMA; NHTVDWY; -.
DR   PhylomeDB; P07026; -.
DR   BioCyc; EcoCyc:PD02198; -.
DR   EvolutionaryTrace; P07026; -.
DR   PRO; PR:P07026; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:2000144; P:positive regulation of DNA-templated transcription, initiation; IDA:EcoCyc.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.450.80; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR005143; TF_LuxR_autoind-bd_dom.
DR   InterPro; IPR036693; TF_LuxR_autoind-bd_dom_sf.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03472; Autoind_bind; 1.
DR   Pfam; PF00196; GerE; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF75516; SSF75516; 1.
DR   PROSITE; PS00622; HTH_LUXR_1; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Cell cycle; Cell division; DNA-binding;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..240
FT                   /note="Regulatory protein SdiA"
FT                   /id="PRO_0000184187"
FT   DOMAIN          173..238
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        197..216
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   CONFLICT        120..128
FT                   /note="TQYLMLPNR -> HSVFNAAQTG (in Ref. 1; CAA27327)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        140
FT                   /note="A -> R (in Ref. 1; CAA27327)"
FT                   /evidence="ECO:0000305"
FT   HELIX           6..19
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           23..36
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   STRAND          40..47
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   STRAND          51..54
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   STRAND          57..61
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           65..73
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           76..78
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           81..83
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           85..87
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   STRAND          92..94
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           97..100
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           104..112
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   STRAND          117..124
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   STRAND          130..138
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           147..167
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   TURN            171..173
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           182..192
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           197..204
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           208..222
FT                   /evidence="ECO:0007829|PDB:4LFU"
FT   HELIX           227..236
FT                   /evidence="ECO:0007829|PDB:4LFU"
SQ   SEQUENCE   240 AA;  28117 MW;  C04CCC50C06135C4 CRC64;
     MQDKDFFSWR RTMLLRFQRM ETAEEVYHEI ELQAQQLEYD YYSLCVRHPV PFTRPKVAFY
     TNYPEAWVSY YQAKNFLAID PVLNPENFSQ GHLMWNDDLF SEAQPLWEAA RAHGLRRGVT
     QYLMLPNRAL GFLSFSRCSA REIPILSDEL QLKMQLLVRE SLMALMRLND EIVMTPEMNF
     SKREKEILRW TAEGKTSAEI AMILSISENT VNFHQKNMQK KINAPNKTQV ACYAAATGLI
 
 
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