SEC65_YEAST
ID SEC65_YEAST Reviewed; 273 AA.
AC P29478; D6W0H9;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Signal recognition particle subunit SEC65;
GN Name=SEC65; OrderedLocusNames=YML105C; ORFNames=YM8339.14C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1313948; DOI=10.1038/356534a0;
RA Stirling C.J., Hewitt E.W.;
RT "The S. cerevisiae SEC65 gene encodes a component of yeast signal
RT recognition particle with homology to human SRP19.";
RL Nature 356:534-537(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=1313947; DOI=10.1038/356532a0;
RA Hann B.C., Stirling C.J., Walter P.;
RT "SEC65 gene product is a subunit of the yeast signal recognition particle
RT required for its integrity.";
RL Nature 356:532-533(1992).
RN [6]
RP IDENTIFICATION IN THE SRP COMPLEX.
RX PubMed=7925282; DOI=10.1002/j.1460-2075.1994.tb06759.x;
RA Brown J.D., Hann B.C., Medzihradszky K.F., Niwa M., Burlingame A.L.,
RA Walter P.;
RT "Subunits of the Saccharomyces cerevisiae signal recognition particle
RT required for its functional expression.";
RL EMBO J. 13:4390-4400(1994).
CC -!- FUNCTION: Signal-recognition-particle (SRP) assembly has a crucial role
CC in targeting secretory proteins to the rough endoplasmic reticulum (ER)
CC membrane. SRP is required for the cotranslational protein translocation
CC for ER import and preferentially recognizes strongly hydrophobic signal
CC sequences. It is involved in targeting the nascent chain-ribosome (RNC)
CC complex to the ER and is proposed to participate in the arrest of
CC nascent chain elongation during membrane targeting. SEC65 is required
CC for SRP integrity.
CC -!- SUBUNIT: Fungal signal recognition particle (SRP) complex consists of a
CC 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68,
CC SRP54, SEC65, SRP21 and SRP14. {ECO:0000269|PubMed:7925282}.
CC -!- INTERACTION:
CC P29478; P38985: SRP14; NbExp=7; IntAct=EBI-16641, EBI-17977;
CC P29478; P32342: SRP21; NbExp=5; IntAct=EBI-16641, EBI-17984;
CC P29478; P38688: SRP72; NbExp=8; IntAct=EBI-16641, EBI-18011;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR EMBL; X65783; CAA46666.1; -; Genomic_DNA.
DR EMBL; Z49210; CAA89113.1; -; Genomic_DNA.
DR EMBL; AY693145; AAT93164.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09793.1; -; Genomic_DNA.
DR PIR; S21731; S21731.
DR RefSeq; NP_013602.1; NM_001182467.1.
DR AlphaFoldDB; P29478; -.
DR SMR; P29478; -.
DR BioGRID; 35038; 385.
DR ComplexPortal; CPX-609; Signal recognition particle.
DR DIP; DIP-3850N; -.
DR IntAct; P29478; 6.
DR MINT; P29478; -.
DR STRING; 4932.YML105C; -.
DR iPTMnet; P29478; -.
DR MaxQB; P29478; -.
DR PaxDb; P29478; -.
DR PRIDE; P29478; -.
DR EnsemblFungi; YML105C_mRNA; YML105C; YML105C.
DR GeneID; 854866; -.
DR KEGG; sce:YML105C; -.
DR SGD; S000004573; SEC65.
DR VEuPathDB; FungiDB:YML105C; -.
DR eggNOG; KOG3198; Eukaryota.
DR GeneTree; ENSGT00390000004950; -.
DR HOGENOM; CLU_065433_1_1_1; -.
DR InParanoid; P29478; -.
DR OMA; KVKGFKM; -.
DR BioCyc; YEAST:G3O-32689-MON; -.
DR Reactome; R-SCE-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR PRO; PR:P29478; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P29478; protein.
DR GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IDA:SGD.
DR GO; GO:0008312; F:7S RNA binding; IDA:SGD.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IDA:SGD.
DR GO; GO:0006617; P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; IMP:SGD.
DR Gene3D; 3.30.56.30; -; 1.
DR HAMAP; MF_00305; SRP19; 1.
DR InterPro; IPR002778; Signal_recog_particle_SRP19.
DR InterPro; IPR036521; SRP19-like_sf.
DR InterPro; IPR022938; SRP19_arc-type.
DR PANTHER; PTHR17453; PTHR17453; 1.
DR Pfam; PF01922; SRP19; 1.
DR SUPFAM; SSF69695; SSF69695; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; Ribonucleoprotein;
KW Signal recognition particle.
FT CHAIN 1..273
FT /note="Signal recognition particle subunit SEC65"
FT /id="PRO_0000135212"
FT REGION 25..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 273 AA; 31170 MW; 4F4A6CBB9225E385 CRC64;
MPRLEEIDDF NDIDDLDMEL AELDPSLRTP IAPKITPKVV RSQDQENPAF LPGTNNNSNS
NNNSSNEKEQ LSFINPKTGK VERSEAISKK DLEEVKRFQV LYPCYFDINR SHKEGRRVPK
ELAVENPLAK TMADAVRELG ILCIFEGEKC HPQDFGNPGR IRVLFKENGQ LIGAATKFKG
GKRQLMKAVG EYMKRHPTTI ESLREIPYGP DFDNIEFKKI PRVKGFKMNE IVPLHSPFLM
GHPMTKSVYE TPKITAAEKS FKPPKNKYKV VRR
