ID   SDMT_APHHA              Reviewed;         277 AA.
AC   Q83WC3;
DT   21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Sarcosine/dimethylglycine N-methyltransferase;
DE            EC=2.1.1.157;
DE   AltName: Full=Dimethylglycine N-methyltransferase;
OS   Aphanothece halophytica.
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Aphanothecaceae; Aphanothece.
OX   NCBI_TaxID=72020;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A METHYLTRANSFERASE,
RP   FUNCTION IN BETAINE BIOSYNTHESIS, CATALYTIC ACTIVITY, MUTAGENESIS OF
RP   PRO-171 AND MET-172, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, SUBSTRATE
RP   SPECIFICITY, ACTIVITY REGULATION, SUBUNIT, AND NOMENCLATURE.
RX   PubMed=12466265; DOI=10.1074/jbc.m210970200;
RA   Waditee R., Tanaka Y., Aoki K., Hibino T., Jikuya H., Takano J., Takabe T.,
RA   Takabe T.;
RT   "Isolation and functional characterization of N-methyltransferases that
RT   catalyze betaine synthesis from glycine in a halotolerant photosynthetic
RT   organism Aphanothece halophytica.";
RL   J. Biol. Chem. 278:4932-4942(2003).
CC   -!- FUNCTION: Catalyzes the methylation of sarcosine and dimethylglycine to
CC       dimethylglycine and betaine, respectively, with S-adenosylmethionine
CC       (AdoMet) acting as the methyl donor. {ECO:0000269|PubMed:12466265}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 S-adenosyl-L-methionine + sarcosine = glycine betaine + 2
CC         H(+) + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:32467,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17750, ChEBI:CHEBI:57433,
CC         ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.157;
CC         Evidence={ECO:0000269|PubMed:12466265};
CC   -!- ACTIVITY REGULATION: Inhibited by n-butylic acid and S-adenosyl-L-
CC       homocysteine. {ECO:0000269|PubMed:12466265}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.18 mM for S-adenosyl-L-homocysteine (at pH 8.8 and at 37 degrees
CC         Celsius) {ECO:0000269|PubMed:12466265};
CC         KM=0.5 mM for dimethylglycine (at pH 8.8 and at 37 degrees Celsius)
CC         {ECO:0000269|PubMed:12466265};
CC         Vmax=0.47 umol/min/mg enzyme (at pH 8.8 and at 37 degrees Celsius)
CC         {ECO:0000269|PubMed:12466265};
CC       pH dependence:
CC         Optimum pH is 8.8. The activities remain high at more alkaline pH but
CC         decrease sharply at the acidic side of the optimal pH.
CC         {ECO:0000269|PubMed:12466265};
CC   -!- PATHWAY: Amine and polyamine biosynthesis; betaine biosynthesis via
CC       glycine pathway; betaine from glycine: step 2/3.
CC       {ECO:0000269|PubMed:12466265}.
CC   -!- PATHWAY: Amine and polyamine biosynthesis; betaine biosynthesis via
CC       glycine pathway; betaine from glycine: step 3/3.
CC       {ECO:0000269|PubMed:12466265}.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:12466265}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB094498; BAC56940.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q83WC3; -.
DR   SMR; Q83WC3; -.
DR   KEGG; ag:BAC56940; -.
DR   BRENDA; 2.1.1.157; 383.
DR   UniPathway; UPA00530; UER00382.
DR   UniPathway; UPA00530; UER00383.
DR   GO; GO:0052729; F:dimethylglycine N-methyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0052730; F:sarcosine N-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019286; P:glycine betaine biosynthetic process from glycine; IDA:UniProtKB.
DR   GO; GO:0032259; P:methylation; IDA:UniProtKB.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   1: Evidence at protein level;
KW   Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..277
FT                   /note="Sarcosine/dimethylglycine N-methyltransferase"
FT                   /id="PRO_0000412535"
FT   SITE            171
FT                   /note="Important for dimethylglycine binding"
FT                   /evidence="ECO:0000305"
FT   SITE            172
FT                   /note="Important for dimethylglycine binding"
FT                   /evidence="ECO:0000305"
FT   MUTAGEN         171
FT                   /note="P->Q: The Vmax value is about 27% of the wild-type
FT                   when dimethylglycine is used as a methyl group acceptor.
FT                   Glycine and sarcosine are ineffective as methyl group
FT                   acceptors. The affinity for dimethylglycine decreases about
FT                   15-fold, and 2-fold for AdoMet. Almost inactive; when
FT                   associated with R-172."
FT                   /evidence="ECO:0000269|PubMed:12466265"
FT   MUTAGEN         172
FT                   /note="M->R: Almost inactive; when associated with Q-171."
FT                   /evidence="ECO:0000269|PubMed:12466265"
SQ   SEQUENCE   277 AA;  31613 MW;  77DE28246A030775 CRC64;
     MTKADAVAKQ AQDYYDSGSA DGFYYRIWGG EDLHIGIYNT PDEPIYDASV RTVSRICDKI
     KNWPAGTKVL DLGAGYGGSA RYMAKHHGFD VDCLNISLVQ NERNRQMNQE QGLADKIRVF
     DGSFEELPFE NKSYDVLWSQ DSILHSGNRR KVMEEADRVL KSGGDFVFTD PMQTDNCPEG
     VLEPVLARIH LDSLGSVGFY RQVAEELGWE FVEFDEQTHQ LVNHYSRVLQ ELEAHYDQLQ
     PECSQEYLDR MKVGLNHWIN AGKSGYMAWG ILKFHKP