ID   SDRM_STAAN              Reviewed;         447 AA.
AC   Q99S97;
DT   29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 121.
DE   RecName: Full=Multidrug efflux pump SdrM {ECO:0000303|PubMed:16508166};
GN   Name=sdrM {ECO:0000303|PubMed:16508166};
GN   OrderedLocusNames=SA1972 {ECO:0000312|EMBL:BAB43261.1};
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   FUNCTION IN DRUG RESISTANCE, AND INDUCTION.
RC   STRAIN=N315;
RX   PubMed=16508166; DOI=10.1248/bpb.29.554;
RA   Yamada Y., Hideka K., Shiota S., Kuroda T., Tsuchiya T.;
RT   "Gene cloning and characterization of SdrM, a chromosomally-encoded
RT   multidrug efflux pump, from Staphylococcus aureus.";
RL   Biol. Pharm. Bull. 29:554-556(2006).
CC   -!- FUNCTION: Energy-dependent drug efflux pump that increases resistance
CC       to antimicrobial agents such as norfloxacin, acriflavine and ethidium
CC       bromide. {ECO:0000269|PubMed:16508166}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Not expressed constitutively. {ECO:0000269|PubMed:16508166}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000018; BAB43261.1; -; Genomic_DNA.
DR   PIR; D90012; D90012.
DR   RefSeq; WP_001237506.1; NC_002745.2.
DR   AlphaFoldDB; Q99S97; -.
DR   SMR; Q99S97; -.
DR   TCDB; 2.A.1.3.60; the major facilitator superfamily (mfs).
DR   EnsemblBacteria; BAB43261; BAB43261; BAB43261.
DR   KEGG; sau:SA1972; -.
DR   HOGENOM; CLU_000960_2_5_9; -.
DR   OMA; TALFMEN; -.
DR   Proteomes; UP000000751; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..447
FT                   /note="Multidrug efflux pump SdrM"
FT                   /id="PRO_0000432887"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        161..181
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..237
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        323..342
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..363
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..412
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        418..438
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   447 AA;  49410 MW;  B641B4C5CD9F2F1A CRC64;
     MRLKSIITVI ALILIMFMSA IESSIISLAL PTIKQDLNAG NLISLIFTAY FIALVIANPI
     VGELLSRFKI IYVAIAGLLL FSIGSFMCGL STNFTMLIIS RVIQGFGSGV LMSLSQIVPK
     LAFEIPLRYK IMGIVGSVWG ISSIIGPLLG GGILEFATWH WLFYINIPIA IIAIILVIWT
     FHFPEEETVA KSKFDTKGLT LFYVFIGLIM FALLNQQLLL LNFLSFILAI VVAMCLFKVE
     KHVSSPFLPV VEFNRSITLV FITDLLTAIC LMGFNLYIPV YLQEQLGLSP LQSGLVIFPL
     SVAWITLNFN LHRIEAKLSR KVIYLLSFTL LLVSSIIISF GIKLPVLIAF VLILAGLSFG
     YIYTKDSVIV QEETSPLQMK KMMSFYGLTK NLGASIGSTI MGYLYAIQSG IFGPNLHNVL
     SAVAVISIGL IVLWVVFFKE QSSQSKE