SEBP2_ARATH
ID SEBP2_ARATH Reviewed; 487 AA.
AC Q93WN0; O23265; Q8L7F3; Q9ASS5;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Selenium-binding protein 2;
DE AltName: Full=Protein EMBRYO SAC DEVELOPMENT ARREST 38;
GN Name=SBP2; Synonyms=EDA38; OrderedLocusNames=At4g14040;
GN ORFNames=dl3061c, FCAALL.29;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15634699; DOI=10.1242/dev.01595;
RA Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S.,
RA Capron A., Xie L.-F., Ye D., Sundaresan V.;
RT "Genetic and molecular identification of genes required for female
RT gametophyte development and function in Arabidopsis.";
RL Development 132:603-614(2005).
RN [6]
RP TISSUE SPECIFICITY, AND INDUCTION BY CADMIUM.
RX PubMed=18354042; DOI=10.1104/pp.107.114033;
RA Dutilleul C., Jourdain A., Bourguignon J., Hugouvieux V.;
RT "The Arabidopsis putative selenium-binding protein family: expression study
RT and characterization of SBP1 as a potential new player in cadmium
RT detoxification processes.";
RL Plant Physiol. 147:239-251(2008).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Required for the fusion of female gametophyte polar nuclei.
CC {ECO:0000269|PubMed:15634699}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in seedlings, leaves and stems,
CC and, to a lower extent, in flowers and roots.
CC {ECO:0000269|PubMed:18354042}.
CC -!- INDUCTION: Slightly induced in roots by cadmium.
CC {ECO:0000269|PubMed:18354042}.
CC -!- DISRUPTION PHENOTYPE: Impaired fusion of polar nuclei.
CC {ECO:0000269|PubMed:15634699}.
CC -!- SIMILARITY: Belongs to the selenium-binding protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB46000.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78446.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z97335; CAB46000.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161537; CAB78446.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83364.1; -; Genomic_DNA.
DR EMBL; AF367311; AAK32898.1; -; mRNA.
DR EMBL; AF370130; AAK43945.1; -; mRNA.
DR EMBL; AY040046; AAK64104.1; -; mRNA.
DR EMBL; AY065104; AAL38280.1; -; mRNA.
DR EMBL; AY065108; AAL38284.1; -; mRNA.
DR EMBL; AY133613; AAM91443.1; -; mRNA.
DR EMBL; AY136288; AAM96954.1; -; mRNA.
DR EMBL; BT000385; AAN15704.1; -; mRNA.
DR EMBL; BT000406; AAN15725.1; -; mRNA.
DR PIR; A85153; A85153.
DR RefSeq; NP_193140.1; NM_117479.4.
DR AlphaFoldDB; Q93WN0; -.
DR SMR; Q93WN0; -.
DR BioGRID; 12337; 3.
DR IntAct; Q93WN0; 1.
DR STRING; 3702.AT4G14040.1; -.
DR iPTMnet; Q93WN0; -.
DR MetOSite; Q93WN0; -.
DR PaxDb; Q93WN0; -.
DR PRIDE; Q93WN0; -.
DR ProteomicsDB; 232860; -.
DR EnsemblPlants; AT4G14040.1; AT4G14040.1; AT4G14040.
DR GeneID; 827040; -.
DR Gramene; AT4G14040.1; AT4G14040.1; AT4G14040.
DR KEGG; ath:AT4G14040; -.
DR Araport; AT4G14040; -.
DR TAIR; locus:2129356; AT4G14040.
DR eggNOG; KOG0918; Eukaryota.
DR HOGENOM; CLU_032512_0_0_1; -.
DR InParanoid; Q93WN0; -.
DR OMA; DETCQSP; -.
DR OrthoDB; 483905at2759; -.
DR PhylomeDB; Q93WN0; -.
DR PRO; PR:Q93WN0; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q93WN0; baseline and differential.
DR Genevisible; Q93WN0; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0008430; F:selenium binding; IEA:InterPro.
DR GO; GO:0010197; P:polar nucleus fusion; IMP:TAIR.
DR InterPro; IPR008826; Se-bd.
DR PANTHER; PTHR23300; PTHR23300; 1.
DR Pfam; PF05694; SBP56; 1.
PE 1: Evidence at protein level;
KW Acetylation; Reference proteome; Selenium.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..487
FT /note="Selenium-binding protein 2"
FT /id="PRO_0000403644"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CONFLICT 2
FT /note="A -> V (in Ref. 4; AAK32898/AAM91443)"
FT /evidence="ECO:0000305"
FT CONFLICT 248
FT /note="P -> A (in Ref. 4; AAM96954/AAN15725)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 487 AA; 53937 MW; 0567C4138EF78A48 CRC64;
MATETVLATA VSNGKSKGCC KSGPGYATPL AAMAGPREKL IYVTALYSGT GRDKPDYLAT
VDVDPSSPTF SSVIHRLKMP YIGDELHHTG WNSCSSCHGD ASADRRYLVL PGLISGRIYA
IDTKTDPKAP SLYKVVEPKE IAEKTGLAFP HTSHCLASGD MLVSCLGDKE GNAKGNGFLL
LDSDFNVKSR WDKPGHGPLF GYDFWYQPRF KTMISTSWGA PKAFSKGFNL QHVADGLYGS
HLHIYQWPEG EMKQIIDLGN TGLLPLEIRF LHDPSKDTGY VGSALSSNMI RFFKNSDDTW
SHEVVISVKP LKVENWILPE MPGLITDFLI SLDDRFFYFV NWLHGDIRQY NIEDPKNPVL
TGQIWVGGLL QKGSPYKAVG EDGNTYQFDV PQIKGKSLRA GPQMIQLSLD GKRLYATNSL
FSAWDRQFYP EIMEKGSHII QIDVDTDKGG LTLNPDFFVD FGDEPDGPAL AHEMRYPGGD
CTSDIWI
