SDR1_PAPSO
ID SDR1_PAPSO Reviewed; 348 AA.
AC I3PLR3;
DT 03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2012, sequence version 1.
DT 03-AUG-2022, entry version 28.
DE RecName: Full=Noscapine synthase SDR1 {ECO:0000305};
DE EC=1.1.1.415 {ECO:0000269|PubMed:27378283};
DE AltName: Full=Short-chain dehydrogenase/reductase 1 {ECO:0000303|PubMed:22653730};
DE Short=PsSDR1 {ECO:0000303|PubMed:22653730};
GN Name=SDR1 {ECO:0000303|PubMed:22653730};
OS Papaver somniferum (Opium poppy).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ranunculales; Papaveraceae; Papaveroideae;
OC Papaver.
OX NCBI_TaxID=3469;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=22653730; DOI=10.1126/science.1220757;
RA Winzer T., Gazda V., He Z., Kaminski F., Kern M., Larson T.R., Li Y.,
RA Meade F., Teodor R., Vaistij F.E., Walker C., Bowser T.A., Graham I.A.;
RT "A Papaver somniferum 10-gene cluster for synthesis of the anticancer
RT alkaloid noscapine.";
RL Science 336:1704-1708(2012).
RN [2]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=27378283; DOI=10.1038/ncomms12137;
RA Li Y., Smolke C.D.;
RT "Engineering biosynthesis of the anticancer alkaloid noscapine in yeast.";
RL Nat. Commun. 7:12137-12137(2016).
RN [3]
RP FUNCTION.
RX PubMed=29610307; DOI=10.1073/pnas.1721469115;
RA Li Y., Li S., Thodey K., Trenchard I., Cravens A., Smolke C.D.;
RT "Complete biosynthesis of noscapine and halogenated alkaloids in yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:E3922-E3931(2018).
CC -!- FUNCTION: Oxidoreductase that catalyzes the last step in the
CC biosynthesis of the benzylisoquinoline alkaloid noscapine
CC (PubMed:27378283, PubMed:29610307). Converts narcotine hemiacetal to
CC noscapine (PubMed:27378283). {ECO:0000269|PubMed:27378283,
CC ECO:0000269|PubMed:29610307}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + narcotine hemiacetal = H(+) + NADH + noscapine;
CC Xref=Rhea:RHEA:57404, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:73237, ChEBI:CHEBI:141667;
CC EC=1.1.1.415; Evidence={ECO:0000269|PubMed:27378283};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57405;
CC Evidence={ECO:0000305|PubMed:27378283};
CC -!- PATHWAY: Alkaloid biosynthesis. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. {ECO:0000305}.
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DR EMBL; JQ659007; AFB74619.1; -; Genomic_DNA.
DR AlphaFoldDB; I3PLR3; -.
DR SMR; I3PLR3; -.
DR EnsemblPlants; RZC84727; RZC84727; C5167_047502.
DR Gramene; RZC84727; RZC84727; C5167_047502.
DR KEGG; ag:AFB74619; -.
DR BioCyc; MetaCyc:MON-17772; -.
DR BRENDA; 1.1.1.415; 4515.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR001509; Epimerase_deHydtase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01370; Epimerase; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Alkaloid metabolism; NAD; Oxidoreductase.
FT CHAIN 1..348
FT /note="Noscapine synthase SDR1"
FT /id="PRO_0000447601"
SQ SEQUENCE 348 AA; 38815 MW; CD352C38CF59545F CRC64;
MHGQKNISER YQKFKEMEGT GKIVCVTGGA GYLASWLIMR LLERGYSVRT TVRSDPKFRE
DVSHLKALPE ATEKLQIFEA DLENPESFDD AINGCVGVFL VAQGMNFAEE YTLEKIIKTC
VEGTLRILQS CLKSKTVKKV VYTSSADAAM MISNLKAVKE IDETIWSEVD NFISKPEQVI
PGLPSYVVSK VLTERACLKF SEEHGLDVVT ILPPLVVGPF ITPHPPPSVS IALSIISGDV
SMMLGVRLEN AVHIDDVALA HIFVFECEKA KGRHICSSVD FPMHDLPKFI SENYPEFNVP
TDLLKDIEEQ EPVHLSSDKL LSMGFQFKYD FAEIFGDAIR CAKEKGFL
