ID   SDIS_NOCSI              Reviewed;         123 AA.
AC   P77816; O08058;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Steroid Delta-isomerase;
DE            EC=5.3.3.1;
DE   AltName: Full=Delta(5)-3-ketosteroid isomerase;
GN   Name=ksdI;
OS   Nocardioides simplex (Arthrobacter simplex).
OC   Bacteria; Actinobacteria; Propionibacteriales; Nocardioidaceae;
OC   Pimelobacter.
OX   NCBI_TaxID=2045;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 6946 / DSM 20130 / BCRC 10381 / CCUG 23611 / CIP 82.106 / JCM
RC   1363 / NBRC 12069 / NCIMB 8929 / NCTC 4215 / NRRL B-14051;
RX   PubMed=7596291; DOI=10.1111/j.1365-2958.1995.tb02359.x;
RA   Molnar I., Choi K.P., Yamashita M., Murooka Y.;
RT   "Molecular cloning, expression in Streptomyces lividans, and analysis of a
RT   gene cluster from Arthrobacter simplex encoding 3-ketosteroid-delta 1-
RT   dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical
RT   regulatory protein.";
RL   Mol. Microbiol. 15:895-905(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 6946 / DSM 20130 / BCRC 10381 / CCUG 23611 / CIP 82.106 / JCM
RC   1363 / NBRC 12069 / NCIMB 8929 / NCTC 4215 / NRRL B-14051;
RA   Dziadek J., Yamashita M., Murooka Y.;
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid;
CC         Xref=Rhea:RHEA:14709, ChEBI:CHEBI:47907, ChEBI:CHEBI:47909;
CC         EC=5.3.3.1;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
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DR   EMBL; D37969; BAA07187.1; -; Genomic_DNA.
DR   EMBL; Z93338; CAB07540.1; -; Genomic_DNA.
DR   PIR; S61890; S61890.
DR   RefSeq; WP_038682822.1; NZ_FOUK01000004.1.
DR   AlphaFoldDB; P77816; -.
DR   SMR; P77816; -.
DR   STRING; 2045.KR76_27120; -.
DR   eggNOG; COG3631; Bacteria.
DR   OMA; VEPIDVM; -.
DR   OrthoDB; 1644255at2; -.
DR   GO; GO:0004769; F:steroid delta-isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR037401; SnoaL-like.
DR   Pfam; PF12680; SnoaL_2; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   3: Inferred from homology;
KW   Isomerase; Lipid metabolism; Steroid metabolism.
FT   CHAIN           1..123
FT                   /note="Steroid Delta-isomerase"
FT                   /id="PRO_0000097645"
FT   ACT_SITE        12
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        36
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         96
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   123 AA;  12677 MW;  F08AF72E2F3858B6 CRC64;
     MSAEVKAAVA RYLDAVAGGS PAAIAALYAP DATLEDPVGA DLVRGRAAIE EFYGALAGAK
     VSTELLAVRA VAGHAAFSFR VTTDAGDQQY VVEPIDVMTF DADGQITSMR AFWAPGDMVV
     TPA