SEC65_SCHPO
ID SEC65_SCHPO Reviewed; 213 AA.
AC O94407;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2012, sequence version 2.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Signal recognition particle sec65 subunit;
GN Name=sec65; ORFNames=SPCC126.15c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=20537132; DOI=10.1186/gb-2010-11-6-r60;
RA Han T.X., Xu X.Y., Zhang M.J., Peng X., Du L.L.;
RT "Global fitness profiling of fission yeast deletion strains by barcode
RT sequencing.";
RL Genome Biol. 11:R60.1-R60.13(2010).
CC -!- FUNCTION: Signal-recognition-particle assembly has a crucial role in
CC targeting secretory proteins to the rough endoplasmic reticulum
CC membrane. It must be involved intimately in the translocation of a wide
CC variety of protein substrates (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Fungal signal recognition particle consists of a 7S RNA
CC molecule (scR1) and at least six protein subunits: srp72, srp68, srp54,
CC sec65, srp21 and srp14. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}.
CC -!- DISRUPTION PHENOTYPE: Leads to sensitivity to camptothecin,
CC thiabendazole, microtubule depolymerizing drugs and DNA damaging
CC agents. {ECO:0000269|PubMed:20537132}.
CC -!- SIMILARITY: Belongs to the SRP19 family. {ECO:0000305}.
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DR EMBL; CU329672; CAA22484.2; -; Genomic_DNA.
DR PIR; T40919; T40919.
DR RefSeq; NP_588458.2; NM_001023449.2.
DR AlphaFoldDB; O94407; -.
DR SMR; O94407; -.
DR BioGRID; 275633; 3.
DR STRING; 4896.SPCC126.15c.1; -.
DR iPTMnet; O94407; -.
DR MaxQB; O94407; -.
DR PaxDb; O94407; -.
DR PRIDE; O94407; -.
DR EnsemblFungi; SPCC126.15c.1; SPCC126.15c.1:pep; SPCC126.15c.
DR GeneID; 2539061; -.
DR KEGG; spo:SPCC126.15c; -.
DR PomBase; SPCC126.15c; sec65.
DR VEuPathDB; FungiDB:SPCC126.15c; -.
DR eggNOG; KOG3198; Eukaryota.
DR HOGENOM; CLU_1397080_0_0_1; -.
DR InParanoid; O94407; -.
DR OMA; NPFKEAM; -.
DR Reactome; R-SPO-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR PRO; PR:O94407; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; ISO:PomBase.
DR GO; GO:0008312; F:7S RNA binding; IBA:GO_Central.
DR GO; GO:0006617; P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; ISO:PomBase.
DR Gene3D; 3.30.56.30; -; 1.
DR InterPro; IPR002778; Signal_recog_particle_SRP19.
DR InterPro; IPR036521; SRP19-like_sf.
DR PANTHER; PTHR17453; PTHR17453; 1.
DR Pfam; PF01922; SRP19; 1.
DR SUPFAM; SSF69695; SSF69695; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Nucleus; Reference proteome; Ribonucleoprotein; RNA-binding;
KW Signal recognition particle.
FT CHAIN 1..213
FT /note="Signal recognition particle sec65 subunit"
FT /id="PRO_0000135210"
FT REGION 176..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 213 AA; 23501 MW; BB6A03076289B33A CRC64;
MISHLLPTTS LTIHMPSIIL YPIYFDKSRP RRFRCVPKDK AILNPLAKNI ADVVRDLGYK
CKLEPLKTHP ADWVNPGRVE MVLPEKIQKK YVINEIAKVL LLRPTVKTDP LSLPIQNVPA
RLPENPPAYP KGVLGNTILP LHSPALSGGG ISENMFQEMM QEMQKQPGLA GGMNPMAALA
GMGGPAPPMP TPQASSSQRK QKSIEPEYDL DLE
