位置:首页 > 蛋白库 > SDNR_SORAA
SDNR_SORAA
ID   SDNR_SORAA              Reviewed;         547 AA.
AC   A0A1B4XBI2;
DT   30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2016, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Beta-lactamase-like protein sdnR {ECO:0000303|PubMed:27072286};
DE            EC=3.5.-.- {ECO:0000305|PubMed:27072286};
DE   AltName: Full=Sordarin/hypoxysordarin biosynthesis cluster protein R {ECO:0000303|PubMed:27072286};
DE   Flags: Precursor;
GN   Name=sdnR {ECO:0000303|PubMed:27072286};
OS   Sordaria araneosa (Pleurage araneosa).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Sordaria.
OX   NCBI_TaxID=573841;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC   STRAIN=ATCC 36386 / NRRL 3196;
RX   PubMed=27072286; DOI=10.1038/ja.2016.40;
RA   Kudo F., Matsuura Y., Hayashi T., Fukushima M., Eguchi T.;
RT   "Genome mining of the sordarin biosynthetic gene cluster from Sordaria
RT   araneosa Cain ATCC 36386: characterization of cycloaraneosene synthase and
RT   GDP-6-deoxyaltrose transferase.";
RL   J. Antibiot. 69:541-548(2016).
CC   -!- FUNCTION: Beta-lactamase-like protein; part of the gene cluster that
CC       mediates the biosynthesis of sordarin and hypoxysordarin, glycoside
CC       antibiotics with a unique tetracyclic diterpene aglycone structure
CC       (PubMed:27072286). First, the geranylgeranyl diphosphate synthase sdnC
CC       constructs GGDP from farnesyl diphosphate and isopentenyl diphosphate
CC       (PubMed:27072286). The diterpene cyclase sdnA then catalyzes the
CC       cyclization of GGDP to afford cycloaraneosene (PubMed:27072286).
CC       Cycloaraneosene is then hydroxylated four times by the putative
CC       cytochrome P450 monooxygenases sdnB, sdnE, sdnF and sdnH to give a
CC       hydroxylated cycloaraneosene derivative such as cycloaraneosene-
CC       8,9,13,19-tetraol (PubMed:27072286). Although the order of the
CC       hydroxylations is unclear, at least C8, C9 and C13 of the
CC       cycloaraneosene skeleton are hydroxylated before the sordaricin
CC       formation (PubMed:27072286). Dehydration of the 13-hydroxy group of the
CC       hydroxylated cycloaraneosene derivative might be catalyzed by an
CC       unassigned hypothetical protein such as sdnG and sdnP to construct the
CC       cyclopentadiene moiety (PubMed:27072286). The FAD-dependent
CC       oxidoreductase sdnN is proposed to catalyze the oxidation at C9 of the
CC       hydroxylated cycloaraneosene derivative and also catalyze the Baeyer-
CC       Villiger oxidation to give the lactone intermediate (PubMed:27072286).
CC       The presumed lactone intermediate would be hydrolyzed to give an
CC       acrolein moiety and a carboxylate moiety (PubMed:27072286). Then,
CC       [4+2]cycloaddition would occur between the acrolein moiety and the
CC       cyclopentadiene moiety to give sordaricin (PubMed:27072286). SdnN might
CC       also be involved in the [4+2]cycloaddition after the hypothesized
CC       oxidation to accommodate the oxidized product and prompt the
CC       [4+2]cycloaddition (PubMed:27072286). GDP-6-deoxy-D-altrose may be
CC       biosynthesized from GDP-D-mannose by the putative GDP-mannose-4,6-
CC       dehydratase sdnI and the short-chain dehydrogenase sdnK
CC       (PubMed:27072286). The glycosyltransferase sdnJ catalyzes the
CC       attachment of 6-deoxy-D-altrose onto the 19-hydroxy group of sordaricin
CC       to give 4'-O-demethylsordarin (PubMed:27072286). The methyltransferase
CC       sdnD would complete the biosynthesis of sordarin (PubMed:27072286).
CC       Sordarin can be further modified into hypoxysordarin (PubMed:27072286).
CC       The unique acyl chain at the 3'-hydroxy group of hypoxysordarin would
CC       be constructed by an iterative type I PKS sdnO and the trans-acting
CC       polyketide methyltransferase sdnL. SdnL would be responsible for the
CC       introduction of an alpha-methyl group of the polyketide chain
CC       (PubMed:27072286). Alternatively, the beta-lactamase-like protein sdnR
CC       might be responsible for the cleavage and transfer of the polyketide
CC       chain from the PKS sdnO to sordarin (PubMed:27072286). Two putative
CC       cytochrome P450 monooxygenases, sdnQ and sdnT, might catalyze the
CC       epoxidations of the polyketide chain to complete the biosynthesis of
CC       hypoxysordarin (PubMed:27072286). Transcriptional regulators sdnM and
CC       sdnS are presumably encoded for the transcriptional regulation of the
CC       expression of the sdn gene cluster (PubMed:27072286).
CC       {ECO:0000269|PubMed:27072286}.
CC   -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000305|PubMed:27072286}.
CC   -!- SIMILARITY: Belongs to the class-C beta-lactamase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; LC079035; BAV32162.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1B4XBI2; -.
DR   SMR; A0A1B4XBI2; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR001466; Beta-lactam-related.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   Pfam; PF00144; Beta-lactamase; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Glycoprotein; Hydrolase; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..547
FT                   /note="Beta-lactamase-like protein sdnR"
FT                   /id="PRO_5008572238"
FT   ACT_SITE        102
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P05193"
FT   ACT_SITE        212
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P05193"
FT   CARBOHYD        57
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        211
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        226
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        245
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        256
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        341
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        424
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        446
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   547 AA;  59275 MW;  A501840B74350CEE CRC64;
     MKSFSPSTLV WIAVASQLGT AQNCPILGPA YPAVANLAAP KLTAAKARFE KALESINRTS
     TSFAVQVYSA HDDGLIYSTY NTATERVGAA TVGPDTIWRL FSISKAITVY AFLAKLGDGY
     WNEPITKYIA ELADAPVRDP IRDVNWAEVT VGSLAGQSSG LTRDYSLRDR STVQTQIPGF
     RELKDSEIVQ CEALRMIMKT YPWAPSYRTP NYSTMAFQLL AYAAENITGE SFPDIVVNEL
     FKPLNLTRSY VPIPRNETNI VNYPKEAWEL DLGDLSPGGG YSASASDLSA LGRSILRSTL
     LPPILTRRWL APTIHTGTVW HSMGRPWEIM RHRVPATPAS NVTRNVDIYA KQGGGGVYTT
     AIALSPDHDI GISVMTAGDE TQAAFNTIRE AFLDIWLGAA EEAARDLAEA TYAGSYAAKT
     AGDNSSIVVG LSPGEPALFV SELISNGTDV LAFAVNDAGR GGKGGQFGLW LYPMGLVDER
     EKGKKRVAFR GWPGVVGVEL EEACGSWAES DRLRWGNYPG DSYVFVVEGG KASTVENPGL
     GRTFWRV
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024