ID   BFP_ALIFS               Reviewed;         200 AA.
AC   P80893; B6F211;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   16-MAR-2016, sequence version 2.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Blue fluorescence protein {ECO:0000303|PubMed:9183020};
DE            Short=BFP {ECO:0000303|PubMed:9183020};
GN   Name=Y1-BFP {ECO:0000312|EMBL:BAG71497.1};
OS   Aliivibrio fischeri (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33715 / Y-1;
RA   Karatani H., Osaki T., Yasui M., Ohta S.;
RT   "Gene analysis of a blue fluorescent protein from yellow emitting Vibrio
RT   fischeri strain Y1 and the protein expression.";
RL   ITE Lett. Batteries New Technol. Med. 7:477-483(2006).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-52, FUNCTION, AND SUBUNIT.
RC   STRAIN=ATCC 33715 / Y-1;
RX   PubMed=9183020; DOI=10.1111/j.1432-1033.1997.00790.x;
RA   Petushkov V.N., Lee J.;
RT   "Purification and characterization of flavoproteins and cytochromes from
RT   the yellow bioluminescence marine bacterium Vibrio fischeri strain Y1.";
RL   Eur. J. Biochem. 245:790-796(1997).
CC   -!- FUNCTION: Blue fluorescence protein (BFP) that can bind 6,7-dimethyl-8-
CC       ribityllumazine, riboflavin, and 6-methyl-7-oxo-8-ribityllumazine as a
CC       bound fluorophore. Has no riboflavin-synthase activity.
CC       {ECO:0000269|PubMed:9183020}.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|Ref.1}.
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DR   EMBL; AB459501; BAG71497.1; -; Genomic_DNA.
DR   AlphaFoldDB; P80893; -.
DR   SMR; P80893; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.30.20; -; 2.
DR   InterPro; IPR023366; ATP_synth_asu-like_sf.
DR   InterPro; IPR001783; Lumazine-bd.
DR   InterPro; IPR026017; Lumazine-bd_dom.
DR   InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR   PANTHER; PTHR21098; PTHR21098; 1.
DR   Pfam; PF00677; Lum_binding; 2.
DR   PIRSF; PIRSF000498; Riboflavin_syn_A; 1.
DR   SUPFAM; SSF63380; SSF63380; 2.
DR   PROSITE; PS51177; LUMAZINE_BIND; 2.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Luminescence; Repeat.
FT   CHAIN           1..200
FT                   /note="Blue fluorescence protein"
FT                   /id="PRO_0000068155"
FT   REPEAT          1..111
FT                   /note="Lumazine-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00524"
FT   REPEAT          112..200
FT                   /note="Lumazine-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00524"
SQ   SEQUENCE   200 AA;  22215 MW;  4383B878A1AAD1B2 CRC64;
     MFKGNVQGVG TVENIDKGAK FQSLHGVSLL PIDADLQSHD IIFPEDILEG VTSGELIAIN
     GVRLTVVHTD KSIVRFDIND ALELTTLGQL KVGDKVNIEK SFKFGDMTGG RSLSGIVTGV
     ADIVEFIEKE NNRQIWIEAP EHLTEFLVEK KYIGVDGVYL VIDAIENNRF CINLLLETDM
     RWYKKGSKVN IEIPDIAGNW