SEC20_SCHPO
ID SEC20_SCHPO Reviewed; 226 AA.
AC O42852;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Protein transport protein sec20;
GN Name=sec20 {ECO:0000250|UniProtKB:P28791}; ORFNames=SPAC23A1.15c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: SNARE required for targeting and fusion of Golgi-derived
CC retrograde transport vesicles with the ER.
CC {ECO:0000250|UniProtKB:P28791}.
CC -!- SUBUNIT: Component of a SNARE complex consisting of ufe1, sec20, sec22
CC and use1. Interacts with tip20 through its cytoplasmic domain (By
CC similarity). {ECO:0000250|UniProtKB:P28791}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass type IV membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SEC20 family. {ECO:0000305}.
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DR EMBL; CU329670; CAA16989.1; -; Genomic_DNA.
DR PIR; T38234; T38234.
DR RefSeq; NP_594444.1; NM_001019873.2.
DR AlphaFoldDB; O42852; -.
DR SMR; O42852; -.
DR BioGRID; 278480; 2.
DR IntAct; O42852; 1.
DR STRING; 4896.SPAC23A1.15c.1; -.
DR iPTMnet; O42852; -.
DR MaxQB; O42852; -.
DR PaxDb; O42852; -.
DR PRIDE; O42852; -.
DR EnsemblFungi; SPAC23A1.15c.1; SPAC23A1.15c.1:pep; SPAC23A1.15c.
DR GeneID; 2541996; -.
DR KEGG; spo:SPAC23A1.15c; -.
DR PomBase; SPAC23A1.15c; sec20.
DR VEuPathDB; FungiDB:SPAC23A1.15c; -.
DR eggNOG; ENOG502S7WD; Eukaryota.
DR HOGENOM; CLU_1220305_0_0_1; -.
DR InParanoid; O42852; -.
DR OMA; VQAVDMS; -.
DR Reactome; R-SPO-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR PRO; PR:O42852; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:PomBase.
DR GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR GO; GO:0005484; F:SNAP receptor activity; ISS:PomBase.
DR GO; GO:0006886; P:intracellular protein transport; NAS:PomBase.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; ISS:PomBase.
DR InterPro; IPR005606; Sec20.
DR PANTHER; PTHR12825; PTHR12825; 1.
DR Pfam; PF03908; Sec20; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endoplasmic reticulum; ER-Golgi transport; Membrane;
KW Phosphoprotein; Protein transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..226
FT /note="Protein transport protein sec20"
FT /id="PRO_0000232410"
FT TOPO_DOM 1..189
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..226
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT COILED 53..75
FT /evidence="ECO:0000255"
FT MOD_RES 97
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 226 AA; 26289 MW; 3520956D51618941 CRC64;
MADVLNALEE KVVELQQSES VEVIKRHFRE FRKIWETARV ELEYSSIQLD SVLRYEKAVQ
EYIRLNRRYR NKIASGEPWL PIAQEIGKIV DEEEITSPSD GSLQKRSMDN SGSWQSDDIY
LTSASQVTAA MRDIHAQMVQ AVDMSAENAM ELSSSTNLLE TLQEKYFGVE DVLYTSKRII
KSLKLSDRSD YFLVVSGFGF FIFVVVYLLF KRIVWPILSM FLWFLR
