SEC1A_ARATH
ID SEC1A_ARATH Reviewed; 673 AA.
AC Q9C5P7; O23672; Q9LPC0;
DT 01-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 3.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Protein transport Sec1a;
DE Short=AtSec1a;
GN Name=SEC1A; OrderedLocusNames=At1g02010; ORFNames=F22M8.14, T7I23.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RC TISSUE=Flower;
RX PubMed=11157980; DOI=10.1083/jcb.152.3.531;
RA Assaad F.F., Huet Y., Mayer U., Juergens G.;
RT "The cytokinesis gene KEULE encodes a Sec1 protein that binds the syntaxin
RT KNOLLE.";
RL J. Cell Biol. 152:531-544(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
CC -!- FUNCTION: Involved in the vesicle trafficking. Binds syntaxins (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Does not bind the syntaxin KNOLLE.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9C5P7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9C5P7-2; Sequence=VSP_036638, VSP_036640;
CC Name=3;
CC IsoId=Q9C5P7-3; Sequence=VSP_036635, VSP_036636, VSP_036637,
CC VSP_036639;
CC -!- MISCELLANEOUS: [Isoform 3]: Incomplete sequence. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the STXBP/unc-18/SEC1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC24365.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF76479.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF335539; AAK15767.1; -; mRNA.
DR EMBL; AC020622; AAF76479.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U89959; AAC24365.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27365.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM57903.1; -; Genomic_DNA.
DR EMBL; BX816413; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; B86152; B86152.
DR RefSeq; NP_001320381.1; NM_001331318.1. [Q9C5P7-2]
DR RefSeq; NP_563643.4; NM_100081.5. [Q9C5P7-1]
DR AlphaFoldDB; Q9C5P7; -.
DR SMR; Q9C5P7; -.
DR BioGRID; 24523; 1.
DR STRING; 3702.AT1G02010.1; -.
DR iPTMnet; Q9C5P7; -.
DR PaxDb; Q9C5P7; -.
DR ProteomicsDB; 232756; -. [Q9C5P7-1]
DR EnsemblPlants; AT1G02010.1; AT1G02010.1; AT1G02010. [Q9C5P7-1]
DR EnsemblPlants; AT1G02010.3; AT1G02010.3; AT1G02010. [Q9C5P7-2]
DR GeneID; 839288; -.
DR Gramene; AT1G02010.1; AT1G02010.1; AT1G02010. [Q9C5P7-1]
DR Gramene; AT1G02010.3; AT1G02010.3; AT1G02010. [Q9C5P7-2]
DR KEGG; ath:AT1G02010; -.
DR Araport; AT1G02010; -.
DR TAIR; locus:2025482; AT1G02010.
DR eggNOG; KOG1300; Eukaryota.
DR HOGENOM; CLU_009210_3_0_1; -.
DR InParanoid; Q9C5P7; -.
DR OMA; SKGPMET; -.
DR PhylomeDB; Q9C5P7; -.
DR PRO; PR:Q9C5P7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C5P7; baseline and differential.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030141; C:secretory granule; IBA:GO_Central.
DR GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.1910; -; 2.
DR Gene3D; 3.40.50.2060; -; 1.
DR Gene3D; 3.90.830.10; -; 1.
DR InterPro; IPR043154; Sec-1-like_dom1.
DR InterPro; IPR043127; Sec-1-like_dom3a.
DR InterPro; IPR001619; Sec1-like.
DR InterPro; IPR027482; Sec1-like_dom2.
DR InterPro; IPR036045; Sec1-like_sf.
DR PANTHER; PTHR11679; PTHR11679; 1.
DR Pfam; PF00995; Sec1; 1.
DR PIRSF; PIRSF005715; VPS45_Sec1; 1.
DR SUPFAM; SSF56815; SSF56815; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Protein transport; Reference proteome; Transport.
FT CHAIN 1..673
FT /note="Protein transport Sec1a"
FT /id="PRO_0000206295"
FT REGION 538..591
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..591
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 74..101
FT /note="LVEELFKRREPMPGMDAIYFIQPSKENI -> C (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:11157980"
FT /id="VSP_036635"
FT VAR_SEQ 150..160
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:11157980"
FT /id="VSP_036636"
FT VAR_SEQ 452..461
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:11157980"
FT /id="VSP_036637"
FT VAR_SEQ 590..611
FT /note="SDSVLKSASTEFKKLGQRIFVF -> RHSILHNDFLFFGPFERSLDFF (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_036638"
FT VAR_SEQ 590..606
FT /note="SDSVLKSASTEFKKLGQ -> RHSILHNDFLFFGPFE (in isoform
FT 3)"
FT /evidence="ECO:0000303|PubMed:11157980"
FT /id="VSP_036639"
FT VAR_SEQ 612..673
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_036640"
FT CONFLICT 102
FT /note="V -> F (in Ref. 4; BX816413)"
FT /evidence="ECO:0000305"
FT CONFLICT 610
FT /note="V -> L (in Ref. 4; BX816413)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 673 AA; 76108 MW; 34DD4C00816A75D7 CRC64;
MSFSDSESSS HGGGGGDYKF FRQISRDRLL HEMLGSTKTG DSKAWKILIM DRVTVKVMSQ
SCKMADITDQ GISLVEELFK RREPMPGMDA IYFIQPSKEN IVMFLSDMSG REPLYRKAFI
FFSSTIPKEL VNHIKSDSSV LPRIGALREM NMEYFPIDNQ GFLTDHEQAL ETLYAEDAEN
SRHFHICLNI MATRIATVFA SLKELPFVRY RAAKSTASRD LVPSKLAAAI WDCISKYKAI
PNFPQTETCE LLIVDRSVDQ IAPIIHEWTY DAMCHDLLDM EGNKHVIEVP SKTGGPPEKK
EIVLEDHDPV WLELRHTHIA DASERLHEKM TNFASKNKAA QMRSRDGSEL STRDLQKIVQ
ALPQYGEQVD KLSTHVELAG KINRIIRDTG LRDLGQLEQD LVFGDAGAKD VINFLRTNQD
TNPENKLRLL MIYATVYPEK FEGDKGVKLM QLARLSPVDM KVISNMQLIA GSPENKAKSG
SFSLKFDAGK TKQANRKDRS GEEETWQLFR FYPMIEELLE KLVKGDLSKS DYLCMNQSSH
KEESEARTGS VRKSSAPTAV PERKATPHSM RSRRTATWAR PHSSDDGYSS DSVLKSASTE
FKKLGQRIFV FIIGGATRSE LRVCHKLTSS LRREVVLGST SFDDPPQYIT KLKLLSEKDI
QGAPAQPFKP QYW
