ID   SEC12_CANGA             Reviewed;         459 AA.
AC   Q6FIY2;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Guanine nucleotide-exchange factor SEC12;
DE   AltName: Full=Protein transport protein SEC12;
GN   Name=SEC12; OrderedLocusNames=CAGL0M10703g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Guanine nucleotide-exchange factor (GEF) required for the
CC       formation or budding of transport vesicles from the ER. This function
CC       involves the cytoplasmic domain of the protein, which is thought to
CC       interact with the small GTP-binding protein SAR1. Required for
CC       autophagy (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type II membrane protein {ECO:0000250}. Golgi apparatus,
CC       cis-Golgi network membrane {ECO:0000250}; Single-pass type II membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat SEC12 family. {ECO:0000305}.
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DR   EMBL; CR380959; CAG62790.1; -; Genomic_DNA.
DR   RefSeq; XP_449812.1; XM_449812.1.
DR   AlphaFoldDB; Q6FIY2; -.
DR   SMR; Q6FIY2; -.
DR   STRING; 5478.XP_449812.1; -.
DR   EnsemblFungi; CAG62790; CAG62790; CAGL0M10703g.
DR   GeneID; 2891377; -.
DR   KEGG; cgr:CAGL0M10703g; -.
DR   CGD; CAL0137173; CAGL0M10703g.
DR   VEuPathDB; FungiDB:CAGL0M10703g; -.
DR   eggNOG; KOG0771; Eukaryota.
DR   HOGENOM; CLU_033006_0_0_1; -.
DR   InParanoid; Q6FIY2; -.
DR   OMA; NEKESHT; -.
DR   Proteomes; UP000002428; Chromosome M.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:EnsemblFungi.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0003400; P:regulation of COPII vesicle coating; IEA:EnsemblFungi.
DR   GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR045260; Sec12-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PANTHER; PTHR23284; PTHR23284; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; ER-Golgi transport; Glycoprotein; Golgi apparatus;
KW   GTPase activation; Membrane; Protein transport; Reference proteome; Repeat;
KW   Signal-anchor; Transmembrane; Transmembrane helix; Transport; WD repeat.
FT   CHAIN           1..459
FT                   /note="Guanine nucleotide-exchange factor SEC12"
FT                   /id="PRO_0000295526"
FT   TOPO_DOM        1..351
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        352..370
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        371..459
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   REPEAT          256..291
FT                   /note="WD 1"
FT   REPEAT          294..334
FT                   /note="WD 2"
FT   REGION          417..459
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        417..437
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        435
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   459 AA;  50383 MW;  D93B2360677EBBFB CRC64;
     MKFHTKLYKV GYPLYGAKFV CDDQFVVTGG GGEGNNGVDN KLTVLKVGSS EGNGLRVDEV
     CDATLPANDD SPTALDAVGD TILIGCNENS AKVKSGAGNS HVRKFRYDGK SLKFESAADI
     DKSTNPEDYT KVIRLSKDGS EAAIASSKNP PSMAIIDPRN YSVKYEIETG RDVKDLHFSP
     NGKLIGYITE SSLEIISTVT GSCVVRKTDF DRKIILSKMK FLDDNNVLIA ATWASSKGIL
     LTKISIKSGK TTVFWSRQIS SKFKGITAMD VNDQMNLVML ATNDNSVLLV KLRNLSVGKT
     FTQVHGFAIT RVIFSPDSRY AVSISAAETV HVIEIPSDFA DSRSLSESTT QWLMNTIMVL
     FLAFVLNSMY KEDMHKNVLN YFRSVKSIDS SSILSMEDME QVTLVGTIST SVRSTTQRFE
     SSKSVSSTSV VARTNVSEKE RSQQDPVKKS PEQHQQNQQ