SEC20_YEAST
ID SEC20_YEAST Reviewed; 383 AA.
AC P28791; D6VTC0;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Protein transport protein SEC20;
GN Name=SEC20; OrderedLocusNames=YDR498C; ORFNames=D9719.4;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=1537327; DOI=10.1002/j.1460-2075.1992.tb05071.x;
RA Sweet D.J., Pelham H.R.B.;
RT "The Saccharomyces cerevisiae SEC20 gene encodes a membrane glycoprotein
RT which is sorted by the HDEL retrieval system.";
RL EMBO J. 11:423-432(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION, AND INTERACTION WITH UFE1; SEC22 AND USE1.
RX PubMed=12853481; DOI=10.1093/emboj/cdg339;
RA Dilcher M., Veith B., Chidambaram S., Hartmann E., Schmitt H.D.,
RA Fischer von Mollard G.;
RT "Use1p is a yeast SNARE protein required for retrograde traffic to the
RT ER.";
RL EMBO J. 22:3664-3674(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: SNARE required for targeting and fusion of Golgi-derived
CC retrograde transport vesicles with the ER.
CC {ECO:0000269|PubMed:12853481}.
CC -!- SUBUNIT: Component of a SNARE complex consisting of UFE1, USE1, SEC20
CC and SEC22 or YKT6. Interacts with TIP20 through its cytoplasmic domain.
CC {ECO:0000269|PubMed:12853481}.
CC -!- INTERACTION:
CC P28791; P22214: SEC22; NbExp=4; IntAct=EBI-16572, EBI-16577;
CC P28791; P33891: TIP20; NbExp=8; IntAct=EBI-16572, EBI-19396;
CC P28791; P41834: UFE1; NbExp=5; IntAct=EBI-16572, EBI-20016;
CC P28791; P53146: USE1; NbExp=4; IntAct=EBI-16572, EBI-23881;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000255|PROSITE-ProRule:PRU10138, ECO:0000269|PubMed:1537327};
CC Single-pass type IV membrane protein {ECO:0000269|PubMed:1537327}.
CC -!- PTM: O-glycosylated, but not N-glycosylated. {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 4910 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SEC20 family. {ECO:0000305}.
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DR EMBL; X60215; CAA42776.1; -; Genomic_DNA.
DR EMBL; U33057; AAB64940.1; -; Genomic_DNA.
DR EMBL; AY693133; AAT93152.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12330.1; -; Genomic_DNA.
DR PIR; S19633; S19633.
DR RefSeq; NP_010786.3; NM_001180806.3.
DR AlphaFoldDB; P28791; -.
DR SMR; P28791; -.
DR BioGRID; 32549; 421.
DR ComplexPortal; CPX-5304; Endoplasmic reticulum snare complex UFE1-USE1-SEC20-SEC22.
DR DIP; DIP-5844N; -.
DR IntAct; P28791; 15.
DR MINT; P28791; -.
DR STRING; 4932.YDR498C; -.
DR iPTMnet; P28791; -.
DR MaxQB; P28791; -.
DR PaxDb; P28791; -.
DR PRIDE; P28791; -.
DR TopDownProteomics; P28791; -.
DR EnsemblFungi; YDR498C_mRNA; YDR498C; YDR498C.
DR GeneID; 852109; -.
DR KEGG; sce:YDR498C; -.
DR SGD; S000002906; SEC20.
DR VEuPathDB; FungiDB:YDR498C; -.
DR eggNOG; ENOG502S7WD; Eukaryota.
DR GeneTree; ENSGT00390000014412; -.
DR HOGENOM; CLU_046734_0_0_1; -.
DR InParanoid; P28791; -.
DR OMA; TAIETHE; -.
DR BioCyc; YEAST:G3O-30021-MON; -.
DR Reactome; R-SCE-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR PRO; PR:P28791; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P28791; protein.
DR GO; GO:0098554; C:cytoplasmic side of endoplasmic reticulum membrane; IC:ComplexPortal.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR GO; GO:0012508; C:Golgi to ER transport vesicle membrane; IC:ComplexPortal.
DR GO; GO:0016021; C:integral component of membrane; IDA:SGD.
DR GO; GO:0031201; C:SNARE complex; IDA:SGD.
DR GO; GO:0005484; F:SNAP receptor activity; ISM:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IMP:SGD.
DR GO; GO:0048279; P:vesicle fusion with endoplasmic reticulum; IC:SGD.
DR InterPro; IPR005606; Sec20.
DR PANTHER; PTHR12825; PTHR12825; 1.
DR Pfam; PF03908; Sec20; 1.
DR PROSITE; PS00014; ER_TARGET; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endoplasmic reticulum; ER-Golgi transport; Glycoprotein;
KW Membrane; Protein transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..383
FT /note="Protein transport protein SEC20"
FT /id="PRO_0000097659"
FT TOPO_DOM 1..275
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..292
FT /note="Helical; Anchor for type IV membrane protein"
FT TOPO_DOM 293..383
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT COILED 6..26
FT /evidence="ECO:0000255"
FT MOTIF 380..383
FT /note="Prevents secretion from ER"
SQ SEQUENCE 383 AA; 43882 MW; 9BF8C748B8A8F589 CRC64;
MVVTFLQDLE VLQDALLNNL QKLSAISRRK ESGESKHDNK DSFAAIANEH NDEEEEIEFE
DLVNIIESKV SDFESVLKCS IVEMTYKYPE LKLQWEKSPR YDQCDKLHIV KLDKQMNEDI
YAQLVEELDF VLQFVDWFYC YRLKVKEILR QHHKRDLAWN DEKRDRAIKF HAVDYDKLHQ
GTSSSSSLTS TSMEKASTRE KLLSKTKQLT NNLVRGNQIL QSGILQSDLN LDELRAQTNS
LTQIDDKYTQ FETVFKKTAD LVKVLENASH QEKRDVYLSL GFLLCCVSWV LWRRIFKLPV
KLGLWLLFKF FKGILVTLGL VKSYAGSSSS LQAPSLVLNA PILATTTTSS ATSVEPFASV
SAVSSIQRAV DEAVDRIVSH DEL
