SDPI_BACSU
ID SDPI_BACSU Reviewed; 207 AA.
AC O32241;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Immunity protein SdpI;
GN Name=sdpI; Synonyms=yvaZ; OrderedLocusNames=BSU33780;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP FUNCTION, INDUCTION, AND MUTAGENESIS OF ILE-6; ILE-50; PHE-78; MET-84;
RP LEU-85; ILE-98; GLN-126 AND SER-156.
RC STRAIN=168 / PY79;
RX PubMed=16469701; DOI=10.1016/j.cell.2005.11.041;
RA Ellermeier C.D., Hobbs E.C., Gonzalez-Pastor J.E., Losick R.;
RT "A three-protein signaling pathway governing immunity to a bacterial
RT cannibalism toxin.";
RL Cell 124:549-559(2006).
RN [3]
RP DISRUPTION PHENOTYPE.
RX PubMed=16629676; DOI=10.1111/j.1365-2958.2006.05131.x;
RA Butcher B.G., Helmann J.D.;
RT "Identification of Bacillus subtilis sigma-dependent genes that provide
RT intrinsic resistance to antimicrobial compounds produced by Bacilli.";
RL Mol. Microbiol. 60:765-782(2006).
RN [4]
RP FUNCTION.
RC STRAIN=168 / PY79;
RX PubMed=22469514; DOI=10.1111/j.1365-2958.2012.08038.x;
RA Lamsa A., Liu W.T., Dorrestein P.C., Pogliano K.;
RT "The Bacillus subtilis cannibalism toxin SDP collapses the proton motive
RT force and induces autolysis.";
RL Mol. Microbiol. 84:486-500(2012).
RN [5]
RP INDUCTION.
RC STRAIN=168 / PY79;
RX PubMed=23687264; DOI=10.1128/jb.00407-13;
RA Perez Morales T.G., Ho T.D., Liu W.T., Dorrestein P.C., Ellermeier C.D.;
RT "Production of the cannibalism toxin SDP is a multistep process that
RT requires SdpA and SdpB.";
RL J. Bacteriol. 195:3244-3251(2013).
CC -!- FUNCTION: Immunity protein that provides protection for the cell
CC against the toxic effects of SDP, its own SdpC-derived killing factor,
CC and that functions as a receptor/signal transduction protein as well.
CC Once SDP accumulates in the extracellular milieu, SdpI binds to SDP,
CC causing sequestration of SdpR at the bacterial membrane.
CC {ECO:0000269|PubMed:16469701, ECO:0000269|PubMed:22469514}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: By Spo0A during nutrient starvation, through its direct
CC negative control of AbrB, repressed by AbrB during regular growth when
CC nutrients are plentiful, and also by SdpR (PubMed:16469701). Induced by
CC expression of SDP (active peptide of sdpC) (PubMed:16469701,
CC PubMed:23687264). {ECO:0000269|PubMed:16469701,
CC ECO:0000269|PubMed:23687264}.
CC -!- DISRUPTION PHENOTYPE: Results in increased SigW expression which is
CC dependent on SdpC. Results in weaker growth (70% of wild-type cell
CC density) on LB medium, with more pronounced effects on sporulation
CC medium (15% of wild-type). Concomitant deletion of SigW results in
CC greatly diminished growth (5% of wild-type on LB and 3% on sporulation
CC medium). Over time, single SdpI mutant stops growing on sporulation
CC medium, while the SdpI SigW double mutant eventually lyses. Deletion of
CC RsiW restores growth of the SdpI single mutant to near wild-type
CC levels. {ECO:0000269|PubMed:16629676}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=I'll have you for supper
CC - Issue 90 of January 2008;
CC URL="https://web.expasy.org/spotlight/back_issues/090";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL009126; CAB15383.1; -; Genomic_DNA.
DR PIR; C70029; C70029.
DR RefSeq; NP_391258.1; NC_000964.3.
DR RefSeq; WP_003228357.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; O32241; -.
DR STRING; 224308.BSU33780; -.
DR TCDB; 9.A.32.1.1; the sdpc (peptide-antibiotic killer factor) immunity protein, sdpi (sdpi) family.
DR PaxDb; O32241; -.
DR EnsemblBacteria; CAB15383; CAB15383; BSU_33780.
DR GeneID; 936238; -.
DR KEGG; bsu:BSU33780; -.
DR PATRIC; fig|224308.179.peg.3663; -.
DR eggNOG; COG5658; Bacteria.
DR InParanoid; O32241; -.
DR OMA; LCIYAGA; -.
DR BioCyc; BSUB:BSU33780-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009636; P:response to toxic substance; IDA:UniProtKB.
DR InterPro; IPR012867; DUF1648.
DR InterPro; IPR026272; SdpI.
DR InterPro; IPR025962; SdpI/YhfL.
DR PANTHER; PTHR37810:SF5; PTHR37810:SF5; 1.
DR Pfam; PF07853; DUF1648; 1.
DR Pfam; PF13630; SdpI; 1.
DR PIRSF; PIRSF038959; SdpI; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..207
FT /note="Immunity protein SdpI"
FT /id="PRO_0000312744"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 6
FT /note="I->T: Ability to bypass the requirement of SdpC
FT binding for expression of the sdpRI genes."
FT /evidence="ECO:0000269|PubMed:16469701"
FT MUTAGEN 50
FT /note="I->T: Ability to bypass the requirement of SdpC
FT binding for expression of the sdpRI genes."
FT /evidence="ECO:0000269|PubMed:16469701"
FT MUTAGEN 78
FT /note="F->I: Ability to bypass the requirement of SdpC
FT binding for expression of the sdpRI genes."
FT /evidence="ECO:0000269|PubMed:16469701"
FT MUTAGEN 84
FT /note="M->T: Ability to bypass the requirement of SdpC
FT binding for expression of the sdpRI genes."
FT /evidence="ECO:0000269|PubMed:16469701"
FT MUTAGEN 85
FT /note="L->S: Ability to bypass the requirement of SdpC
FT binding for expression of the sdpRI genes."
FT /evidence="ECO:0000269|PubMed:16469701"
FT MUTAGEN 98
FT /note="I->K: Ability to bypass the requirement of SdpC
FT binding for expression of the sdpRI genes."
FT /evidence="ECO:0000269|PubMed:16469701"
FT MUTAGEN 126
FT /note="Q->L: Loss of ability to promote transcription of
FT the sdpRI genes in response to SdpC binding. No effect in
FT ability to confer immunity to SdpC."
FT /evidence="ECO:0000269|PubMed:16469701"
FT MUTAGEN 156
FT /note="S->T: Loss of ability to promote transcription of
FT the sdpRI genes in response to SdpC binding. No effect in
FT ability to confer immunity to SdpC."
FT /evidence="ECO:0000269|PubMed:16469701"
SQ SEQUENCE 207 AA; 23293 MW; 27171B056BA86490 CRC64;
MKKNIISIII VCLSFLTSII LYQYLPEEIP IQWSGNKPAA IVSKPLTIFI IPVVMLIYYL
TFYMLTIKST QKNKALLFLA SNNMLILLYI LQLSTLLISL GYEVNIDLII GLGVGIFLII
GGNSMQLAEQ NHLIGLRTPW TLKDETVWKL GNRFASKVLV VCGFIIAVLS FFTGEYIILI
MIVLVLLALV ISTLASYHYY KKLNGSR
