SEC20_RAT
ID SEC20_RAT Reviewed; 228 AA.
AC Q8VHI8;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Vesicle transport protein SEC20;
GN Name=Bnip1 {ECO:0000312|EMBL:AAL50991.1, ECO:0000312|RGD:620799};
GN Synonyms=Sec20l;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000312|EMBL:AAL50991.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley {ECO:0000312|EMBL:AAL50991.1};
RA Itoh T., Itoh A., Pleasure D.;
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: As part of a SNARE complex may be involved in endoplasmic
CC reticulum membranes fusion and be required for the maintenance of
CC endoplasmic reticulum organization. Also plays a role in apoptosis. It
CC is for instance required for endoplasmic reticulum stress-induced
CC apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also
CC be involved in mitochondrial autophagy. {ECO:0000250|UniProtKB:Q12981}.
CC -!- SUBUNIT: Component of a SNARE complex consisting of STX18, USE1L,
CC BNIP1/SEC20L and SEC22B. Interacts directly with STX18, RINT1/TIP20L
CC and NAPA. Interacts with ZW10 through RINT1. Interacts with BCL2.
CC Interacts with RNF186. Interacts with RNF185. Interacts with SQSTM1;
CC increased by 'Lys-63'-linked polyubiquitination of BNIP1.
CC {ECO:0000250|UniProtKB:Q12981}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q12981}; Single-pass type IV membrane protein
CC {ECO:0000255}. Mitochondrion membrane {ECO:0000250|UniProtKB:Q12981};
CC Single-pass type IV membrane protein {ECO:0000255}. Note=Localization
CC to the mitochondrion is regulated by RNF186.
CC {ECO:0000250|UniProtKB:Q12981}.
CC -!- PTM: Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185
CC increases the interaction with the autophagy receptor SQSTM1. Undergoes
CC 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may
CC regulate BNIP1 localization to the mitochondrion.
CC {ECO:0000250|UniProtKB:Q12981}.
CC -!- SIMILARITY: Belongs to the SEC20 family. {ECO:0000305}.
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DR EMBL; AF454391; AAL50991.1; -; mRNA.
DR RefSeq; NP_543173.1; NM_080897.1.
DR AlphaFoldDB; Q8VHI8; -.
DR SMR; Q8VHI8; -.
DR IntAct; Q8VHI8; 3.
DR STRING; 10116.ENSRNOP00000028173; -.
DR PhosphoSitePlus; Q8VHI8; -.
DR jPOST; Q8VHI8; -.
DR PaxDb; Q8VHI8; -.
DR PRIDE; Q8VHI8; -.
DR GeneID; 140932; -.
DR KEGG; rno:140932; -.
DR UCSC; RGD:620799; rat.
DR CTD; 662; -.
DR RGD; 620799; Bnip1.
DR VEuPathDB; HostDB:ENSRNOG00000020753; -.
DR eggNOG; ENOG502QUTT; Eukaryota.
DR HOGENOM; CLU_105902_0_0_1; -.
DR InParanoid; Q8VHI8; -.
DR OMA; DDWARDT; -.
DR OrthoDB; 1328108at2759; -.
DR PhylomeDB; Q8VHI8; -.
DR TreeFam; TF324339; -.
DR Reactome; R-RNO-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR PRO; PR:Q8VHI8; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000020753; Expressed in quadriceps femoris and 20 other tissues.
DR Genevisible; Q8VHI8; RN.
DR GO; GO:0030137; C:COPI-coated vesicle; IDA:RGD.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:HGNC-UCL.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISS:UniProtKB.
DR GO; GO:0031966; C:mitochondrial membrane; ISO:RGD.
DR GO; GO:0005635; C:nuclear envelope; ISS:UniProtKB.
DR GO; GO:0031201; C:SNARE complex; IDA:RGD.
DR GO; GO:0005484; F:SNAP receptor activity; ISO:RGD.
DR GO; GO:0006915; P:apoptotic process; IEP:RGD.
DR GO; GO:0016320; P:endoplasmic reticulum membrane fusion; ISS:HGNC-UCL.
DR GO; GO:0007029; P:endoplasmic reticulum organization; ISS:HGNC-UCL.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0014823; P:response to activity; IEP:RGD.
DR GO; GO:0090649; P:response to oxygen-glucose deprivation; IEP:RGD.
DR GO; GO:0042594; P:response to starvation; IEP:RGD.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR InterPro; IPR005606; Sec20.
DR PANTHER; PTHR12825; PTHR12825; 1.
DR Pfam; PF03908; Sec20; 1.
PE 2: Evidence at transcript level;
KW Apoptosis; Coiled coil; Endoplasmic reticulum; ER-Golgi transport;
KW Membrane; Mitochondrion; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Ubl conjugation.
FT CHAIN 1..228
FT /note="Vesicle transport protein SEC20"
FT /id="PRO_0000232418"
FT TOPO_DOM 1..199
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..228
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT COILED 37..90
FT /evidence="ECO:0000255"
SQ SEQUENCE 228 AA; 26170 MW; 5F2CFED85E18824E CRC64;
MAAPQDVHVR ICNQEIVKFD LEVKALIQDI RDCSGPLSEL TELNTKVKEK FQQLKHRIQE
LEQSAKEQDK ESEKQLLLQE VENHKKQMLS NQTSWRKANL TCKLAIDNLE KAELLQGGDS
LRQRKTTKES LAQTSSTITE SLMGISRMMS QQVQQSEEAM QTLVSSSRTL LDANEEFKSM
SGTIQLGRKL ITKYNRRELT DKLLIFLALA LFLATVLYIV KKRLFPFL
