ID   SDPB_BACSU              Reviewed;         323 AA.
AC   O34616;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Sporulation-delaying protein SdpB;
GN   Name=sdpB {ECO:0000303|PubMed:12817086}; Synonyms=yvaX;
GN   OrderedLocusNames=BSU33760;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RA   Nakamura A., Grau R., Perego M., Hoch J.A.;
RL   Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   REQUIRED FOR SDPC ACTIVITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / PY79;
RX   PubMed=12817086; DOI=10.1126/science.1086462;
RA   Gonzalez-Pastor J.E., Hobbs E.C., Losick R.;
RT   "Cannibalism by sporulating bacteria.";
RL   Science 301:510-513(2003).
RN   [4]
RP   REPRESSION BY ABRB AND ABH.
RX   PubMed=17720793; DOI=10.1128/jb.01081-07;
RA   Strauch M.A., Bobay B.G., Cavanagh J., Yao F., Wilson A., Le Breton Y.;
RT   "Abh and AbrB control of Bacillus subtilis antimicrobial gene expression.";
RL   J. Bacteriol. 189:7720-7732(2007).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / PY79;
RX   PubMed=23687264; DOI=10.1128/jb.00407-13;
RA   Perez Morales T.G., Ho T.D., Liu W.T., Dorrestein P.C., Ellermeier C.D.;
RT   "Production of the cannibalism toxin SDP is a multistep process that
RT   requires SdpA and SdpB.";
RL   J. Bacteriol. 195:3244-3251(2013).
CC   -!- FUNCTION: Required for the maturation of SdpC to SDP (PubMed:12817086).
CC       Not required for SdpC signal peptide cleavage, secretion from the cell
CC       or disulfide bond formation (PubMed:23687264).
CC       {ECO:0000269|PubMed:12817086, ECO:0000269|PubMed:23687264}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: By Spo0A during nutrient starvation (PubMed:12817086).
CC       Repressed by AbrB during regular growth when nutrients are plentiful,
CC       in association with the transcriptional repressor Abh.
CC       {ECO:0000269|PubMed:12817086, ECO:0000269|PubMed:17720793}.
CC   -!- DISRUPTION PHENOTYPE: When the sdpA-sdpB-sdpC operon is deleted,
CC       increased rate of spore formation; a double operon deletion (sdpA-sdpC
CC       plus skfA-skfH) makes spores even faster (PubMed:12817086). A single
CC       deletion mutant does not have SDP activity (active peptide of sdpC)
CC       (PubMed:23687264). {ECO:0000269|PubMed:12817086,
CC       ECO:0000269|PubMed:23687264}.
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DR   EMBL; AB006738; BAA21901.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15381.1; -; Genomic_DNA.
DR   PIR; A70029; A70029.
DR   RefSeq; NP_391256.1; NC_000964.3.
DR   RefSeq; WP_003228363.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O34616; -.
DR   STRING; 224308.BSU33760; -.
DR   TCDB; 9.A.31.1.1; the putative sdpab peptide antibiotic-like killing factor exporter (sdpab) family.
DR   PaxDb; O34616; -.
DR   DNASU; 936240; -.
DR   EnsemblBacteria; CAB15381; CAB15381; BSU_33760.
DR   GeneID; 936240; -.
DR   KEGG; bsu:BSU33760; -.
DR   PATRIC; fig|224308.179.peg.3661; -.
DR   eggNOG; ENOG502ZGQB; Bacteria.
DR   InParanoid; O34616; -.
DR   BioCyc; BSUB:BSU33760-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008488; F:gamma-glutamyl carboxylase activity; IEA:InterPro.
DR   GO; GO:0030152; P:bacteriocin biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0017187; P:peptidyl-glutamic acid carboxylation; IEA:InterPro.
DR   InterPro; IPR011020; HTTM.
DR   InterPro; IPR023894; Sporulation_SdpB.
DR   InterPro; IPR007782; VKG_COase.
DR   Pfam; PF05090; VKG_Carbox; 1.
DR   SMART; SM00752; HTTM; 1.
DR   TIGRFAMs; TIGR04033; export_SdpB; 1.
PE   2: Evidence at transcript level;
KW   Antibiotic biosynthesis; Bacteriocin biosynthesis; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..323
FT                   /note="Sporulation-delaying protein SdpB"
FT                   /id="PRO_0000049937"
FT   TRANSMEM        27..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        112..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        155..177
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        219..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        248..270
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   323 AA;  37450 MW;  16187600AA00AD74 CRC64;
     MKILNSLEGY IDTYNPWKNT YALFRSLLGF STLLVLLFNS TDILFSYSAN NVTCENVYIP
     TAFCFAKEYS INFEIIRYLM IFILTLVVIG WRPRFTGLFH WYICYSIQTS ALTIDGGEQI
     ATVLSFLILP VTLLDSRRNH WNIKKNNNES FTKKTVLFYI MTIIKIQVFI IYLNAALERL
     KNKEWAEGTA IYYFFSDPVF GLPEYQLNLM NPLLESNFIV VITWLVTIFE LFLAASIISN
     IRIKRIALVL GILFHIGIIF SIGIVSFGLI MISALIIYLH PVQQNITMNW CSPLFKYIYV
     KGKRNFKRIG GESVKFLTKL FHS