ID   BFPB_ECO27              Reviewed;         552 AA.
AC   Q47068; B7UTD3;
DT   30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Outer membrane lipoprotein BfpB;
DE   AltName: Full=Bundle-forming pilus B;
DE   Flags: Precursor;
GN   Name=bfpB; OrderedLocusNames=E2348_P1_005;
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OG   Plasmid pMAR2.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8733231; DOI=10.1111/j.1365-2958.1996.tb02620.x;
RA   Stone K.D., Zhang H., Carlson L.K., Donnenberg M.S.;
RT   "A cluster of fourteen genes from enteropathogenic Escherichia coli is
RT   sufficient for the biogenesis of a type IV pilus.";
RL   Mol. Microbiol. 20:325-337(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=18952797; DOI=10.1128/jb.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R.,
RA   Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A.,
RA   Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Is absolutely required for pilus biogenesis, and for EPEC
CC       localized adherence and autoaggregation. Acts at a step in the BFP
CC       biogenic pathway after production and processing of the structural
CC       pilus subunit BfpA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the bacterial secretin family. {ECO:0000305}.
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DR   EMBL; Z68186; CAA92328.1; -; Genomic_DNA.
DR   EMBL; FM180569; CAS07441.1; -; Genomic_DNA.
DR   PIR; S70968; S70968.
DR   RefSeq; WP_000766020.1; NC_011603.1.
DR   AlphaFoldDB; Q47068; -.
DR   EnsemblBacteria; CAS07441; CAS07441; E2348_P1_005.
DR   KEGG; ecg:E2348_P1_005; -.
DR   HOGENOM; CLU_015882_0_0_6; -.
DR   OMA; FYYLDTR; -.
DR   Proteomes; UP000008205; Plasmid pMAR2.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009297; P:pilus assembly; IEA:InterPro.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   InterPro; IPR011514; Secretin_N_2.
DR   InterPro; IPR004846; T2SS/T3SS.
DR   Pfam; PF00263; Secretin; 1.
DR   Pfam; PF07655; Secretin_N_2; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Fimbrium biogenesis; Lipoprotein; Membrane; Palmitate;
KW   Plasmid; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           18..552
FT                   /note="Outer membrane lipoprotein BfpB"
FT                   /id="PRO_0000020807"
FT   REGION          218..244
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           18
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           18
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CONFLICT        185..189
FT                   /note="NLYWT -> KPVLGQ (in Ref. 1; CAA92328)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   552 AA;  58372 MW;  F8CAE36171DCA956 CRC64;
     MKLGRYSLFL LCPLLASCSG NGFYKDNLGV IDKNILHADT SLLKSKNKEH YKSSDMVSKT
     DSIYIGNSSF QTYHGEPLPG KLEGVHGIIL RSSTPLGFDE VLSMIQDSSG IPIVKHTTKD
     VISGGVSSKS LAATVAEKMN SATGGKSTDQ FDHLLLEVSS EHQLMDVNYQ GALSTFLDKV
     AANYNLYWTY ESGRIAFSNE ETKRFSISIL PGGKYTSKNS ISSDSNSSSG SSGSSGSSSS
     DSGAELKFDS DVDFWKDIEN SIKLILGSDG SYSISTSTSS VIVRTSSANM KKINEYINTL
     NAQLERQVTI DVAIYNVTTT DSSDLAMSLE ALLKHNGGVL GSVSTSNFAA TSGTPSFTGY
     LNGNGDSSNQ VLLNLLAEKG KVSVVTSASV TTMSGQPVPL KVGNDRTYVS EIGTVLSQSS
     TSTTASTSTV TSGFLMNLLP QVADDGNILL QYGVTLSELV GSNNGFDQAT VNGTVIQLPN
     VDSTTFVQSS MLRNGNTLVL AGYEKKRNES VDQGVGTTSF KLLGGALNGS ASRTVTVICI
     TPRIIDLKAS GE