SDR2B_ARATH
ID SDR2B_ARATH Reviewed; 296 AA.
AC Q9ZUH5; A8MRH1;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Short-chain dehydrogenase/reductase 2b;
DE Short=AtSDR2b;
DE EC=1.1.1.-;
GN Name=SDR2b; Synonyms=SDR2; OrderedLocusNames=At2g24190; ORFNames=F27D4.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP INDUCTION.
RX PubMed=18599651; DOI=10.1104/pp.108.119461;
RA Choi H.W., Lee B.G., Kim N.H., Park Y., Lim C.W., Song H.K., Hwang B.K.;
RT "A role for a menthone reductase in resistance against microbial pathogens
RT in plants.";
RL Plant Physiol. 148:383-401(2008).
RN [4]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=21169366; DOI=10.1074/jbc.m110.202226;
RA Yamauchi Y., Hasegawa A., Taninaka A., Mizutani M., Sugimoto Y.;
RT "NADPH-dependent reductases involved in the detoxification of reactive
RT carbonyls in plants.";
RL J. Biol. Chem. 286:6999-7009(2011).
CC -!- FUNCTION: Aldehyde reductase that catalyzes the reduction of the
CC aldehyde carbonyl groups on saturated and alpha,beta-unsaturated
CC aldehydes with more than 5 carbons (PubMed:21169366).
CC {ECO:0000269|PubMed:21169366}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=40 mM for butyraldehyde {ECO:0000269|PubMed:21169366};
CC KM=0.2 mM for (E)-2-pentenal {ECO:0000269|PubMed:21169366};
CC KM=1.7 mM for (E)-2-hexenal {ECO:0000269|PubMed:21169366};
CC KM=69 mM for methylglyoxal {ECO:0000269|PubMed:21169366};
CC Note=kcat is 9.1 sec(-1) for butyraldehyde. kcat is 0.4 sec(-1) for
CC (E)-2-pentenal. kcat is 0.6 sec(-1) for (E)-2-hexenal. kcat is 24
CC sec(-1) for methylglyoxal. {ECO:0000269|PubMed:21169366};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ZUH5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZUH5-2; Sequence=VSP_035197;
CC -!- INDUCTION: Not induced by pathogen infection and not detected in
CC healthy leaves. {ECO:0000269|PubMed:18599651}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AC005967; AAD03380.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07537.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07538.1; -; Genomic_DNA.
DR PIR; F84633; F84633.
DR RefSeq; NP_001077951.1; NM_001084482.3. [Q9ZUH5-2]
DR RefSeq; NP_179996.1; NM_127980.4. [Q9ZUH5-1]
DR AlphaFoldDB; Q9ZUH5; -.
DR SMR; Q9ZUH5; -.
DR STRING; 3702.AT2G24190.2; -.
DR iPTMnet; Q9ZUH5; -.
DR PaxDb; Q9ZUH5; -.
DR PRIDE; Q9ZUH5; -.
DR ProteomicsDB; 232862; -. [Q9ZUH5-1]
DR EnsemblPlants; AT2G24190.1; AT2G24190.1; AT2G24190. [Q9ZUH5-1]
DR EnsemblPlants; AT2G24190.2; AT2G24190.2; AT2G24190. [Q9ZUH5-2]
DR GeneID; 816953; -.
DR Gramene; AT2G24190.1; AT2G24190.1; AT2G24190. [Q9ZUH5-1]
DR Gramene; AT2G24190.2; AT2G24190.2; AT2G24190. [Q9ZUH5-2]
DR KEGG; ath:AT2G24190; -.
DR Araport; AT2G24190; -.
DR TAIR; locus:2047490; AT2G24190.
DR eggNOG; KOG1208; Eukaryota.
DR HOGENOM; CLU_010194_9_0_1; -.
DR InParanoid; Q9ZUH5; -.
DR OMA; HPNICIN; -.
DR OrthoDB; 1259665at2759; -.
DR PhylomeDB; Q9ZUH5; -.
DR BioCyc; ARA:AT2G24190-MON; -.
DR BioCyc; MetaCyc:AT2G24190-MON; -.
DR SABIO-RK; Q9ZUH5; -.
DR PRO; PR:Q9ZUH5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZUH5; baseline and differential.
DR Genevisible; Q9ZUH5; AT.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0008106; F:alcohol dehydrogenase (NADP+) activity; IDA:TAIR.
DR CDD; cd05324; carb_red_PTCR-like_SDR_c; 1.
DR InterPro; IPR045313; CBR1-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..296
FT /note="Short-chain dehydrogenase/reductase 2b"
FT /id="PRO_0000349099"
FT ACT_SITE 220
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q12634"
FT BINDING 16..40
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q12634"
FT BINDING 164
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q12634"
FT VAR_SEQ 6
FT /note="P -> PSSGLC (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_035197"
SQ SEQUENCE 296 AA; 32850 MW; DE80F12990A325AD CRC64;
MAEESPRYAI VTGGNRGIGF EICRQLANKG IRVILTSRDE KQGLEAVETL KKELEISDQS
IVFHQLDVSD PVSVTSLAEF VKTHFGKLDI LINNAGVGGV ITDVDALRAG TGKEGFKWEE
TITETYELAE ECIKINYYGP KRMCEAFIPL LQLSDSPRII NVSSFMGQVK NLVNEWAKGI
LSDAENLTEV RIDQVINQLL NDLKEDTAKT KYWAKVMSAY VVSKAGLNAY TRILAKKHPE
IRVNSVCPGF VKTDMNFKTG ILSVEEGASS PVRLALLPHQ ESPSGCFFDR KQVSEF
