ID   BFPB_ECO11              Reviewed;         552 AA.
AC   Q9S142; Q46777;
DT   30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Outer membrane lipoprotein BfpB;
DE   AltName: Full=Bundle-forming pilus B;
DE   Flags: Precursor;
GN   Name=bfpB;
OS   Escherichia coli O111:H-.
OG   Plasmid pB171.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=168927;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O111:H- / B171 / EPEC;
RX   PubMed=8626330; DOI=10.1128/jb.178.9.2613-2628.1996;
RA   Sohel I., Puente J.L., Ramer S.W., Bieber D., Wu C.-Y., Schoolnik G.K.;
RT   "Enteropathogenic Escherichia coli: identification of a gene cluster coding
RT   for bundle-forming pilus morphogenesis.";
RL   J. Bacteriol. 178:2613-2628(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O111:H- / B171 / EPEC;
RX   PubMed=10496929; DOI=10.1128/iai.67.10.5455-5462.1999;
RA   Tobe T., Hayashi T., Han C.-G., Schoolnik G.K., Ohtsubo E., Sasakawa C.;
RT   "Complete DNA sequence and structural analysis of the enteropathogenic
RT   Escherichia coli adherence factor plasmid.";
RL   Infect. Immun. 67:5455-5462(1999).
RN   [3]
RP   CHARACTERIZATION.
RC   STRAIN=O111:H- / B171 / EPEC;
RX   PubMed=8932312; DOI=10.1128/jb.178.22.6555-6563.1996;
RA   Ramer S.W., Bieber D., Schoolnik G.K.;
RT   "BfpB, an outer membrane lipoprotein required for the biogenesis of bundle-
RT   forming pili in enteropathogenic Escherichia coli.";
RL   J. Bacteriol. 178:6555-6563(1996).
CC   -!- FUNCTION: Is absolutely required for pilus biogenesis, and for EPEC
CC       localized adherence and autoaggregation. Acts at a step in the BFP
CC       biogenic pathway after production and processing of the structural
CC       pilus subunit BfpA.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane; Lipid-anchor.
CC   -!- INDUCTION: During exponential-phase growth; repressed by ammonium.
CC   -!- SIMILARITY: Belongs to the bacterial secretin family. {ECO:0000305}.
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DR   EMBL; U27184; AAC44041.1; -; Genomic_DNA.
DR   EMBL; AB024946; BAA84840.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9S142; -.
DR   TCDB; 1.B.22.7.1; the outer bacterial membrane secretin (secretin) family.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009297; P:pilus assembly; IEA:InterPro.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   InterPro; IPR011514; Secretin_N_2.
DR   InterPro; IPR004846; T2SS/T3SS.
DR   Pfam; PF00263; Secretin; 1.
DR   Pfam; PF07655; Secretin_N_2; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Fimbrium biogenesis; Lipoprotein; Membrane; Palmitate;
KW   Plasmid; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000305"
FT   CHAIN           18..552
FT                   /note="Outer membrane lipoprotein BfpB"
FT                   /id="PRO_0000020806"
FT   REGION          218..244
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           18
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           18
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        332..335
FT                   /note="LLKH -> FVND (in Ref. 1; AAC44041)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   552 AA;  58372 MW;  F8CAE36171DCA956 CRC64;
     MKLGRYSLFL LCPLLASCSG NGFYKDNLGV IDKNILHADT SLLKSKNKEH YKSSDMVSKT
     DSIYIGNSSF QTYHGEPLPG KLEGVHGIIL RSSTPLGFDE VLSMIQDSSG IPIVKHTTKD
     VISGGVSSKS LAATVAEKMN SATGGKSTDQ FDHLLLEVSS EHQLMDVNYQ GALSTFLDKV
     AANYNLYWTY ESGRIAFSNE ETKRFSISIL PGGKYTSKNS ISSDSNSSSG SSGSSGSSSS
     DSGAELKFDS DVDFWKDIEN SIKLILGSDG SYSISTSTSS VIVRTSSANM KKINEYINTL
     NAQLERQVTI DVAIYNVTTT DSSDLAMSLE ALLKHNGGVL GSVSTSNFAA TSGTPSFTGY
     LNGNGDSSNQ VLLNLLAEKG KVSVVTSASV TTMSGQPVPL KVGNDRTYVS EIGTVLSQSS
     TSTTASTSTV TSGFLMNLLP QVADDGNILL QYGVTLSELV GSNNGFDQAT VNGTVIQLPN
     VDSTTFVQSS MLRNGNTLVL AGYEKKRNES VDQGVGTTSF KLLGGALNGS ASRTVTVICI
     TPRIIDLKAS GE